Molecule image
Summary
| Full name | 8-methyladenosine |
| Short name | m8A |
| New Nomenclature | 8A |
| RNAMods abbreviation | â |
| Enzymes |
Cfr (Staphylococcus sciuri) |
| Found in phylogeny | Eubacteria |
| Found in RNA | rRNA |
| SMILES | CC1=NC2=C(N)N=CN=C2[N]1C3OC(CO)C(O)C3O |
| SMILES (no stereochemical information) | CC1=NC2=C(N)N=CN=C2[N]1C3OC(CO)C(O)C3O |
LC-MS Information
| Sum formula | C11O4N5H15 |
| Monoisotopic mass | 281.1124 |
| Average mass | 281.2719 |
| [M+H]+ | 282.1202 |
| Product ions | 150 |
| Normalized LC elution time * | not available |
| LC elution order/characteristics | not available |
* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.
3D Molecule representation
Download [.mol file for m8A]
Comments
The methyltransferase Cfr making m8A, has only been found in Staphylococcus sciuri so far, but it has been investigated in E. coli by expression of Cfr from a plasmid coded gene. It methylates A2503 in E. coli 23S RNA to m8A.
Also see:
Identification of 8-methyladenosine as the modification catalyzed by the
radical SAM methyltransferase Cfr that confers antibiotic resistance in
bacteria. Giessing AM, Jensen SS, Rasmussen A, Hansen LH, Gondela A, Long K, Vester B,
Kirpekar F. RNA. 2009 Feb;15(2):327-36.
PMID: 19144912
Chemical groups contained
| Type | Subtype |
|---|---|
| methyl group | methyl at aromatic C |
Reactions producing 8-methyladenosine
| Name |
|---|
| A:m8A |
Reactions starting from 8-methyladenosine
| Name |
|---|
| m8A:m2,8A |
Last modification of this entry: Oct. 6, 2017