CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of HotKnots - scored higher in this pairwise comparison

  4. Performance of RNAsubopt - scored lower in this pairwise comparison

  5. Compile and download dataset for HotKnots & RNAsubopt [.zip] - may take several seconds...


Overview

Metric HotKnots RNAsubopt
MCC 0.772 > 0.738
Average MCC ± 95% Confidence Intervals 0.811 ± 0.052 > 0.770 ± 0.057
Sensitivity 0.793 > 0.757
Positive Predictive Value 0.757 > 0.726
Total TP 1614 > 1542
Total TN 149903 < 149912
Total FP 783 < 846
Total FP CONTRA 180 < 185
Total FP INCONS 338 < 396
Total FP COMP 265 = 265
Total FN 422 < 494
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of HotKnots and RNAsubopt. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for HotKnots and RNAsubopt).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for HotKnots and RNAsubopt).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for HotKnots and RNAsubopt. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for HotKnots and RNAsubopt).

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Performance of HotKnots - scored higher in this pairwise comparison

1. Total counts & total scores for HotKnots

Total Base Pair Counts
Total TP 1614
Total TN 149903
Total FP 783
Total FP CONTRA 180
Total FP INCONS 338
Total FP COMP 265
Total FN 422
Total Scores
MCC 0.772
Average MCC ± 95% Confidence Intervals 0.811 ± 0.052
Sensitivity 0.793
Positive Predictive Value 0.757
Nr of predictions 118

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2. Individual counts for HotKnots [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 175 0 0 0 0 0
2K63_A - 1.00 1.00 1.00 9 159 0 0 0 0 0
2K66_A - 1.00 1.00 1.00 9 80 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KDQ_B 1.00 1.00 1.00 10 164 0 0 0 0 0
2KE6_A 1.00 1.00 1.00 18 449 1 0 0 1 0
2KEZ_A - 1.00 1.00 1.00 8 94 0 0 0 0 0
2KFC_A - -0.03 0.00 0.00 0 229 7 1 6 0 8
2KGP_A - 1.00 1.00 1.00 9 112 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 157 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.91 0.87 0.95 20 2003 9 1 0 8 3
2KU0_A - 0.96 0.92 1.00 11 281 0 0 0 0 1
2KUR_A 1.00 1.00 1.00 19 448 0 0 0 0 0
2KUU_A 1.00 1.00 1.00 18 429 1 0 0 1 0
2KUV_A 1.00 1.00 1.00 19 420 0 0 0 0 0
2KUW_A 1.00 1.00 1.00 18 452 1 0 0 1 0
2KX8_A 1.00 1.00 1.00 16 355 0 0 0 0 0
2KXM_A - 1.00 1.00 1.00 8 141 0 0 0 0 0
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_A 1.00 1.00 1.00 23 740 0 0 0 0 0
2L1F_B 1.00 1.00 1.00 24 767 0 0 0 0 0
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 1.00 1.00 1.00 14 225 0 0 0 0 0
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A 0.64 0.61 0.69 11 512 5 0 5 0 7
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 1.00 1.00 1.00 29 2411 8 0 0 8 0
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.95 0.91 1.00 10 197 1 0 0 1 1
2RP0_A - 0.84 0.71 1.00 5 111 0 0 0 0 2
2RPT_A - 1.00 1.00 1.00 7 65 0 0 0 0 0
2RRC_A - 1.00 1.00 1.00 7 68 0 0 0 0 0
2WRQ_Y 1.00 1.00 1.00 9 1143 14 0 0 14 0
2WW9_D - 0.08 0.10 0.08 1 729 15 2 9 4 9
2WW9_E - 0.89 0.80 1.00 4 168 0 0 0 0 1
2WW9_F - 0.68 0.75 0.67 6 103 4 1 2 1 2
2WWQ_V 1.00 1.00 1.00 19 1185 5 0 0 5 0
2XKV_B 0.64 0.73 0.57 8 1821 25 0 6 19 3
2XQD_Y 0.98 0.95 1.00 20 1109 1 0 0 1 1
2XXA_G 0.35 0.34 0.38 12 2013 21 1 19 1 23
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_X - 0.53 0.57 0.50 4 536 6 1 3 2 3
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
2ZY6_A - 0.66 0.78 0.58 7 264 5 3 2 0 2
2ZZM_B 0.25 0.27 0.25 4 1342 20 3 9 8 11
2ZZN_D 0.93 0.95 0.91 21 961 3 2 0 1 1
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3A3A_A 0.97 0.93 1.00 28 1472 0 0 0 0 2
3ADB_C - 0.86 0.85 0.88 28 1787 5 0 4 1 5
3AKZ_H 0.48 0.50 0.48 10 1106 13 4 7 2 10
3AM1_B - 0.86 0.86 0.86 25 1437 4 0 4 0 4
3AMU_B 0.81 0.79 0.83 15 1139 6 0 3 3 4
3DW4_A - -0.03 0.00 0.00 0 136 6 1 3 2 6
3G4S_9 0.78 0.77 0.80 20 2711 14 2 3 9 6
3GCA_A - 0.84 0.71 1.00 5 153 0 0 0 0 2
3GX2_A 0.81 0.79 0.85 22 1423 5 2 2 1 6
3HAY_E - 1.00 1.00 1.00 14 910 7 0 0 7 0
3HJW_D - 1.00 1.00 1.00 16 593 2 0 0 2 0
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3ID5_D - 0.59 0.60 0.60 3 224 5 0 2 3 2
3IVN_B 0.91 0.83 1.00 19 884 0 0 0 0 4
3IWN_A 0.69 0.68 0.70 19 1445 9 1 7 1 9
3IYQ_A 0.24 0.33 0.17 17 22341 96 47 35 14 34
3IZ4_A 0.56 0.60 0.52 57 25426 57 26 27 4 38
3IZF_C 0.89 0.91 0.86 32 2603 9 0 5 4 3
3J0L_h - 0.87 0.81 0.93 26 2112 4 1 1 2 6
3J0L_g - -0.01 0.00 0.00 0 173 5 1 2 2 2
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_a - 0.55 0.64 0.50 7 397 8 5 2 1 4
3J0L_2 - 0.34 0.35 0.35 9 2224 23 2 15 6 17
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_7 - 0.38 0.50 0.31 5 503 12 4 7 1 5
3J16_L 0.31 0.33 0.30 7 1136 16 3 13 0 14
3JYV_7 -0.02 0.00 0.00 0 1089 22 4 18 0 20
3JYX_3 0.77 0.80 0.75 12 2362 27 0 4 23 3
3JYX_4 0.61 0.83 0.45 10 4734 31 10 2 19 2
3KTW_C - 0.96 0.96 0.96 24 1755 9 0 1 8 1
3LA5_A 0.91 0.84 1.00 21 933 0 0 0 0 4
3NDB_M - 1.00 1.00 1.00 44 3670 4 0 0 4 0
3NKB_B - 0.69 0.74 0.67 14 714 7 0 7 0 5
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.85 0.78 0.94 29 2247 6 0 2 4 8
3O58_2 0.87 0.87 0.87 27 2723 12 0 4 8 4
3O58_3 0.26 0.36 0.19 8 4722 35 16 18 1 14
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.81 0.80 0.82 40 4791 11 3 6 2 10
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.77 0.76 0.79 22 1505 6 4 2 0 7
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.49 0.55 0.46 12 1250 14 3 11 0 10
3UZL_B 0.49 0.50 0.50 8 1277 15 0 8 7 8
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
4A1C_2 0.19 0.25 0.15 5 4483 42 12 16 14 15
4A1C_3 0.88 0.86 0.89 32 2727 7 0 4 3 5
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.60 0.59 0.63 17 1410 11 4 6 1 12
4ENB_A 1.00 1.00 1.00 15 457 0 0 0 0 0
4ENC_A 0.97 1.00 0.94 15 480 1 1 0 0 0

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Performance of RNAsubopt - scored lower in this pairwise comparison

1. Total counts & total scores for RNAsubopt

Total Base Pair Counts
Total TP 1542
Total TN 149912
Total FP 846
Total FP CONTRA 185
Total FP INCONS 396
Total FP COMP 265
Total FN 494
Total Scores
MCC 0.738
Average MCC ± 95% Confidence Intervals 0.770 ± 0.057
Sensitivity 0.757
Positive Predictive Value 0.726
Nr of predictions 118

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2. Individual counts for RNAsubopt [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 0.95 0.91 1.00 10 176 0 0 0 0 1
2K63_A - 0.94 0.89 1.00 8 160 0 0 0 0 1
2K66_A - 1.00 1.00 1.00 9 80 0 0 0 0 0
2KD8_A - 0.87 0.78 1.00 7 101 0 0 0 0 2
2KDQ_B 1.00 1.00 1.00 10 164 0 0 0 0 0
2KE6_A 0.88 0.89 0.89 16 449 3 0 2 1 2
2KEZ_A - 1.00 1.00 1.00 8 94 0 0 0 0 0
2KFC_A - -0.03 0.00 0.00 0 229 7 1 6 0 8
2KGP_A - 1.00 1.00 1.00 9 112 0 0 0 0 0
2KMJ_A - 1.00 1.00 1.00 11 157 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.62 0.61 0.64 14 2002 15 5 3 7 9
2KU0_A - 0.96 0.92 1.00 11 281 0 0 0 0 1
2KUR_A 0.89 0.89 0.89 17 448 2 0 2 0 2
2KUU_A 0.88 0.89 0.89 16 429 3 0 2 1 2
2KUV_A 0.89 0.89 0.89 17 420 2 0 2 0 2
2KUW_A 0.94 0.94 0.94 17 452 2 0 1 1 1
2KX8_A 0.97 0.94 1.00 15 356 0 0 0 0 1
2KXM_A - 1.00 1.00 1.00 8 141 0 0 0 0 0
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_A 0.95 0.91 1.00 21 742 0 0 0 0 2
2L1F_B 0.96 0.92 1.00 22 769 0 0 0 0 2
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 0.96 0.93 1.00 13 226 0 0 0 0 1
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A -0.03 0.00 0.00 0 513 15 3 12 0 18
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 0.85 0.86 0.83 25 2410 14 0 5 9 4
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.95 0.91 1.00 10 197 1 0 0 1 1
2RP0_A - 0.84 0.71 1.00 5 111 0 0 0 0 2
2RPT_A - 1.00 1.00 1.00 7 65 0 0 0 0 0
2RRC_A - 1.00 1.00 1.00 7 68 0 0 0 0 0
2WRQ_Y 1.00 1.00 1.00 9 1143 13 0 0 13 0
2WW9_D - 0.09 0.10 0.11 1 732 13 2 6 5 9
2WW9_E - -0.03 0.00 0.00 0 168 5 0 4 1 5
2WW9_F - 0.52 0.63 0.50 5 102 5 1 4 0 3
2WWQ_V 1.00 1.00 1.00 19 1185 5 0 0 5 0
2XKV_B 0.64 0.73 0.57 8 1821 25 0 6 19 3
2XQD_Y 0.83 0.81 0.85 17 1109 4 0 3 1 4
2XXA_G 0.49 0.49 0.52 17 2012 17 1 15 1 18
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_X - 1.00 1.00 1.00 7 537 4 0 0 4 0
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
2ZY6_A - 0.66 0.78 0.58 7 264 5 3 2 0 2
2ZZM_B 0.29 0.33 0.26 5 1339 22 5 9 8 10
2ZZN_D 0.52 0.55 0.52 12 961 11 3 8 0 10
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3A3A_A 0.97 0.93 1.00 28 1472 0 0 0 0 2
3ADB_C - 0.98 0.97 1.00 32 1787 0 0 0 0 1
3AKZ_H 0.43 0.45 0.43 9 1106 14 3 9 2 11
3AM1_B - 1.00 1.00 1.00 29 1437 0 0 0 0 0
3AMU_B 0.81 0.79 0.83 15 1139 6 0 3 3 4
3DW4_A - -0.03 0.00 0.00 0 136 6 1 3 2 6
3G4S_9 0.41 0.46 0.38 12 2704 25 7 13 5 14
3GCA_A - 0.84 0.71 1.00 5 153 0 0 0 0 2
3GX2_A 0.53 0.54 0.54 15 1421 14 4 9 1 13
3HAY_E - 1.00 1.00 1.00 14 910 4 0 0 4 0
3HJW_D - 0.97 0.94 1.00 15 594 2 0 0 2 1
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3ID5_D - 0.54 0.60 0.50 3 223 5 1 2 2 2
3IVN_B 0.88 0.78 1.00 18 885 0 0 0 0 5
3IWN_A 0.69 0.68 0.70 19 1445 9 1 7 1 9
3IYQ_A 0.28 0.39 0.20 20 22342 96 41 37 18 31
3IZ4_A 0.56 0.60 0.53 57 25429 54 26 24 4 38
3IZF_C 0.89 0.91 0.86 32 2603 9 0 5 4 3
3J0L_h - 0.87 0.81 0.93 26 2112 4 1 1 2 6
3J0L_g - -0.01 0.00 0.00 0 173 5 1 2 2 2
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_a - 0.35 0.36 0.36 4 400 8 3 4 1 7
3J0L_2 - 0.28 0.31 0.28 8 2221 25 2 19 4 18
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_7 - -0.02 0.00 0.00 0 507 12 2 10 0 10
3J16_L 0.49 0.52 0.48 11 1136 12 3 9 0 10
3JYV_7 -0.02 0.00 0.00 0 1090 21 4 17 0 20
3JYX_3 0.77 0.80 0.75 12 2362 26 0 4 22 3
3JYX_4 0.34 0.58 0.21 7 4722 38 20 7 11 5
3KTW_C - 1.00 1.00 1.00 25 1755 8 0 0 8 0
3LA5_A 0.91 0.84 1.00 21 933 0 0 0 0 4
3NDB_M - 0.93 0.93 0.93 41 3670 9 0 3 6 3
3NKB_B - 0.66 0.68 0.65 13 715 7 0 7 0 6
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.84 0.78 0.91 29 2246 8 0 3 5 8
3O58_2 0.87 0.90 0.85 28 2721 13 0 5 8 3
3O58_3 0.41 0.50 0.34 11 4732 35 6 15 14 11
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.90 0.90 0.90 45 4790 7 2 3 2 5
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.73 0.72 0.75 21 1505 7 4 3 0 8
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.49 0.55 0.46 12 1250 14 3 11 0 10
3UZL_B 0.49 0.50 0.50 8 1277 15 0 8 7 8
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
4A1C_2 0.18 0.25 0.14 5 4481 43 13 17 13 15
4A1C_3 0.85 0.84 0.86 31 2727 8 0 5 3 6
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.62 0.62 0.64 18 1409 11 4 6 1 11
4ENB_A 0.85 0.73 1.00 11 461 2 0 0 2 4
4ENC_A 0.36 0.33 0.42 5 484 7 0 7 0 10

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.