CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of HotKnots - scored higher in this pairwise comparison

  4. Performance of RNAshapes - scored lower in this pairwise comparison

  5. Compile and download dataset for HotKnots & RNAshapes [.zip] - may take several seconds...


Overview

Metric HotKnots RNAshapes
MCC 0.772 > 0.759
Average MCC ± 95% Confidence Intervals 0.811 ± 0.052 > 0.791 ± 0.053
Sensitivity 0.793 > 0.770
Positive Predictive Value 0.757 > 0.755
Total TP 1614 > 1567
Total TN 149903 < 149960
Total FP 783 > 782
Total FP CONTRA 180 > 148
Total FP INCONS 338 < 360
Total FP COMP 265 < 274
Total FN 422 < 469
P-value 4.48494289621e-08

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Performance plots


  1. Comparison of performance of HotKnots and RNAshapes. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for HotKnots and RNAshapes).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for HotKnots and RNAshapes).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for HotKnots and RNAshapes. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for HotKnots and RNAshapes).

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Performance of HotKnots - scored higher in this pairwise comparison

1. Total counts & total scores for HotKnots

Total Base Pair Counts
Total TP 1614
Total TN 149903
Total FP 783
Total FP CONTRA 180
Total FP INCONS 338
Total FP COMP 265
Total FN 422
Total Scores
MCC 0.772
Average MCC ± 95% Confidence Intervals 0.811 ± 0.052
Sensitivity 0.793
Positive Predictive Value 0.757
Nr of predictions 118

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2. Individual counts for HotKnots [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 175 0 0 0 0 0
2K63_A - 1.00 1.00 1.00 9 159 0 0 0 0 0
2K66_A - 1.00 1.00 1.00 9 80 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KDQ_B 1.00 1.00 1.00 10 164 0 0 0 0 0
2KE6_A 1.00 1.00 1.00 18 449 1 0 0 1 0
2KEZ_A - 1.00 1.00 1.00 8 94 0 0 0 0 0
2KFC_A - -0.03 0.00 0.00 0 229 7 1 6 0 8
2KGP_A - 1.00 1.00 1.00 9 112 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 157 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.91 0.87 0.95 20 2003 9 1 0 8 3
2KU0_A - 0.96 0.92 1.00 11 281 0 0 0 0 1
2KUR_A 1.00 1.00 1.00 19 448 0 0 0 0 0
2KUU_A 1.00 1.00 1.00 18 429 1 0 0 1 0
2KUV_A 1.00 1.00 1.00 19 420 0 0 0 0 0
2KUW_A 1.00 1.00 1.00 18 452 1 0 0 1 0
2KX8_A 1.00 1.00 1.00 16 355 0 0 0 0 0
2KXM_A - 1.00 1.00 1.00 8 141 0 0 0 0 0
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_A 1.00 1.00 1.00 23 740 0 0 0 0 0
2L1F_B 1.00 1.00 1.00 24 767 0 0 0 0 0
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 1.00 1.00 1.00 14 225 0 0 0 0 0
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A 0.64 0.61 0.69 11 512 5 0 5 0 7
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 1.00 1.00 1.00 29 2411 8 0 0 8 0
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.95 0.91 1.00 10 197 1 0 0 1 1
2RP0_A - 0.84 0.71 1.00 5 111 0 0 0 0 2
2RPT_A - 1.00 1.00 1.00 7 65 0 0 0 0 0
2RRC_A - 1.00 1.00 1.00 7 68 0 0 0 0 0
2WRQ_Y 1.00 1.00 1.00 9 1143 14 0 0 14 0
2WW9_D - 0.08 0.10 0.08 1 729 15 2 9 4 9
2WW9_E - 0.89 0.80 1.00 4 168 0 0 0 0 1
2WW9_F - 0.68 0.75 0.67 6 103 4 1 2 1 2
2WWQ_V 1.00 1.00 1.00 19 1185 5 0 0 5 0
2XKV_B 0.64 0.73 0.57 8 1821 25 0 6 19 3
2XQD_Y 0.98 0.95 1.00 20 1109 1 0 0 1 1
2XXA_G 0.35 0.34 0.38 12 2013 21 1 19 1 23
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_X - 0.53 0.57 0.50 4 536 6 1 3 2 3
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
2ZY6_A - 0.66 0.78 0.58 7 264 5 3 2 0 2
2ZZM_B 0.25 0.27 0.25 4 1342 20 3 9 8 11
2ZZN_D 0.93 0.95 0.91 21 961 3 2 0 1 1
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3A3A_A 0.97 0.93 1.00 28 1472 0 0 0 0 2
3ADB_C - 0.86 0.85 0.88 28 1787 5 0 4 1 5
3AKZ_H 0.48 0.50 0.48 10 1106 13 4 7 2 10
3AM1_B - 0.86 0.86 0.86 25 1437 4 0 4 0 4
3AMU_B 0.81 0.79 0.83 15 1139 6 0 3 3 4
3DW4_A - -0.03 0.00 0.00 0 136 6 1 3 2 6
3G4S_9 0.78 0.77 0.80 20 2711 14 2 3 9 6
3GCA_A - 0.84 0.71 1.00 5 153 0 0 0 0 2
3GX2_A 0.81 0.79 0.85 22 1423 5 2 2 1 6
3HAY_E - 1.00 1.00 1.00 14 910 7 0 0 7 0
3HJW_D - 1.00 1.00 1.00 16 593 2 0 0 2 0
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3ID5_D - 0.59 0.60 0.60 3 224 5 0 2 3 2
3IVN_B 0.91 0.83 1.00 19 884 0 0 0 0 4
3IWN_A 0.69 0.68 0.70 19 1445 9 1 7 1 9
3IYQ_A 0.24 0.33 0.17 17 22341 96 47 35 14 34
3IZ4_A 0.56 0.60 0.52 57 25426 57 26 27 4 38
3IZF_C 0.89 0.91 0.86 32 2603 9 0 5 4 3
3J0L_h - 0.87 0.81 0.93 26 2112 4 1 1 2 6
3J0L_g - -0.01 0.00 0.00 0 173 5 1 2 2 2
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_a - 0.55 0.64 0.50 7 397 8 5 2 1 4
3J0L_2 - 0.34 0.35 0.35 9 2224 23 2 15 6 17
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_7 - 0.38 0.50 0.31 5 503 12 4 7 1 5
3J16_L 0.31 0.33 0.30 7 1136 16 3 13 0 14
3JYV_7 -0.02 0.00 0.00 0 1089 22 4 18 0 20
3JYX_3 0.77 0.80 0.75 12 2362 27 0 4 23 3
3JYX_4 0.61 0.83 0.45 10 4734 31 10 2 19 2
3KTW_C - 0.96 0.96 0.96 24 1755 9 0 1 8 1
3LA5_A 0.91 0.84 1.00 21 933 0 0 0 0 4
3NDB_M - 1.00 1.00 1.00 44 3670 4 0 0 4 0
3NKB_B - 0.69 0.74 0.67 14 714 7 0 7 0 5
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.85 0.78 0.94 29 2247 6 0 2 4 8
3O58_2 0.87 0.87 0.87 27 2723 12 0 4 8 4
3O58_3 0.26 0.36 0.19 8 4722 35 16 18 1 14
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.81 0.80 0.82 40 4791 11 3 6 2 10
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.77 0.76 0.79 22 1505 6 4 2 0 7
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.49 0.55 0.46 12 1250 14 3 11 0 10
3UZL_B 0.49 0.50 0.50 8 1277 15 0 8 7 8
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
4A1C_2 0.19 0.25 0.15 5 4483 42 12 16 14 15
4A1C_3 0.88 0.86 0.89 32 2727 7 0 4 3 5
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.60 0.59 0.63 17 1410 11 4 6 1 12
4ENB_A 1.00 1.00 1.00 15 457 0 0 0 0 0
4ENC_A 0.97 1.00 0.94 15 480 1 1 0 0 0

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Performance of RNAshapes - scored lower in this pairwise comparison

1. Total counts & total scores for RNAshapes

Total Base Pair Counts
Total TP 1567
Total TN 149960
Total FP 782
Total FP CONTRA 148
Total FP INCONS 360
Total FP COMP 274
Total FN 469
Total Scores
MCC 0.759
Average MCC ± 95% Confidence Intervals 0.791 ± 0.053
Sensitivity 0.770
Positive Predictive Value 0.755
Nr of predictions 118

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2. Individual counts for RNAshapes [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 175 0 0 0 0 0
2K63_A - 1.00 1.00 1.00 9 159 0 0 0 0 0
2K66_A - 1.00 1.00 1.00 9 80 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KDQ_B 1.00 1.00 1.00 10 164 0 0 0 0 0
2KE6_A 1.00 1.00 1.00 18 449 1 0 0 1 0
2KEZ_A - 1.00 1.00 1.00 8 94 0 0 0 0 0
2KFC_A - -0.03 0.00 0.00 0 229 7 1 6 0 8
2KGP_A - 1.00 1.00 1.00 9 112 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 157 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.91 0.87 0.95 20 2003 9 1 0 8 3
2KU0_A - 0.96 0.92 1.00 11 281 0 0 0 0 1
2KUR_A 1.00 1.00 1.00 19 448 0 0 0 0 0
2KUU_A 1.00 1.00 1.00 18 429 1 0 0 1 0
2KUV_A 1.00 1.00 1.00 19 420 0 0 0 0 0
2KUW_A 1.00 1.00 1.00 18 452 1 0 0 1 0
2KX8_A 0.97 0.94 1.00 15 356 0 0 0 0 1
2KXM_A - 0.93 0.88 1.00 7 142 0 0 0 0 1
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_A 0.89 0.87 0.91 20 741 2 0 2 0 3
2L1F_B 0.98 0.96 1.00 23 768 0 0 0 0 1
2L2K_A - 0.97 0.94 1.00 16 333 0 0 0 0 1
2L3C_B - 1.00 1.00 1.00 14 225 0 0 0 0 0
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 0.98 0.97 1.00 29 956 0 0 0 0 1
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A 0.64 0.61 0.69 11 512 5 0 5 0 7
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 1.00 1.00 1.00 29 2411 6 0 0 6 0
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.95 0.91 1.00 10 197 1 0 0 1 1
2RP0_A - 0.84 0.71 1.00 5 111 0 0 0 0 2
2RPT_A - 1.00 1.00 1.00 7 65 0 0 0 0 0
2RRC_A - 0.83 0.71 1.00 5 70 0 0 0 0 2
2WRQ_Y 1.00 1.00 1.00 9 1143 13 0 0 13 0
2WW9_D - 0.11 0.10 0.14 1 734 11 2 4 5 9
2WW9_E - 0.89 0.80 1.00 4 168 0 0 0 0 1
2WW9_F - 0.68 0.75 0.67 6 103 4 1 2 1 2
2WWQ_V 1.00 1.00 1.00 19 1185 5 0 0 5 0
2XKV_B 0.64 0.73 0.57 8 1821 24 0 6 18 3
2XQD_Y 0.70 0.67 0.74 14 1110 6 0 5 1 7
2XXA_G 1.00 1.00 1.00 35 2010 1 0 0 1 0
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_X - 0.33 0.43 0.27 3 533 11 3 5 3 4
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
2ZY6_A - 0.69 0.78 0.64 7 265 5 2 2 1 2
2ZZM_B 0.05 0.07 0.06 1 1340 25 4 13 8 14
2ZZN_D 0.77 0.77 0.77 17 962 5 1 4 0 5
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3A3A_A 0.84 0.80 0.89 24 1473 3 0 3 0 6
3ADB_C - 0.71 0.70 0.74 23 1788 8 0 8 0 10
3AKZ_H 0.78 0.80 0.76 16 1106 7 2 3 2 4
3AM1_B - 0.71 0.72 0.70 21 1436 9 3 6 0 8
3AMU_B 0.81 0.79 0.83 15 1139 6 0 3 3 4
3DW4_A - -0.03 0.00 0.00 0 137 3 0 3 0 6
3G4S_9 0.58 0.58 0.60 15 2711 19 2 8 9 11
3GCA_A - 0.84 0.71 1.00 5 153 0 0 0 0 2
3GX2_A 0.91 0.89 0.93 25 1422 3 1 1 1 3
3HAY_E - 1.00 1.00 1.00 14 910 5 0 0 5 0
3HJW_D - 1.00 1.00 1.00 16 593 0 0 0 0 0
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3ID5_D - 0.59 0.60 0.60 3 224 5 0 2 3 2
3IVN_B 0.91 0.83 1.00 19 884 0 0 0 0 4
3IWN_A 0.83 0.79 0.88 22 1447 3 1 2 0 6
3IYQ_A 0.19 0.27 0.14 14 22340 95 43 43 9 37
3IZ4_A 0.57 0.58 0.56 55 25437 50 16 28 6 40
3IZF_C 0.76 0.77 0.75 27 2604 14 0 9 5 8
3J0L_h - 0.87 0.81 0.93 26 2112 4 1 1 2 6
3J0L_g - -0.01 0.00 0.00 0 174 4 0 2 2 2
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_a - 0.18 0.18 0.22 2 402 8 3 4 1 9
3J0L_2 - 0.46 0.46 0.48 12 2225 20 1 12 7 14
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_7 - -0.02 0.00 0.00 0 507 12 2 10 0 10
3J16_L 0.59 0.57 0.63 12 1140 7 0 7 0 9
3JYV_7 -0.02 0.00 0.00 0 1092 20 1 18 1 20
3JYX_3 0.77 0.80 0.75 12 2362 26 0 4 22 3
3JYX_4 0.61 0.83 0.45 10 4734 35 10 2 23 2
3KTW_C - 0.57 0.60 0.56 15 1753 19 2 10 7 10
3LA5_A 0.89 0.80 1.00 20 934 0 0 0 0 5
3NDB_M - 0.98 0.98 0.98 43 3670 5 0 1 4 1
3NKB_B - 0.75 0.74 0.78 14 717 6 0 4 2 5
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.84 0.76 0.93 28 2248 5 1 1 3 9
3O58_2 0.73 0.74 0.72 23 2722 18 0 9 9 8
3O58_3 0.41 0.50 0.34 11 4732 38 6 15 17 11
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.80 0.80 0.80 40 4790 12 3 7 2 10
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.77 0.76 0.79 22 1505 6 4 2 0 7
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.49 0.55 0.46 12 1250 14 3 11 0 10
3UZL_B 0.43 0.50 0.38 8 1272 17 4 9 4 8
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
4A1C_2 0.19 0.25 0.16 5 4484 41 11 16 14 15
4A1C_3 0.86 0.84 0.89 31 2728 7 0 4 3 6
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.60 0.59 0.63 17 1410 11 4 6 1 12
4ENB_A 0.85 0.73 1.00 11 461 2 0 0 2 4
4ENC_A 0.37 0.33 0.45 5 485 7 0 6 1 10

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.