Molecule image



Full nameN6,N6-dimethyladenosine
Short namem6,6A
New Nomenclature66A
RNAMods abbreviationζ
Enzymes RsmA (Escherichia coli)
RsmA (Saccharomyces cerevisiae)
RsmA (Thermus thermophilus)
Found in phylogenyEubacteria, Eukaryota
Found in RNArRNA
PubChem ID72266
SMILES (no stereochemical information)C3(OC(CO)C(O)C(O)3)N2C=NC1=C2N=CN=C1N(C)(C)

LC-MS Information

Sum formulaC12O4N5H17
Monoisotopic mass295.1281
Average mass295.2989
Product ions164
Normalized LC elution time *1,86 (Kellner 2014); 2,57 (Kellner 2014)
LC elution order/characteristicsafter m6A (Kellner 2014, Kellner 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

3D Molecule representation

Download [.mol file for m6,6A]

Chemical groups contained

methyl groupmethyl at other N
methyl groupsecond methyl at other N

Reactions producing N6,N6-dimethyladenosine


Reactions starting from N6,N6-dimethyladenosine


Occurrence in PDB structures

PDB-ID chain residue
1wts MA6/8
1wts MA6/9
1wtt MA6/8
1wtt MA6/9
2i1c y MA6/1518
2i1c y MA6/1519

Last modification of this entry: Oct. 6, 2017

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