CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of HotKnots - scored higher in this pairwise comparison

  4. Performance of Pknots - scored lower in this pairwise comparison

  5. Compile and download dataset for HotKnots & Pknots [.zip] - may take several seconds...


Overview

Metric HotKnots Pknots
MCC 0.804 > 0.740
Average MCC ± 95% Confidence Intervals 0.818 ± 0.051 > 0.768 ± 0.057
Sensitivity 0.815 > 0.763
Positive Predictive Value 0.801 > 0.726
Total TP 1540 > 1443
Total TN 102136 > 102072
Total FP 630 < 747
Total FP CONTRA 107 < 179
Total FP INCONS 276 < 365
Total FP COMP 247 > 203
Total FN 350 < 447
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of HotKnots and Pknots. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for HotKnots and Pknots).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for HotKnots and Pknots).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for HotKnots and Pknots. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for HotKnots and Pknots).

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Performance of HotKnots - scored higher in this pairwise comparison

1. Total counts & total scores for HotKnots

Total Base Pair Counts
Total TP 1540
Total TN 102136
Total FP 630
Total FP CONTRA 107
Total FP INCONS 276
Total FP COMP 247
Total FN 350
Total Scores
MCC 0.804
Average MCC ± 95% Confidence Intervals 0.818 ± 0.051
Sensitivity 0.815
Positive Predictive Value 0.801
Nr of predictions 116

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2. Individual counts for HotKnots [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 175 0 0 0 0 0
2K63_A - 1.00 1.00 1.00 9 159 0 0 0 0 0
2K66_A - 1.00 1.00 1.00 9 80 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KDQ_B 1.00 1.00 1.00 10 164 0 0 0 0 0
2KE6_A 1.00 1.00 1.00 18 449 1 0 0 1 0
2KEZ_A - 1.00 1.00 1.00 8 94 0 0 0 0 0
2KFC_A - -0.03 0.00 0.00 0 229 7 1 6 0 8
2KGP_A - 1.00 1.00 1.00 9 112 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 157 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.91 0.87 0.95 20 2003 9 1 0 8 3
2KU0_A - 0.96 0.92 1.00 11 281 0 0 0 0 1
2KUR_A 1.00 1.00 1.00 19 448 0 0 0 0 0
2KUU_A 1.00 1.00 1.00 18 429 1 0 0 1 0
2KUV_A 1.00 1.00 1.00 19 420 0 0 0 0 0
2KUW_A 1.00 1.00 1.00 18 452 1 0 0 1 0
2KX8_A 1.00 1.00 1.00 16 355 0 0 0 0 0
2KXM_A - 1.00 1.00 1.00 8 141 0 0 0 0 0
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_A 1.00 1.00 1.00 23 740 0 0 0 0 0
2L1F_B 1.00 1.00 1.00 24 767 0 0 0 0 0
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 1.00 1.00 1.00 14 225 0 0 0 0 0
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A 0.64 0.61 0.69 11 512 5 0 5 0 7
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 1.00 1.00 1.00 29 2411 8 0 0 8 0
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.95 0.91 1.00 10 197 1 0 0 1 1
2RP0_A - 0.84 0.71 1.00 5 111 0 0 0 0 2
2RPT_A - 1.00 1.00 1.00 7 65 0 0 0 0 0
2RRC_A - 1.00 1.00 1.00 7 68 0 0 0 0 0
2WRQ_Y 1.00 1.00 1.00 9 1143 14 0 0 14 0
2WW9_F - 0.68 0.75 0.67 6 103 4 1 2 1 2
2WW9_D - 0.08 0.10 0.08 1 729 15 2 9 4 9
2WW9_E - 0.89 0.80 1.00 4 168 0 0 0 0 1
2WWQ_V 1.00 1.00 1.00 19 1185 5 0 0 5 0
2XKV_B 0.64 0.73 0.57 8 1821 25 0 6 19 3
2XQD_Y 0.98 0.95 1.00 20 1109 1 0 0 1 1
2XXA_G 0.35 0.34 0.38 12 2013 21 1 19 1 23
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
2YIE_X - 0.53 0.57 0.50 4 536 6 1 3 2 3
2ZY6_A - 0.66 0.78 0.58 7 264 5 3 2 0 2
2ZZM_B 0.25 0.27 0.25 4 1342 20 3 9 8 11
2ZZN_D 0.93 0.95 0.91 21 961 3 2 0 1 1
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3A3A_A 0.97 0.93 1.00 28 1472 0 0 0 0 2
3ADB_C - 0.86 0.85 0.88 28 1787 5 0 4 1 5
3AKZ_H 0.48 0.50 0.48 10 1106 13 4 7 2 10
3AM1_B - 0.86 0.86 0.86 25 1437 4 0 4 0 4
3AMU_B 0.81 0.79 0.83 15 1139 6 0 3 3 4
3DW4_A - -0.03 0.00 0.00 0 136 6 1 3 2 6
3G4S_9 0.78 0.77 0.80 20 2711 14 2 3 9 6
3GCA_A - 0.84 0.71 1.00 5 153 0 0 0 0 2
3GX2_A 0.81 0.79 0.85 22 1423 5 2 2 1 6
3HAY_E - 1.00 1.00 1.00 14 910 7 0 0 7 0
3HJW_D - 1.00 1.00 1.00 16 593 2 0 0 2 0
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3ID5_D - 0.59 0.60 0.60 3 224 5 0 2 3 2
3IVN_B 0.91 0.83 1.00 19 884 0 0 0 0 4
3IWN_A 0.69 0.68 0.70 19 1445 9 1 7 1 9
3IZF_C 0.89 0.91 0.86 32 2603 9 0 5 4 3
3J0L_a - 0.55 0.64 0.50 7 397 8 5 2 1 4
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_h - 0.87 0.81 0.93 26 2112 4 1 1 2 6
3J0L_7 - 0.38 0.50 0.31 5 503 12 4 7 1 5
3J0L_g - -0.01 0.00 0.00 0 173 5 1 2 2 2
3J0L_2 - 0.34 0.35 0.35 9 2224 23 2 15 6 17
3J16_L 0.31 0.33 0.30 7 1136 16 3 13 0 14
3JYV_7 -0.02 0.00 0.00 0 1089 22 4 18 0 20
3JYX_4 0.61 0.83 0.45 10 4734 31 10 2 19 2
3JYX_3 0.77 0.80 0.75 12 2362 27 0 4 23 3
3KTW_C - 0.96 0.96 0.96 24 1755 9 0 1 8 1
3LA5_A 0.91 0.84 1.00 21 933 0 0 0 0 4
3NDB_M - 1.00 1.00 1.00 44 3670 4 0 0 4 0
3NKB_B - 0.69 0.74 0.67 14 714 7 0 7 0 5
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.85 0.78 0.94 29 2247 6 0 2 4 8
3O58_2 0.87 0.87 0.87 27 2723 12 0 4 8 4
3O58_3 0.26 0.36 0.19 8 4722 35 16 18 1 14
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.81 0.80 0.82 40 4791 11 3 6 2 10
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.77 0.76 0.79 22 1505 6 4 2 0 7
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.49 0.55 0.46 12 1250 14 3 11 0 10
3UZL_B 0.49 0.50 0.50 8 1277 15 0 8 7 8
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
4A1C_2 0.19 0.25 0.15 5 4483 42 12 16 14 15
4A1C_3 0.88 0.86 0.89 32 2727 7 0 4 3 5
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.60 0.59 0.63 17 1410 11 4 6 1 12
4ENB_A 1.00 1.00 1.00 15 457 0 0 0 0 0
4ENC_A 0.97 1.00 0.94 15 480 1 1 0 0 0

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Performance of Pknots - scored lower in this pairwise comparison

1. Total counts & total scores for Pknots

Total Base Pair Counts
Total TP 1443
Total TN 102072
Total FP 747
Total FP CONTRA 179
Total FP INCONS 365
Total FP COMP 203
Total FN 447
Total Scores
MCC 0.740
Average MCC ± 95% Confidence Intervals 0.768 ± 0.057
Sensitivity 0.763
Positive Predictive Value 0.726
Nr of predictions 116

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2. Individual counts for Pknots [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 0.90 0.82 1.00 9 177 0 0 0 0 2
2K63_A - 1.00 1.00 1.00 9 159 0 0 0 0 0
2K66_A - 1.00 1.00 1.00 9 80 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KDQ_B 1.00 1.00 1.00 10 164 0 0 0 0 0
2KE6_A 1.00 1.00 1.00 18 449 1 0 0 1 0
2KEZ_A - 1.00 1.00 1.00 8 94 0 0 0 0 0
2KFC_A - 0.17 0.25 0.17 2 224 10 3 7 0 6
2KGP_A - 0.81 0.67 1.00 6 115 1 0 0 1 3
2KMJ_A - 1.00 1.00 1.00 11 157 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.79 0.83 0.76 19 1999 13 5 1 7 4
2KU0_A - -0.04 0.00 0.00 0 282 10 0 10 0 12
2KUR_A 1.00 1.00 1.00 19 448 0 0 0 0 0
2KUU_A 1.00 1.00 1.00 18 429 1 0 0 1 0
2KUV_A 1.00 1.00 1.00 19 420 0 0 0 0 0
2KUW_A 1.00 1.00 1.00 18 452 1 0 0 1 0
2KX8_A 1.00 1.00 1.00 16 355 0 0 0 0 0
2KXM_A - -0.04 0.00 0.00 0 144 5 0 5 0 8
2KZL_A - 0.66 0.69 0.64 9 506 6 2 3 1 4
2L1F_A 1.00 1.00 1.00 23 740 0 0 0 0 0
2L1F_B 1.00 1.00 1.00 24 767 0 0 0 0 0
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 0.96 0.93 1.00 13 226 0 0 0 0 1
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 0.94 0.94 0.94 17 339 2 0 1 1 1
2LA5_A - 0.77 0.71 0.83 5 269 1 0 1 0 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A 0.83 0.83 0.83 15 510 3 2 1 0 3
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 0.48 0.55 0.43 16 2403 23 9 12 2 13
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.38 0.27 0.60 3 202 3 0 2 1 8
2RP0_A - 0.87 1.00 0.78 7 107 2 2 0 0 0
2RPT_A - 1.00 1.00 1.00 7 65 0 0 0 0 0
2RRC_A - 0.92 0.86 1.00 6 69 0 0 0 0 1
2WRQ_Y 1.00 1.00 1.00 9 1143 13 0 0 13 0
2WW9_F - 0.68 0.75 0.67 6 103 4 1 2 1 2
2WW9_D - 0.52 0.60 0.46 6 728 13 5 2 6 4
2WW9_E - 0.79 0.80 0.80 4 167 3 0 1 2 1
2WWQ_V 0.29 0.32 0.29 6 1183 16 3 12 1 13
2XKV_B 0.21 0.27 0.17 3 1817 33 3 12 18 8
2XQD_Y 0.98 0.95 1.00 20 1109 1 0 0 1 1
2XXA_G 0.32 0.34 0.32 12 2008 26 1 24 1 23
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.70 0.67 0.75 12 890 6 0 4 2 6
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
2YIE_X - 0.53 0.57 0.50 4 536 6 1 3 2 3
2ZY6_A - 0.66 0.78 0.58 7 264 5 3 2 0 2
2ZZM_B 0.18 0.20 0.19 3 1342 23 4 9 10 12
2ZZN_D 0.95 0.95 0.95 21 962 1 1 0 0 1
3A2K_C 0.50 0.55 0.48 12 1083 13 3 10 0 10
3A3A_A 0.97 0.93 1.00 28 1472 0 0 0 0 2
3ADB_C - 0.98 0.97 1.00 32 1787 0 0 0 0 1
3AKZ_H 1.00 1.00 1.00 20 1107 2 0 0 2 0
3AM1_B - 1.00 1.00 1.00 29 1437 0 0 0 0 0
3AMU_B 1.00 1.00 1.00 19 1138 3 0 0 3 0
3DW4_A - 0.91 0.83 1.00 5 135 0 0 0 0 1
3G4S_9 0.37 0.46 0.31 12 2697 27 14 13 0 14
3GCA_A - 0.66 0.57 0.80 4 153 3 0 1 2 3
3GX2_A 0.55 0.57 0.55 16 1420 14 4 9 1 12
3HAY_E - 1.00 1.00 1.00 14 910 6 0 0 6 0
3HJW_D - 1.00 1.00 1.00 16 593 3 0 0 3 0
3IAB_R - 1.00 1.00 1.00 12 373 4 0 0 4 0
3ID5_D - -0.02 0.00 0.00 0 224 5 1 4 0 5
3IVN_B 0.91 0.87 0.95 20 882 1 0 1 0 3
3IWN_A 0.22 0.21 0.25 6 1448 18 2 16 0 22
3IZF_C 0.89 0.91 0.86 32 2603 9 0 5 4 3
3J0L_a - 0.35 0.36 0.36 4 400 8 3 4 1 7
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_1 - 0.73 0.62 0.89 8 475 3 0 1 2 5
3J0L_h - 0.67 0.66 0.70 21 2110 11 4 5 2 11
3J0L_7 - 0.38 0.50 0.31 5 503 12 3 8 1 5
3J0L_g - -0.02 0.00 0.00 0 171 5 3 2 0 2
3J0L_2 - 0.44 0.46 0.43 12 2222 23 1 15 7 14
3J16_L 0.41 0.43 0.41 9 1137 13 1 12 0 12
3JYV_7 0.97 0.95 1.00 19 1092 2 0 0 2 1
3JYX_4 0.33 0.58 0.19 7 4720 41 22 7 12 5
3JYX_3 0.37 0.53 0.27 8 2348 26 17 5 4 7
3KTW_C - 0.63 0.68 0.59 17 1751 18 3 9 6 8
3LA5_A 0.94 0.88 1.00 22 932 0 0 0 0 3
3NDB_M - 0.92 0.93 0.91 41 3669 8 3 1 4 3
3NKB_B - 0.64 0.68 0.62 13 714 8 0 8 0 6
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.84 0.81 0.88 30 2244 9 0 4 5 7
3O58_2 0.93 0.90 0.97 28 2725 3 0 1 2 3
3O58_3 0.32 0.45 0.24 10 4722 39 18 14 7 12
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.64 0.64 0.65 32 4791 19 4 13 2 18
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 0.87 0.88 0.88 7 221 5 0 1 4 1
3RKF_A 0.91 0.88 0.95 21 844 1 0 1 0 3
3SD1_A 0.78 0.76 0.81 22 1506 5 1 4 0 7
3SIU_F - 0.86 0.75 1.00 6 139 0 0 0 0 2
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.33 0.36 0.32 8 1251 17 4 13 0 14
3UZL_B 1.00 1.00 1.00 16 1277 7 0 0 7 0
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
4A1C_2 0.33 0.40 0.29 8 4488 36 9 11 16 12
4A1C_3 0.28 0.30 0.28 11 2723 30 4 25 1 26
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.19 0.21 0.21 6 1409 23 3 19 1 23
4ENB_A 1.00 1.00 1.00 15 457 2 0 0 2 0
4ENC_A 0.97 1.00 0.94 15 480 1 1 0 0 0

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.