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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of CentroidHomfold‑LAST - scored higher in this pairwise comparison

  4. Performance of NanoFolder - scored lower in this pairwise comparison

  5. Compile and download dataset for CentroidHomfold‑LAST & NanoFolder [.zip] - may take several seconds...


Overview

Metric CentroidHomfold‑LAST NanoFolder
MCC 0.760 > 0.335
Average MCC ± 95% Confidence Intervals 0.741 ± 0.056 > 0.389 ± 0.054
Sensitivity 0.686 > 0.384
Positive Predictive Value 0.844 > 0.301
Total TP 1900 > 1064
Total TN 414748 > 413459
Total FP 451 < 2599
Total FP CONTRA 35 < 475
Total FP INCONS 315 < 2000
Total FP COMP 101 < 124
Total FN 871 < 1707
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of CentroidHomfold-LAST and NanoFolder. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for CentroidHomfold‑LAST and NanoFolder).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for CentroidHomfold‑LAST and NanoFolder).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for CentroidHomfold-LAST and NanoFolder. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for CentroidHomfold‑LAST and NanoFolder).

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Performance of CentroidHomfold‑LAST - scored higher in this pairwise comparison

1. Total counts & total scores for CentroidHomfold‑LAST

Total Base Pair Counts
Total TP 1900
Total TN 414748
Total FP 451
Total FP CONTRA 35
Total FP INCONS 315
Total FP COMP 101
Total FN 871
Total Scores
MCC 0.760
Average MCC ± 95% Confidence Intervals 0.741 ± 0.056
Sensitivity 0.686
Positive Predictive Value 0.844
Nr of predictions 117

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2. Individual counts for CentroidHomfold‑LAST [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
CRW_01535 0.79 0.78 0.81 29 7104 12 0 7 5 8
CRW_01539 0.78 0.76 0.80 28 7105 13 0 7 6 9
CRW_01563 0.86 0.81 0.91 30 6870 4 0 3 1 7
CRW_01583 0.84 0.78 0.91 29 7108 4 0 3 1 8
CRW_01590 0.87 0.84 0.91 31 7106 4 0 3 1 6
CRW_01593 0.79 0.78 0.81 29 7104 11 0 7 4 8
CRW_01595 0.78 0.76 0.80 28 8480 8 0 7 1 9
CRW_01603 0.86 0.81 0.91 30 7107 4 0 3 1 7
CRW_01617 0.80 0.78 0.83 29 7105 12 0 6 6 8
CRW_01625 0.80 0.78 0.83 29 7105 7 0 6 1 8
PDB_00011 - 0.91 0.83 1.00 10 266 0 0 0 0 2
PDB_00017 - 0.77 0.60 1.00 9 694 0 0 0 0 6
PDB_00036 - 0.00 0.00 0.00 0 3486 0 0 0 0 27
PDB_00070 0.71 0.58 0.88 14 2834 2 0 2 0 10
PDB_00113 - 0.94 0.89 1.00 8 223 0 0 0 0 1
PDB_00119 - 0.95 0.90 1.00 9 244 0 0 0 0 1
PDB_00136 - 0.73 0.54 1.00 14 2401 0 0 0 0 12
PDB_00137 - 0.87 0.77 1.00 10 396 0 0 0 0 3
PDB_00160 - 0.94 0.89 1.00 8 268 0 0 0 0 1
PDB_00178 - 0.52 0.27 1.00 3 525 0 0 0 0 8
PDB_00221 - 0.74 0.56 1.00 5 248 0 0 0 0 4
PDB_00312 - 1.00 1.00 1.00 9 426 0 0 0 0 0
PDB_00326 - 0.80 0.68 0.95 19 3721 1 0 1 0 9
PDB_00341 - 0.79 0.63 1.00 10 693 0 0 0 0 6
PDB_00349 - -0.01 0.00 0.00 0 936 10 0 10 0 14
PDB_00417 - 0.12 0.11 0.14 6 14154 36 4 32 0 50
PDB_00430 0.92 0.84 1.00 21 3219 0 0 0 0 4
PDB_00527 - 0.00 0.00 0.00 0 300 0 0 0 0 5
PDB_00565 - 0.41 0.22 0.79 11 8114 4 0 3 1 39
PDB_00570 - 0.90 0.82 1.00 18 2260 0 0 0 0 4
PDB_00571 0.87 0.80 0.95 20 3300 1 1 0 0 5
PDB_00573 - 0.48 0.41 0.56 14 6645 14 0 11 3 20
PDB_00584 - 0.58 0.47 0.73 8 1474 3 0 3 0 9
PDB_00698 - 0.94 0.89 1.00 17 844 0 0 0 0 2
PDB_00725 - 0.93 0.87 1.00 13 582 0 0 0 0 2
PDB_00734 - 0.83 0.70 1.00 7 224 0 0 0 0 3
PDB_00823 - -0.03 0.00 0.00 0 186 4 0 4 0 9
PDB_00828 0.84 0.70 1.00 19 2466 0 0 0 0 8
PDB_00851 - 0.44 0.39 0.52 16 4722 15 0 15 0 25
PDB_00890 - -0.02 0.00 0.00 0 690 13 2 11 0 14
PDB_00923 - 1.00 1.00 1.00 6 184 0 0 0 0 0
PDB_00994 - 0.28 0.17 0.47 7 10570 10 0 8 2 35
PDB_01009 0.95 0.90 1.00 19 2466 2 0 0 2 2
PDB_01051 0.96 0.92 1.00 12 891 4 0 0 4 1
PDB_01074 - -0.01 0.00 0.00 0 2746 29 3 26 0 25
PDB_01130 - 0.00 0.00 0.00 0 1770 0 0 0 0 20
PDB_01199 - 0.20 0.04 1.00 1 2144 0 0 0 0 23
PDB_01236 0.84 0.72 0.98 39 11588 2 0 1 1 15
PDB_01275 - 0.59 0.35 1.00 25 18311 2 0 0 2 46
RFA_00416 1.00 1.00 1.00 15 1470 2 0 0 2 0
RFA_00434 1.00 1.00 1.00 15 1416 2 0 0 2 0
RFA_00436 1.00 1.00 1.00 15 1416 2 0 0 2 0
RFA_00442 1.00 1.00 1.00 15 1416 2 0 0 2 0
RFA_00446 0.89 0.80 1.00 12 1473 2 0 0 2 3
RFA_00604 0.74 0.63 0.88 22 13505 8 3 0 5 13
RFA_00664 -0.01 0.00 0.00 0 983 7 0 7 0 14
RFA_00674 0.74 0.71 0.77 10 1115 4 0 3 1 4
RFA_00675 0.35 0.29 0.44 4 981 5 0 5 0 10
RFA_00677 0.74 0.71 0.77 10 977 5 0 3 2 4
RFA_00684 0.84 0.71 1.00 10 980 1 0 0 1 4
RFA_00703 0.43 0.43 0.43 6 4264 21 1 7 13 8
RFA_00716 -0.01 0.00 0.00 0 939 7 0 7 0 14
RFA_00717 0.80 0.64 1.00 9 894 0 0 0 0 5
RFA_00730 1.00 1.00 1.00 12 891 1 0 0 1 0
RFA_00763 1.00 1.00 1.00 12 891 1 0 0 1 0
RFA_00765 1.00 1.00 1.00 12 891 1 0 0 1 0
RFA_00770 0.58 0.39 0.88 7 2008 1 0 1 0 11
RFA_00773 0.55 0.56 0.56 10 1935 8 3 5 0 8
RFA_00808 0.75 0.56 1.00 9 2007 0 0 0 0 7
SPR_00030 - 0.49 0.24 1.00 5 2480 0 0 0 0 16
SPR_00066 1.00 1.00 1.00 21 2829 0 0 0 0 0
SPR_00228 1.00 1.00 1.00 20 3808 3 0 0 3 0
SPR_00314 1.00 1.00 1.00 21 2905 1 0 0 1 0
SPR_00331 1.00 1.00 1.00 20 2755 0 0 0 0 0
SPR_00333 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_00353 1.00 1.00 1.00 21 2829 1 0 0 1 0
SPR_00398 - 0.07 0.08 0.07 1 2197 13 2 11 0 12
SPR_00452 1.00 1.00 1.00 21 2829 0 0 0 0 0
SPR_00456 1.00 1.00 1.00 21 2829 1 0 0 1 0
SPR_00462 0.95 0.95 0.95 18 2396 1 0 1 0 1
SPR_00476 0.71 0.71 0.71 15 2754 6 1 5 0 6
SPR_00496 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_00497 0.71 0.71 0.71 15 2754 6 1 5 0 6
SPR_00502 1.00 1.00 1.00 21 2905 0 0 0 0 0
SPR_00509 1.00 1.00 1.00 20 2906 0 0 0 0 0
SPR_00512 1.00 1.00 1.00 20 2906 0 0 0 0 0
SPR_00542 0.88 0.86 0.90 18 3550 2 0 2 0 3
SPR_00557 1.00 1.00 1.00 21 2829 0 0 0 0 0
SPR_00832 - 0.00 0.00 0.00 0 1653 0 0 0 0 15
SPR_00868 1.00 1.00 1.00 21 2905 1 0 0 1 0
SPR_00932 0.82 0.76 0.89 16 2832 3 0 2 1 5
SPR_00957 1.00 1.00 1.00 21 2982 1 0 0 1 0
SPR_01040 1.00 1.00 1.00 21 2754 1 0 0 1 0
SPR_01143 0.95 0.95 0.95 20 2535 1 0 1 0 1
SRP_00005 - 0.75 0.75 0.75 18 3981 6 0 6 0 6
SRP_00038 0.52 0.48 0.57 16 5022 12 0 12 0 17
SRP_00046 0.95 0.94 0.97 30 4722 2 0 1 1 2
SRP_00138 - 1.00 1.00 1.00 27 3978 5 0 0 5 0
SRP_00170 0.94 0.96 0.92 24 4069 3 1 1 1 1
SRP_00193 - 0.81 0.78 0.85 28 6870 5 1 4 0 8
SRP_00194 0.74 0.76 0.73 16 3218 6 0 6 0 5
SRP_00200 0.81 0.78 0.85 28 6870 5 1 4 0 8
SRP_00216 0.91 0.89 0.92 24 3377 3 1 1 1 3
SRP_00229 0.37 0.37 0.37 10 5226 17 5 12 0 17
SRP_00233 0.95 0.94 0.97 31 5119 1 0 1 0 2
SRP_00243 0.82 0.68 1.00 21 5130 1 0 0 1 10
SRP_00244 0.83 0.74 0.94 29 6872 3 1 1 1 10
SRP_00266 0.59 0.55 0.64 16 4631 10 1 8 1 13
SRP_00269 0.95 0.91 1.00 30 5020 0 0 0 0 3
SRP_00273 0.82 0.75 0.91 30 6753 4 1 2 1 10
SRP_00285 0.95 0.93 0.97 28 3712 1 0 1 0 2
SRP_00303 0.76 0.75 0.77 24 6639 8 1 6 1 8
SRP_00333 0.94 0.91 0.97 31 5119 1 0 1 0 3
SRP_00338 0.96 0.94 0.97 34 5425 1 0 1 0 2
SRP_00342 0.66 0.44 1.00 11 3994 0 0 0 0 14
SRP_00358 0.90 0.86 0.94 30 6296 3 1 1 1 5
SRP_00383 0.87 0.83 0.91 20 3218 2 0 2 0 4

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Performance of NanoFolder - scored lower in this pairwise comparison

1. Total counts & total scores for NanoFolder

Total Base Pair Counts
Total TP 1064
Total TN 413459
Total FP 2599
Total FP CONTRA 475
Total FP INCONS 2000
Total FP COMP 124
Total FN 1707
Total Scores
MCC 0.335
Average MCC ± 95% Confidence Intervals 0.389 ± 0.054
Sensitivity 0.384
Positive Predictive Value 0.301
Nr of predictions 117

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2. Individual counts for NanoFolder [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
CRW_01535 0.20 0.24 0.17 9 7088 43 7 36 0 28
CRW_01539 0.10 0.11 0.10 4 7099 40 1 36 3 33
CRW_01563 0.11 0.14 0.11 5 6856 43 5 37 1 32
CRW_01583 0.36 0.41 0.33 15 7095 31 8 22 1 22
CRW_01590 -0.01 0.00 0.00 0 7093 50 7 40 3 37
CRW_01593 0.32 0.38 0.29 14 7091 36 5 30 1 23
CRW_01595 -0.01 0.00 0.00 0 8459 57 10 46 1 37
CRW_01603 0.41 0.46 0.38 17 7095 29 6 22 1 20
CRW_01617 0.20 0.24 0.17 9 7087 45 7 37 1 28
CRW_01625 0.43 0.49 0.38 18 7093 31 7 22 2 19
PDB_00011 - 0.96 0.92 1.00 11 265 0 0 0 0 1
PDB_00017 - 0.66 0.67 0.67 10 688 5 1 4 0 5
PDB_00036 - -0.01 0.00 0.00 0 3457 29 1 28 0 27
PDB_00070 0.25 0.29 0.23 7 2819 24 4 20 0 17
PDB_00113 - 0.94 0.89 1.00 8 223 0 0 0 0 1
PDB_00119 - 0.95 0.90 1.00 9 244 0 0 0 0 1
PDB_00136 - 0.70 0.73 0.68 19 2387 9 1 8 0 7
PDB_00137 - 0.87 0.77 1.00 10 396 0 0 0 0 3
PDB_00160 - 0.89 0.89 0.89 8 267 2 0 1 1 1
PDB_00178 - -0.02 0.00 0.00 0 517 11 1 10 0 11
PDB_00221 - 0.94 0.89 1.00 8 245 0 0 0 0 1
PDB_00312 - 1.00 1.00 1.00 9 426 1 0 0 1 0
PDB_00326 - 0.58 0.64 0.53 18 3707 16 4 12 0 10
PDB_00341 - 0.47 0.44 0.54 7 690 6 0 6 0 9
PDB_00349 - 0.10 0.14 0.10 2 926 18 0 18 0 12
PDB_00417 - 0.00 0.00 0.00 0 14138 58 2 56 0 56
PDB_00430 0.38 0.44 0.34 11 3208 21 6 15 0 14
PDB_00527 - -0.02 0.00 0.00 0 292 8 2 6 0 5
PDB_00565 - 0.24 0.24 0.24 12 8079 37 1 36 0 38
PDB_00570 - -0.01 0.00 0.00 0 2257 21 0 21 0 22
PDB_00571 -0.01 0.00 0.00 0 3286 36 6 29 1 25
PDB_00573 - -0.01 0.00 0.00 0 6622 48 5 43 0 34
PDB_00584 - 0.47 0.53 0.43 9 1464 12 1 11 0 8
PDB_00698 - 0.97 0.95 1.00 18 843 0 0 0 0 1
PDB_00725 - 0.82 0.80 0.86 12 581 2 0 2 0 3
PDB_00734 - 0.83 0.70 1.00 7 224 0 0 0 0 3
PDB_00823 - -0.04 0.00 0.00 0 183 7 0 7 0 9
PDB_00828 0.30 0.33 0.28 9 2453 23 0 23 0 18
PDB_00851 - 0.16 0.17 0.17 7 4711 35 3 32 0 34
PDB_00890 - -0.02 0.00 0.00 0 690 13 2 11 0 14
PDB_00923 - 1.00 1.00 1.00 6 184 2 0 0 2 0
PDB_00994 - 0.40 0.50 0.33 21 10521 44 13 30 1 21
PDB_01009 0.27 0.33 0.23 7 2455 25 3 20 2 14
PDB_01051 -0.02 0.00 0.00 0 885 18 1 17 0 13
PDB_01074 - -0.01 0.00 0.00 0 2747 28 3 25 0 25
PDB_01130 - -0.01 0.00 0.00 0 1742 28 5 23 0 20
PDB_01199 - -0.01 0.00 0.00 0 2116 29 2 27 0 24
PDB_01236 0.31 0.33 0.30 18 11568 43 3 39 1 36
PDB_01275 - 0.04 0.04 0.04 3 18258 75 6 69 0 68
RFA_00416 0.94 1.00 0.88 15 1468 6 2 0 4 0
RFA_00434 0.84 1.00 0.71 15 1410 8 6 0 2 0
RFA_00436 0.86 1.00 0.75 15 1411 7 5 0 2 0
RFA_00442 0.94 1.00 0.88 15 1414 4 2 0 2 0
RFA_00446 0.85 0.93 0.78 14 1467 7 3 1 3 1
RFA_00604 0.42 0.54 0.33 19 13472 53 14 25 14 16
RFA_00664 -0.01 0.00 0.00 0 978 13 0 12 1 14
RFA_00674 0.12 0.14 0.13 2 1112 15 0 14 1 12
RFA_00675 0.28 0.29 0.29 4 976 10 1 9 0 10
RFA_00677 0.62 0.64 0.60 9 975 10 0 6 4 5
RFA_00684 0.74 0.71 0.77 10 977 5 2 1 2 4
RFA_00703 0.26 0.36 0.19 5 4252 32 5 16 11 9
RFA_00716 -0.02 0.00 0.00 0 929 17 0 17 0 14
RFA_00717 0.64 0.64 0.64 9 889 5 2 3 0 5
RFA_00730 0.38 0.42 0.36 5 889 10 3 6 1 7
RFA_00763 0.38 0.42 0.36 5 889 10 3 6 1 7
RFA_00765 0.39 0.42 0.38 5 890 9 2 6 1 7
RFA_00770 -0.01 0.00 0.00 0 1996 24 0 20 4 18
RFA_00773 0.93 1.00 0.86 18 1932 9 3 0 6 0
RFA_00808 0.83 1.00 0.70 16 1993 7 7 0 0 0
SPR_00030 - 0.47 0.52 0.42 11 2459 16 2 13 1 10
SPR_00066 0.47 0.57 0.39 12 2819 20 4 15 1 9
SPR_00228 0.44 0.60 0.32 12 3791 26 15 10 1 8
SPR_00314 0.47 0.57 0.39 12 2895 21 9 10 2 9
SPR_00331 0.48 0.60 0.39 12 2744 19 6 13 0 8
SPR_00333 0.25 0.33 0.21 7 2741 27 10 17 0 14
SPR_00353 0.64 0.81 0.52 17 2817 16 8 8 0 4
SPR_00398 - -0.01 0.00 0.00 0 2185 28 10 16 2 13
SPR_00452 0.27 0.33 0.23 7 2820 24 7 16 1 14
SPR_00456 0.49 0.57 0.43 12 2822 19 5 11 3 9
SPR_00462 0.65 0.74 0.58 14 2391 11 4 6 1 5
SPR_00476 0.47 0.52 0.42 11 2749 17 4 11 2 10
SPR_00496 0.47 0.57 0.40 12 2745 22 7 11 4 9
SPR_00497 0.27 0.33 0.23 7 2745 23 5 18 0 14
SPR_00502 0.27 0.33 0.23 7 2896 26 2 21 3 14
SPR_00509 0.23 0.30 0.19 6 2895 25 8 17 0 14
SPR_00512 0.61 0.75 0.50 15 2896 15 6 9 0 5
SPR_00542 0.26 0.33 0.22 7 3538 28 4 21 3 14
SPR_00557 0.42 0.52 0.34 11 2818 22 8 13 1 10
SPR_00832 - 0.53 0.67 0.43 10 1630 13 5 8 0 5
SPR_00868 0.49 0.57 0.43 12 2898 19 5 11 3 9
SPR_00932 0.43 0.52 0.35 11 2819 21 7 13 1 10
SPR_00957 0.63 0.76 0.53 16 2973 15 7 7 1 5
SPR_01040 0.15 0.19 0.13 4 2744 27 4 23 0 17
SPR_01143 0.41 0.48 0.36 10 2528 18 8 10 0 11
SRP_00005 - -0.01 0.00 0.00 0 3966 39 6 33 0 24
SRP_00038 0.23 0.27 0.20 9 5005 36 1 35 0 24
SRP_00046 0.33 0.38 0.29 12 4712 30 3 26 1 20
SRP_00138 - 0.44 0.52 0.39 14 3969 24 8 14 2 13
SRP_00170 0.29 0.36 0.25 9 4059 28 4 23 1 16
SRP_00193 - 0.16 0.19 0.15 7 6855 43 4 37 2 29
SRP_00194 0.27 0.33 0.23 7 3209 27 5 19 3 14
SRP_00200 0.41 0.47 0.35 17 6855 31 4 27 0 19
SRP_00216 0.44 0.48 0.41 13 3371 19 4 15 0 14
SRP_00229 0.42 0.52 0.35 14 5213 26 8 18 0 13
SRP_00233 0.25 0.30 0.22 10 5106 35 4 31 0 23
SRP_00243 0.47 0.55 0.40 17 5109 25 7 18 0 14
SRP_00244 0.38 0.44 0.34 17 6853 33 2 31 0 22
SRP_00266 0.55 0.66 0.46 19 4615 22 8 14 0 10
SRP_00269 0.46 0.52 0.43 17 5010 24 6 17 1 16
SRP_00273 0.36 0.40 0.33 16 6737 34 6 27 1 24
SRP_00285 0.38 0.43 0.35 13 3704 24 6 18 0 17
SRP_00303 0.36 0.41 0.33 13 6630 27 3 24 0 19
SRP_00333 0.20 0.24 0.19 8 5108 35 3 32 0 26
SRP_00338 0.52 0.56 0.49 20 5419 22 4 17 1 16
SRP_00342 0.20 0.24 0.17 6 3970 29 7 22 0 19
SRP_00358 0.32 0.37 0.28 13 6282 33 4 29 0 22
SRP_00383 -0.01 0.00 0.00 0 3205 35 9 26 0 24

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.