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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of UNAFold - scored higher in this pairwise comparison

  4. Performance of NanoFolder - scored lower in this pairwise comparison

  5. Compile and download dataset for UNAFold & NanoFolder [.zip] - may take several seconds...


Overview

Metric UNAFold NanoFolder
MCC 0.670 > 0.335
Average MCC ± 95% Confidence Intervals 0.668 ± 0.056 > 0.389 ± 0.054
Sensitivity 0.654 > 0.384
Positive Predictive Value 0.691 > 0.301
Total TP 1813 > 1064
Total TN 414374 > 413459
Total FP 947 < 2599
Total FP CONTRA 113 < 475
Total FP INCONS 698 < 2000
Total FP COMP 136 > 124
Total FN 958 < 1707
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of UNAFold and NanoFolder. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for UNAFold and NanoFolder).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for UNAFold and NanoFolder).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for UNAFold and NanoFolder. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for UNAFold and NanoFolder).

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Performance of UNAFold - scored higher in this pairwise comparison

1. Total counts & total scores for UNAFold

Total Base Pair Counts
Total TP 1813
Total TN 414374
Total FP 947
Total FP CONTRA 113
Total FP INCONS 698
Total FP COMP 136
Total FN 958
Total Scores
MCC 0.670
Average MCC ± 95% Confidence Intervals 0.668 ± 0.056
Sensitivity 0.654
Positive Predictive Value 0.691
Nr of predictions 117

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2. Individual counts for UNAFold [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
CRW_01535 0.83 0.76 0.90 28 7109 7 0 3 4 9
CRW_01539 0.81 0.73 0.90 27 7110 9 0 3 6 10
CRW_01563 0.61 0.54 0.69 20 6874 10 2 7 1 17
CRW_01583 0.80 0.76 0.85 28 7107 9 0 5 4 9
CRW_01590 0.29 0.30 0.29 11 7102 27 6 21 0 26
CRW_01593 0.80 0.76 0.85 28 7107 10 0 5 5 9
CRW_01595 0.74 0.70 0.79 26 8482 12 0 7 5 11
CRW_01603 0.84 0.78 0.91 29 7108 7 0 3 4 8
CRW_01617 0.90 0.86 0.94 32 7106 6 0 2 4 5
CRW_01625 0.88 0.86 0.89 32 7104 7 0 4 3 5
PDB_00011 - 0.91 0.83 1.00 10 266 0 0 0 0 2
PDB_00017 - 0.77 0.60 1.00 9 694 0 0 0 0 6
PDB_00036 - -0.01 0.00 0.00 0 3464 24 1 21 2 27
PDB_00070 0.93 0.88 1.00 21 2829 0 0 0 0 3
PDB_00113 - 0.94 0.89 1.00 8 223 0 0 0 0 1
PDB_00119 - 0.95 0.90 1.00 9 244 0 0 0 0 1
PDB_00136 - 0.90 0.85 0.96 22 2392 1 0 1 0 4
PDB_00137 - 0.87 0.77 1.00 10 396 0 0 0 0 3
PDB_00160 - 0.94 0.89 1.00 8 268 0 0 0 0 1
PDB_00178 - 0.90 0.82 1.00 9 519 1 0 0 1 2
PDB_00221 - 0.88 0.78 1.00 7 246 0 0 0 0 2
PDB_00312 - 1.00 1.00 1.00 9 426 0 0 0 0 0
PDB_00326 - 0.57 0.50 0.67 14 3720 7 0 7 0 14
PDB_00341 - 0.20 0.19 0.25 3 691 9 0 9 0 13
PDB_00349 - 0.64 0.64 0.64 9 932 5 0 5 0 5
PDB_00417 - 0.11 0.11 0.11 6 14143 47 5 42 0 50
PDB_00430 0.73 0.72 0.75 18 3216 6 3 3 0 7
PDB_00527 - -0.02 0.00 0.00 0 294 6 1 5 0 5
PDB_00565 - 0.76 0.68 0.85 34 8088 7 0 6 1 16
PDB_00570 - 0.90 0.82 1.00 18 2260 0 0 0 0 4
PDB_00571 0.21 0.20 0.24 5 3300 16 6 10 0 20
PDB_00573 - 0.75 0.71 0.80 24 6640 10 0 6 4 10
PDB_00584 - 0.58 0.47 0.73 8 1474 3 0 3 0 9
PDB_00698 - 0.94 0.89 1.00 17 844 0 0 0 0 2
PDB_00725 - 0.89 0.80 1.00 12 583 0 0 0 0 3
PDB_00734 - 0.83 0.70 1.00 7 224 0 0 0 0 3
PDB_00823 - -0.03 0.00 0.00 0 186 4 0 4 0 9
PDB_00828 0.88 0.78 1.00 21 2464 0 0 0 0 6
PDB_00851 - 0.45 0.39 0.53 16 4723 14 0 14 0 25
PDB_00890 - 1.00 1.00 1.00 14 689 0 0 0 0 0
PDB_00923 - 1.00 1.00 1.00 6 184 0 0 0 0 0
PDB_00994 - 0.11 0.12 0.11 5 10540 41 5 35 1 37
PDB_01009 0.52 0.57 0.48 12 2460 13 5 8 0 9
PDB_01051 0.88 0.85 0.92 11 891 5 0 1 4 2
PDB_01074 - -0.01 0.00 0.00 0 2746 29 3 26 0 25
PDB_01130 - -0.01 0.00 0.00 0 1746 24 6 18 0 20
PDB_01199 - -0.01 0.00 0.00 0 2127 18 0 18 0 24
PDB_01236 0.73 0.69 0.77 37 11580 14 0 11 3 17
PDB_01275 - 0.89 0.79 1.00 56 18280 4 0 0 4 15
RFA_00416 0.93 0.93 0.93 14 1470 4 0 1 3 1
RFA_00434 1.00 1.00 1.00 15 1416 2 0 0 2 0
RFA_00436 1.00 1.00 1.00 15 1416 2 0 0 2 0
RFA_00442 1.00 1.00 1.00 15 1416 2 0 0 2 0
RFA_00446 0.97 0.93 1.00 14 1471 2 0 0 2 1
RFA_00604 0.70 0.71 0.69 25 13494 22 2 9 11 10
RFA_00664 -0.01 0.00 0.00 0 980 11 0 10 1 14
RFA_00674 0.71 0.71 0.71 10 1114 5 0 4 1 4
RFA_00675 0.29 0.29 0.31 4 977 9 1 8 0 10
RFA_00677 0.42 0.43 0.43 6 976 9 0 8 1 8
RFA_00684 0.66 0.64 0.69 9 977 5 0 4 1 5
RFA_00703 0.67 0.64 0.69 9 4265 18 1 3 14 5
RFA_00716 -0.01 0.00 0.00 0 935 11 0 11 0 14
RFA_00717 0.64 0.64 0.64 9 889 5 0 5 0 5
RFA_00730 0.69 0.67 0.73 8 892 4 1 2 1 4
RFA_00763 1.00 1.00 1.00 12 891 0 0 0 0 0
RFA_00765 1.00 1.00 1.00 12 891 1 0 0 1 0
RFA_00770 0.61 0.56 0.67 10 2001 8 0 5 3 8
RFA_00773 0.71 0.56 0.91 10 1942 4 1 0 3 8
RFA_00808 -0.01 0.00 0.00 0 2001 15 2 13 0 16
SPR_00030 - 0.49 0.43 0.56 9 2469 7 0 7 0 12
SPR_00066 1.00 1.00 1.00 21 2829 0 0 0 0 0
SPR_00228 0.46 0.50 0.43 10 3805 15 5 8 2 10
SPR_00314 0.71 0.76 0.67 16 2902 11 0 8 3 5
SPR_00331 0.47 0.50 0.45 10 2753 12 4 8 0 10
SPR_00333 0.46 0.48 0.45 10 2753 12 4 8 0 11
SPR_00353 1.00 1.00 1.00 21 2829 0 0 0 0 0
SPR_00398 - -0.01 0.00 0.00 0 2193 20 2 16 2 13
SPR_00452 0.73 0.76 0.70 16 2827 7 3 4 0 5
SPR_00456 0.55 0.57 0.55 12 2828 11 2 8 1 9
SPR_00462 0.61 0.58 0.65 11 2398 6 3 3 0 8
SPR_00476 0.42 0.48 0.38 10 2749 16 3 13 0 11
SPR_00496 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_00497 0.42 0.48 0.38 10 2749 16 3 13 0 11
SPR_00502 0.56 0.57 0.55 12 2904 10 2 8 0 9
SPR_00509 1.00 1.00 1.00 20 2906 0 0 0 0 0
SPR_00512 1.00 1.00 1.00 20 2906 0 0 0 0 0
SPR_00542 0.28 0.33 0.25 7 3542 21 4 17 0 14
SPR_00557 0.40 0.43 0.39 9 2827 14 1 13 0 12
SPR_00832 - 0.64 0.67 0.63 10 1637 6 2 4 0 5
SPR_00868 0.58 0.57 0.60 12 2906 9 0 8 1 9
SPR_00932 0.54 0.57 0.52 12 2827 11 1 10 0 9
SPR_00957 0.78 0.76 0.80 16 2983 5 0 4 1 5
SPR_01040 0.83 0.81 0.85 17 2755 4 0 3 1 4
SPR_01143 0.32 0.33 0.32 7 2534 15 3 12 0 14
SRP_00005 - 0.83 0.83 0.83 20 3981 6 0 4 2 4
SRP_00038 0.94 0.94 0.94 31 5017 2 0 2 0 2
SRP_00046 0.97 0.97 0.97 31 4721 2 0 1 1 1
SRP_00138 - 1.00 1.00 1.00 27 3978 6 0 0 6 0
SRP_00170 0.55 0.56 0.54 14 4069 13 0 12 1 11
SRP_00193 - 0.67 0.67 0.69 24 6868 12 1 10 1 12
SRP_00194 0.77 0.81 0.74 17 3217 6 0 6 0 4
SRP_00200 0.80 0.81 0.81 29 6867 7 1 6 0 7
SRP_00216 0.93 0.93 0.93 25 3376 3 1 1 1 2
SRP_00229 0.43 0.44 0.41 12 5224 17 5 12 0 15
SRP_00233 0.97 0.94 1.00 31 5120 0 0 0 0 2
SRP_00243 0.97 0.94 1.00 29 5122 1 0 0 1 2
SRP_00244 0.89 0.85 0.94 33 6868 3 1 1 1 6
SRP_00266 0.72 0.72 0.72 21 4627 9 1 7 1 8
SRP_00269 0.59 0.61 0.57 20 5015 15 3 12 0 13
SRP_00273 0.79 0.80 0.78 32 6745 10 1 8 1 8
SRP_00285 0.98 0.97 1.00 29 3712 0 0 0 0 1
SRP_00303 0.69 0.72 0.66 23 6635 12 1 11 0 9
SRP_00333 0.86 0.85 0.88 29 5118 4 0 4 0 5
SRP_00338 0.97 0.97 0.97 35 5424 1 0 1 0 1
SRP_00342 0.55 0.56 0.54 14 3979 17 1 11 5 11
SRP_00358 0.93 0.91 0.94 32 6294 2 1 1 0 3
SRP_00383 0.12 0.13 0.13 3 3217 20 3 17 0 21

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Performance of NanoFolder - scored lower in this pairwise comparison

1. Total counts & total scores for NanoFolder

Total Base Pair Counts
Total TP 1064
Total TN 413459
Total FP 2599
Total FP CONTRA 475
Total FP INCONS 2000
Total FP COMP 124
Total FN 1707
Total Scores
MCC 0.335
Average MCC ± 95% Confidence Intervals 0.389 ± 0.054
Sensitivity 0.384
Positive Predictive Value 0.301
Nr of predictions 117

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2. Individual counts for NanoFolder [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
CRW_01535 0.20 0.24 0.17 9 7088 43 7 36 0 28
CRW_01539 0.10 0.11 0.10 4 7099 40 1 36 3 33
CRW_01563 0.11 0.14 0.11 5 6856 43 5 37 1 32
CRW_01583 0.36 0.41 0.33 15 7095 31 8 22 1 22
CRW_01590 -0.01 0.00 0.00 0 7093 50 7 40 3 37
CRW_01593 0.32 0.38 0.29 14 7091 36 5 30 1 23
CRW_01595 -0.01 0.00 0.00 0 8459 57 10 46 1 37
CRW_01603 0.41 0.46 0.38 17 7095 29 6 22 1 20
CRW_01617 0.20 0.24 0.17 9 7087 45 7 37 1 28
CRW_01625 0.43 0.49 0.38 18 7093 31 7 22 2 19
PDB_00011 - 0.96 0.92 1.00 11 265 0 0 0 0 1
PDB_00017 - 0.66 0.67 0.67 10 688 5 1 4 0 5
PDB_00036 - -0.01 0.00 0.00 0 3457 29 1 28 0 27
PDB_00070 0.25 0.29 0.23 7 2819 24 4 20 0 17
PDB_00113 - 0.94 0.89 1.00 8 223 0 0 0 0 1
PDB_00119 - 0.95 0.90 1.00 9 244 0 0 0 0 1
PDB_00136 - 0.70 0.73 0.68 19 2387 9 1 8 0 7
PDB_00137 - 0.87 0.77 1.00 10 396 0 0 0 0 3
PDB_00160 - 0.89 0.89 0.89 8 267 2 0 1 1 1
PDB_00178 - -0.02 0.00 0.00 0 517 11 1 10 0 11
PDB_00221 - 0.94 0.89 1.00 8 245 0 0 0 0 1
PDB_00312 - 1.00 1.00 1.00 9 426 1 0 0 1 0
PDB_00326 - 0.58 0.64 0.53 18 3707 16 4 12 0 10
PDB_00341 - 0.47 0.44 0.54 7 690 6 0 6 0 9
PDB_00349 - 0.10 0.14 0.10 2 926 18 0 18 0 12
PDB_00417 - 0.00 0.00 0.00 0 14138 58 2 56 0 56
PDB_00430 0.38 0.44 0.34 11 3208 21 6 15 0 14
PDB_00527 - -0.02 0.00 0.00 0 292 8 2 6 0 5
PDB_00565 - 0.24 0.24 0.24 12 8079 37 1 36 0 38
PDB_00570 - -0.01 0.00 0.00 0 2257 21 0 21 0 22
PDB_00571 -0.01 0.00 0.00 0 3286 36 6 29 1 25
PDB_00573 - -0.01 0.00 0.00 0 6622 48 5 43 0 34
PDB_00584 - 0.47 0.53 0.43 9 1464 12 1 11 0 8
PDB_00698 - 0.97 0.95 1.00 18 843 0 0 0 0 1
PDB_00725 - 0.82 0.80 0.86 12 581 2 0 2 0 3
PDB_00734 - 0.83 0.70 1.00 7 224 0 0 0 0 3
PDB_00823 - -0.04 0.00 0.00 0 183 7 0 7 0 9
PDB_00828 0.30 0.33 0.28 9 2453 23 0 23 0 18
PDB_00851 - 0.16 0.17 0.17 7 4711 35 3 32 0 34
PDB_00890 - -0.02 0.00 0.00 0 690 13 2 11 0 14
PDB_00923 - 1.00 1.00 1.00 6 184 2 0 0 2 0
PDB_00994 - 0.40 0.50 0.33 21 10521 44 13 30 1 21
PDB_01009 0.27 0.33 0.23 7 2455 25 3 20 2 14
PDB_01051 -0.02 0.00 0.00 0 885 18 1 17 0 13
PDB_01074 - -0.01 0.00 0.00 0 2747 28 3 25 0 25
PDB_01130 - -0.01 0.00 0.00 0 1742 28 5 23 0 20
PDB_01199 - -0.01 0.00 0.00 0 2116 29 2 27 0 24
PDB_01236 0.31 0.33 0.30 18 11568 43 3 39 1 36
PDB_01275 - 0.04 0.04 0.04 3 18258 75 6 69 0 68
RFA_00416 0.94 1.00 0.88 15 1468 6 2 0 4 0
RFA_00434 0.84 1.00 0.71 15 1410 8 6 0 2 0
RFA_00436 0.86 1.00 0.75 15 1411 7 5 0 2 0
RFA_00442 0.94 1.00 0.88 15 1414 4 2 0 2 0
RFA_00446 0.85 0.93 0.78 14 1467 7 3 1 3 1
RFA_00604 0.42 0.54 0.33 19 13472 53 14 25 14 16
RFA_00664 -0.01 0.00 0.00 0 978 13 0 12 1 14
RFA_00674 0.12 0.14 0.13 2 1112 15 0 14 1 12
RFA_00675 0.28 0.29 0.29 4 976 10 1 9 0 10
RFA_00677 0.62 0.64 0.60 9 975 10 0 6 4 5
RFA_00684 0.74 0.71 0.77 10 977 5 2 1 2 4
RFA_00703 0.26 0.36 0.19 5 4252 32 5 16 11 9
RFA_00716 -0.02 0.00 0.00 0 929 17 0 17 0 14
RFA_00717 0.64 0.64 0.64 9 889 5 2 3 0 5
RFA_00730 0.38 0.42 0.36 5 889 10 3 6 1 7
RFA_00763 0.38 0.42 0.36 5 889 10 3 6 1 7
RFA_00765 0.39 0.42 0.38 5 890 9 2 6 1 7
RFA_00770 -0.01 0.00 0.00 0 1996 24 0 20 4 18
RFA_00773 0.93 1.00 0.86 18 1932 9 3 0 6 0
RFA_00808 0.83 1.00 0.70 16 1993 7 7 0 0 0
SPR_00030 - 0.47 0.52 0.42 11 2459 16 2 13 1 10
SPR_00066 0.47 0.57 0.39 12 2819 20 4 15 1 9
SPR_00228 0.44 0.60 0.32 12 3791 26 15 10 1 8
SPR_00314 0.47 0.57 0.39 12 2895 21 9 10 2 9
SPR_00331 0.48 0.60 0.39 12 2744 19 6 13 0 8
SPR_00333 0.25 0.33 0.21 7 2741 27 10 17 0 14
SPR_00353 0.64 0.81 0.52 17 2817 16 8 8 0 4
SPR_00398 - -0.01 0.00 0.00 0 2185 28 10 16 2 13
SPR_00452 0.27 0.33 0.23 7 2820 24 7 16 1 14
SPR_00456 0.49 0.57 0.43 12 2822 19 5 11 3 9
SPR_00462 0.65 0.74 0.58 14 2391 11 4 6 1 5
SPR_00476 0.47 0.52 0.42 11 2749 17 4 11 2 10
SPR_00496 0.47 0.57 0.40 12 2745 22 7 11 4 9
SPR_00497 0.27 0.33 0.23 7 2745 23 5 18 0 14
SPR_00502 0.27 0.33 0.23 7 2896 26 2 21 3 14
SPR_00509 0.23 0.30 0.19 6 2895 25 8 17 0 14
SPR_00512 0.61 0.75 0.50 15 2896 15 6 9 0 5
SPR_00542 0.26 0.33 0.22 7 3538 28 4 21 3 14
SPR_00557 0.42 0.52 0.34 11 2818 22 8 13 1 10
SPR_00832 - 0.53 0.67 0.43 10 1630 13 5 8 0 5
SPR_00868 0.49 0.57 0.43 12 2898 19 5 11 3 9
SPR_00932 0.43 0.52 0.35 11 2819 21 7 13 1 10
SPR_00957 0.63 0.76 0.53 16 2973 15 7 7 1 5
SPR_01040 0.15 0.19 0.13 4 2744 27 4 23 0 17
SPR_01143 0.41 0.48 0.36 10 2528 18 8 10 0 11
SRP_00005 - -0.01 0.00 0.00 0 3966 39 6 33 0 24
SRP_00038 0.23 0.27 0.20 9 5005 36 1 35 0 24
SRP_00046 0.33 0.38 0.29 12 4712 30 3 26 1 20
SRP_00138 - 0.44 0.52 0.39 14 3969 24 8 14 2 13
SRP_00170 0.29 0.36 0.25 9 4059 28 4 23 1 16
SRP_00193 - 0.16 0.19 0.15 7 6855 43 4 37 2 29
SRP_00194 0.27 0.33 0.23 7 3209 27 5 19 3 14
SRP_00200 0.41 0.47 0.35 17 6855 31 4 27 0 19
SRP_00216 0.44 0.48 0.41 13 3371 19 4 15 0 14
SRP_00229 0.42 0.52 0.35 14 5213 26 8 18 0 13
SRP_00233 0.25 0.30 0.22 10 5106 35 4 31 0 23
SRP_00243 0.47 0.55 0.40 17 5109 25 7 18 0 14
SRP_00244 0.38 0.44 0.34 17 6853 33 2 31 0 22
SRP_00266 0.55 0.66 0.46 19 4615 22 8 14 0 10
SRP_00269 0.46 0.52 0.43 17 5010 24 6 17 1 16
SRP_00273 0.36 0.40 0.33 16 6737 34 6 27 1 24
SRP_00285 0.38 0.43 0.35 13 3704 24 6 18 0 17
SRP_00303 0.36 0.41 0.33 13 6630 27 3 24 0 19
SRP_00333 0.20 0.24 0.19 8 5108 35 3 32 0 26
SRP_00338 0.52 0.56 0.49 20 5419 22 4 17 1 16
SRP_00342 0.20 0.24 0.17 6 3970 29 7 22 0 19
SRP_00358 0.32 0.37 0.28 13 6282 33 4 29 0 22
SRP_00383 -0.01 0.00 0.00 0 3205 35 9 26 0 24

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.