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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of Vsfold5 - scored higher in this pairwise comparison

  4. Performance of NanoFolder - scored lower in this pairwise comparison

  5. Compile and download dataset for Vsfold5 & NanoFolder [.zip] - may take several seconds...


Overview

Metric Vsfold5 NanoFolder
MCC 0.417 > 0.335
Average MCC ± 95% Confidence Intervals 0.464 ± 0.069 > 0.389 ± 0.054
Sensitivity 0.389 > 0.384
Positive Predictive Value 0.455 > 0.301
Total TP 1077 > 1064
Total TN 414629 > 413459
Total FP 1360 < 2599
Total FP CONTRA 180 < 475
Total FP INCONS 1112 < 2000
Total FP COMP 68 < 124
Total FN 1694 < 1707
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of Vsfold5 and NanoFolder. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for Vsfold5 and NanoFolder).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for Vsfold5 and NanoFolder).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for Vsfold5 and NanoFolder. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for Vsfold5 and NanoFolder).

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Performance of Vsfold5 - scored higher in this pairwise comparison

1. Total counts & total scores for Vsfold5

Total Base Pair Counts
Total TP 1077
Total TN 414629
Total FP 1360
Total FP CONTRA 180
Total FP INCONS 1112
Total FP COMP 68
Total FN 1694
Total Scores
MCC 0.417
Average MCC ± 95% Confidence Intervals 0.464 ± 0.069
Sensitivity 0.389
Positive Predictive Value 0.455
Nr of predictions 117

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2. Individual counts for Vsfold5 [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
CRW_01535 0.33 0.35 0.33 13 7100 28 2 25 1 24
CRW_01539 0.00 0.00 0.00 0 7110 33 3 27 3 37
CRW_01563 0.44 0.38 0.52 14 6876 13 3 10 0 23
CRW_01583 0.30 0.27 0.33 10 7110 20 2 18 0 27
CRW_01590 -0.01 0.00 0.00 0 7103 37 8 29 0 37
CRW_01593 0.26 0.24 0.28 9 7108 23 4 19 0 28
CRW_01595 0.00 0.00 0.00 0 8476 39 2 37 0 37
CRW_01603 0.11 0.11 0.13 4 7108 28 2 26 0 33
CRW_01617 0.48 0.46 0.52 17 7107 16 4 12 0 20
CRW_01625 0.32 0.32 0.32 12 7103 25 3 22 0 25
PDB_00011 - -0.03 0.00 0.00 0 269 7 0 7 0 12
PDB_00017 - 0.77 0.60 1.00 9 694 0 0 0 0 6
PDB_00036 - -0.01 0.00 0.00 0 3466 26 0 20 6 27
PDB_00070 0.41 0.38 0.45 9 2830 12 0 11 1 15
PDB_00113 - 0.74 0.56 1.00 5 226 0 0 0 0 4
PDB_00119 - 0.95 0.90 1.00 9 244 0 0 0 0 1
PDB_00136 - 0.24 0.19 0.31 5 2399 11 0 11 0 21
PDB_00137 - 0.87 0.77 1.00 10 396 0 0 0 0 3
PDB_00160 - 0.94 0.89 1.00 8 268 0 0 0 0 1
PDB_00178 - 0.39 0.36 0.44 4 519 5 1 4 0 7
PDB_00221 - 0.62 0.56 0.71 5 246 2 0 2 0 4
PDB_00312 - 1.00 1.00 1.00 9 426 0 0 0 0 0
PDB_00326 - 0.56 0.46 0.68 13 3722 6 0 6 0 15
PDB_00341 - 0.55 0.44 0.70 7 693 3 0 3 0 9
PDB_00349 - 0.35 0.29 0.44 4 937 5 0 5 0 10
PDB_00417 - 0.12 0.11 0.14 6 14152 39 1 37 1 50
PDB_00430 0.73 0.76 0.70 19 3213 8 3 5 0 6
PDB_00527 - -0.02 0.00 0.00 0 294 6 1 5 0 5
PDB_00565 - 0.14 0.12 0.18 6 8094 28 1 27 0 44
PDB_00570 - 0.22 0.18 0.29 4 2264 10 1 9 0 18
PDB_00571 0.87 0.80 0.95 20 3300 1 1 0 0 5
PDB_00573 - 0.73 0.68 0.79 23 6641 8 0 6 2 11
PDB_00584 - 0.24 0.24 0.27 4 1470 11 0 11 0 13
PDB_00698 - -0.02 0.00 0.00 0 847 14 0 14 0 19
PDB_00725 - 0.85 0.73 1.00 11 584 0 0 0 0 4
PDB_00734 - 0.62 0.40 1.00 4 227 0 0 0 0 6
PDB_00823 - -0.03 0.00 0.00 0 186 4 0 4 0 9
PDB_00828 0.86 0.74 1.00 20 2465 0 0 0 0 7
PDB_00851 - 0.54 0.44 0.67 18 4726 9 0 9 0 23
PDB_00890 - 0.96 0.93 1.00 13 690 0 0 0 0 1
PDB_00923 - 1.00 1.00 1.00 6 184 0 0 0 0 0
PDB_00994 - 0.73 0.79 0.67 33 10536 19 9 7 3 9
PDB_01009 0.90 0.90 0.90 19 2464 3 0 2 1 2
PDB_01051 0.36 0.38 0.36 5 889 9 2 7 0 8
PDB_01074 - 0.34 0.32 0.38 8 2754 15 0 13 2 17
PDB_01130 - -0.01 0.00 0.00 0 1752 18 5 13 0 20
PDB_01199 - -0.01 0.00 0.00 0 2129 16 1 15 0 24
PDB_01236 0.50 0.43 0.59 23 11589 17 0 16 1 31
PDB_01275 - 0.26 0.23 0.30 16 18282 39 0 38 1 55
RFA_00416 0.93 0.93 0.93 14 1470 4 0 1 3 1
RFA_00434 0.97 0.93 1.00 14 1417 2 0 0 2 1
RFA_00436 0.97 0.93 1.00 14 1417 2 0 0 2 1
RFA_00442 0.97 0.93 1.00 14 1417 2 0 0 2 1
RFA_00446 0.97 0.93 1.00 14 1471 2 0 0 2 1
RFA_00604 0.48 0.49 0.49 17 13495 24 8 10 6 18
RFA_00664 -0.01 0.00 0.00 0 980 11 0 10 1 14
RFA_00674 -0.01 0.00 0.00 0 1117 11 0 11 0 14
RFA_00675 -0.01 0.00 0.00 0 981 9 1 8 0 14
RFA_00677 0.64 0.57 0.73 8 979 5 0 3 2 6
RFA_00684 0.75 0.57 1.00 8 982 1 0 0 1 6
RFA_00703 0.00 0.00 0.00 0 4271 20 0 7 13 14
RFA_00716 -0.01 0.00 0.00 0 936 10 0 10 0 14
RFA_00717 -0.01 0.00 0.00 0 894 9 0 9 0 14
RFA_00730 0.64 0.42 1.00 5 898 1 0 0 1 7
RFA_00763 0.64 0.42 1.00 5 898 0 0 0 0 7
RFA_00765 0.64 0.42 1.00 5 898 1 0 0 1 7
RFA_00770 0.65 0.56 0.77 10 2003 3 3 0 0 8
RFA_00773 -0.01 0.00 0.00 0 1936 19 1 16 2 18
RFA_00808 1.00 1.00 1.00 16 2000 0 0 0 0 0
SPR_00030 - 0.70 0.67 0.74 14 2466 5 0 5 0 7
SPR_00066 0.87 0.95 0.80 20 2825 5 5 0 0 1
SPR_00228 0.87 0.95 0.79 19 3804 5 5 0 0 1
SPR_00314 0.75 0.86 0.67 18 2899 9 7 2 0 3
SPR_00331 0.71 0.75 0.68 15 2753 7 6 1 0 5
SPR_00333 0.65 0.67 0.64 14 2753 8 2 6 0 7
SPR_00353 0.93 1.00 0.88 21 2826 3 3 0 0 0
SPR_00398 - -0.01 0.00 0.00 0 2194 19 2 15 2 13
SPR_00452 0.98 0.95 1.00 20 2830 0 0 0 0 1
SPR_00456 1.00 1.00 1.00 21 2829 1 0 0 1 0
SPR_00462 0.76 0.74 0.78 14 2397 5 3 1 1 5
SPR_00476 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_00496 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_00497 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_00502 1.00 1.00 1.00 21 2905 0 0 0 0 0
SPR_00509 1.00 1.00 1.00 20 2906 0 0 0 0 0
SPR_00512 0.97 0.95 1.00 19 2907 0 0 0 0 1
SPR_00542 0.53 0.57 0.50 12 3546 12 3 9 0 9
SPR_00557 0.53 0.57 0.50 12 2826 12 3 9 0 9
SPR_00832 - 0.27 0.27 0.29 4 1639 10 3 7 0 11
SPR_00868 0.67 0.71 0.63 15 2902 9 4 5 0 6
SPR_00932 0.98 0.95 1.00 20 2830 3 0 0 3 1
SPR_00957 1.00 1.00 1.00 21 2982 0 0 0 0 0
SPR_01040 1.00 1.00 1.00 21 2754 0 0 0 0 0
SPR_01143 0.47 0.52 0.42 11 2530 15 8 7 0 10
SRP_00005 - 0.17 0.17 0.19 4 3984 17 1 16 0 20
SRP_00038 0.28 0.27 0.29 9 5019 22 4 18 0 24
SRP_00046 -0.01 0.00 0.00 0 4733 20 0 20 0 32
SRP_00138 - -0.01 0.00 0.00 0 3980 25 6 19 0 27
SRP_00170 -0.01 0.00 0.00 0 4070 25 3 22 0 25
SRP_00193 - 0.00 0.00 0.00 0 6875 28 1 27 0 36
SRP_00194 0.32 0.33 0.32 7 3218 15 2 13 0 14
SRP_00200 0.00 0.00 0.00 0 6871 32 2 30 0 36
SRP_00216 0.48 0.44 0.52 12 3380 12 4 7 1 15
SRP_00229 0.15 0.15 0.17 4 5229 20 4 16 0 23
SRP_00233 0.63 0.61 0.67 20 5121 10 2 8 0 13
SRP_00243 0.45 0.39 0.52 12 5128 11 1 10 0 19
SRP_00244 0.00 0.00 0.00 0 6874 29 1 28 0 39
SRP_00266 0.32 0.31 0.33 9 4629 18 2 16 0 20
SRP_00269 0.23 0.21 0.27 7 5024 19 3 16 0 26
SRP_00273 -0.01 0.00 0.00 0 6754 32 2 30 0 40
SRP_00285 -0.01 0.00 0.00 0 3719 22 2 20 0 30
SRP_00303 0.00 0.00 0.00 0 6642 28 3 25 0 32
SRP_00333 -0.01 0.00 0.00 0 5127 24 2 22 0 34
SRP_00338 0.00 0.00 0.00 0 5460 0 0 0 0 36
SRP_00342 -0.01 0.00 0.00 0 3984 21 4 17 0 25
SRP_00358 0.00 0.00 0.00 0 6300 28 3 25 0 35
SRP_00383 -0.01 0.00 0.00 0 3215 25 2 23 0 24

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Performance of NanoFolder - scored lower in this pairwise comparison

1. Total counts & total scores for NanoFolder

Total Base Pair Counts
Total TP 1064
Total TN 413459
Total FP 2599
Total FP CONTRA 475
Total FP INCONS 2000
Total FP COMP 124
Total FN 1707
Total Scores
MCC 0.335
Average MCC ± 95% Confidence Intervals 0.389 ± 0.054
Sensitivity 0.384
Positive Predictive Value 0.301
Nr of predictions 117

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2. Individual counts for NanoFolder [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
CRW_01535 0.20 0.24 0.17 9 7088 43 7 36 0 28
CRW_01539 0.10 0.11 0.10 4 7099 40 1 36 3 33
CRW_01563 0.11 0.14 0.11 5 6856 43 5 37 1 32
CRW_01583 0.36 0.41 0.33 15 7095 31 8 22 1 22
CRW_01590 -0.01 0.00 0.00 0 7093 50 7 40 3 37
CRW_01593 0.32 0.38 0.29 14 7091 36 5 30 1 23
CRW_01595 -0.01 0.00 0.00 0 8459 57 10 46 1 37
CRW_01603 0.41 0.46 0.38 17 7095 29 6 22 1 20
CRW_01617 0.20 0.24 0.17 9 7087 45 7 37 1 28
CRW_01625 0.43 0.49 0.38 18 7093 31 7 22 2 19
PDB_00011 - 0.96 0.92 1.00 11 265 0 0 0 0 1
PDB_00017 - 0.66 0.67 0.67 10 688 5 1 4 0 5
PDB_00036 - -0.01 0.00 0.00 0 3457 29 1 28 0 27
PDB_00070 0.25 0.29 0.23 7 2819 24 4 20 0 17
PDB_00113 - 0.94 0.89 1.00 8 223 0 0 0 0 1
PDB_00119 - 0.95 0.90 1.00 9 244 0 0 0 0 1
PDB_00136 - 0.70 0.73 0.68 19 2387 9 1 8 0 7
PDB_00137 - 0.87 0.77 1.00 10 396 0 0 0 0 3
PDB_00160 - 0.89 0.89 0.89 8 267 2 0 1 1 1
PDB_00178 - -0.02 0.00 0.00 0 517 11 1 10 0 11
PDB_00221 - 0.94 0.89 1.00 8 245 0 0 0 0 1
PDB_00312 - 1.00 1.00 1.00 9 426 1 0 0 1 0
PDB_00326 - 0.58 0.64 0.53 18 3707 16 4 12 0 10
PDB_00341 - 0.47 0.44 0.54 7 690 6 0 6 0 9
PDB_00349 - 0.10 0.14 0.10 2 926 18 0 18 0 12
PDB_00417 - 0.00 0.00 0.00 0 14138 58 2 56 0 56
PDB_00430 0.38 0.44 0.34 11 3208 21 6 15 0 14
PDB_00527 - -0.02 0.00 0.00 0 292 8 2 6 0 5
PDB_00565 - 0.24 0.24 0.24 12 8079 37 1 36 0 38
PDB_00570 - -0.01 0.00 0.00 0 2257 21 0 21 0 22
PDB_00571 -0.01 0.00 0.00 0 3286 36 6 29 1 25
PDB_00573 - -0.01 0.00 0.00 0 6622 48 5 43 0 34
PDB_00584 - 0.47 0.53 0.43 9 1464 12 1 11 0 8
PDB_00698 - 0.97 0.95 1.00 18 843 0 0 0 0 1
PDB_00725 - 0.82 0.80 0.86 12 581 2 0 2 0 3
PDB_00734 - 0.83 0.70 1.00 7 224 0 0 0 0 3
PDB_00823 - -0.04 0.00 0.00 0 183 7 0 7 0 9
PDB_00828 0.30 0.33 0.28 9 2453 23 0 23 0 18
PDB_00851 - 0.16 0.17 0.17 7 4711 35 3 32 0 34
PDB_00890 - -0.02 0.00 0.00 0 690 13 2 11 0 14
PDB_00923 - 1.00 1.00 1.00 6 184 2 0 0 2 0
PDB_00994 - 0.40 0.50 0.33 21 10521 44 13 30 1 21
PDB_01009 0.27 0.33 0.23 7 2455 25 3 20 2 14
PDB_01051 -0.02 0.00 0.00 0 885 18 1 17 0 13
PDB_01074 - -0.01 0.00 0.00 0 2747 28 3 25 0 25
PDB_01130 - -0.01 0.00 0.00 0 1742 28 5 23 0 20
PDB_01199 - -0.01 0.00 0.00 0 2116 29 2 27 0 24
PDB_01236 0.31 0.33 0.30 18 11568 43 3 39 1 36
PDB_01275 - 0.04 0.04 0.04 3 18258 75 6 69 0 68
RFA_00416 0.94 1.00 0.88 15 1468 6 2 0 4 0
RFA_00434 0.84 1.00 0.71 15 1410 8 6 0 2 0
RFA_00436 0.86 1.00 0.75 15 1411 7 5 0 2 0
RFA_00442 0.94 1.00 0.88 15 1414 4 2 0 2 0
RFA_00446 0.85 0.93 0.78 14 1467 7 3 1 3 1
RFA_00604 0.42 0.54 0.33 19 13472 53 14 25 14 16
RFA_00664 -0.01 0.00 0.00 0 978 13 0 12 1 14
RFA_00674 0.12 0.14 0.13 2 1112 15 0 14 1 12
RFA_00675 0.28 0.29 0.29 4 976 10 1 9 0 10
RFA_00677 0.62 0.64 0.60 9 975 10 0 6 4 5
RFA_00684 0.74 0.71 0.77 10 977 5 2 1 2 4
RFA_00703 0.26 0.36 0.19 5 4252 32 5 16 11 9
RFA_00716 -0.02 0.00 0.00 0 929 17 0 17 0 14
RFA_00717 0.64 0.64 0.64 9 889 5 2 3 0 5
RFA_00730 0.38 0.42 0.36 5 889 10 3 6 1 7
RFA_00763 0.38 0.42 0.36 5 889 10 3 6 1 7
RFA_00765 0.39 0.42 0.38 5 890 9 2 6 1 7
RFA_00770 -0.01 0.00 0.00 0 1996 24 0 20 4 18
RFA_00773 0.93 1.00 0.86 18 1932 9 3 0 6 0
RFA_00808 0.83 1.00 0.70 16 1993 7 7 0 0 0
SPR_00030 - 0.47 0.52 0.42 11 2459 16 2 13 1 10
SPR_00066 0.47 0.57 0.39 12 2819 20 4 15 1 9
SPR_00228 0.44 0.60 0.32 12 3791 26 15 10 1 8
SPR_00314 0.47 0.57 0.39 12 2895 21 9 10 2 9
SPR_00331 0.48 0.60 0.39 12 2744 19 6 13 0 8
SPR_00333 0.25 0.33 0.21 7 2741 27 10 17 0 14
SPR_00353 0.64 0.81 0.52 17 2817 16 8 8 0 4
SPR_00398 - -0.01 0.00 0.00 0 2185 28 10 16 2 13
SPR_00452 0.27 0.33 0.23 7 2820 24 7 16 1 14
SPR_00456 0.49 0.57 0.43 12 2822 19 5 11 3 9
SPR_00462 0.65 0.74 0.58 14 2391 11 4 6 1 5
SPR_00476 0.47 0.52 0.42 11 2749 17 4 11 2 10
SPR_00496 0.47 0.57 0.40 12 2745 22 7 11 4 9
SPR_00497 0.27 0.33 0.23 7 2745 23 5 18 0 14
SPR_00502 0.27 0.33 0.23 7 2896 26 2 21 3 14
SPR_00509 0.23 0.30 0.19 6 2895 25 8 17 0 14
SPR_00512 0.61 0.75 0.50 15 2896 15 6 9 0 5
SPR_00542 0.26 0.33 0.22 7 3538 28 4 21 3 14
SPR_00557 0.42 0.52 0.34 11 2818 22 8 13 1 10
SPR_00832 - 0.53 0.67 0.43 10 1630 13 5 8 0 5
SPR_00868 0.49 0.57 0.43 12 2898 19 5 11 3 9
SPR_00932 0.43 0.52 0.35 11 2819 21 7 13 1 10
SPR_00957 0.63 0.76 0.53 16 2973 15 7 7 1 5
SPR_01040 0.15 0.19 0.13 4 2744 27 4 23 0 17
SPR_01143 0.41 0.48 0.36 10 2528 18 8 10 0 11
SRP_00005 - -0.01 0.00 0.00 0 3966 39 6 33 0 24
SRP_00038 0.23 0.27 0.20 9 5005 36 1 35 0 24
SRP_00046 0.33 0.38 0.29 12 4712 30 3 26 1 20
SRP_00138 - 0.44 0.52 0.39 14 3969 24 8 14 2 13
SRP_00170 0.29 0.36 0.25 9 4059 28 4 23 1 16
SRP_00193 - 0.16 0.19 0.15 7 6855 43 4 37 2 29
SRP_00194 0.27 0.33 0.23 7 3209 27 5 19 3 14
SRP_00200 0.41 0.47 0.35 17 6855 31 4 27 0 19
SRP_00216 0.44 0.48 0.41 13 3371 19 4 15 0 14
SRP_00229 0.42 0.52 0.35 14 5213 26 8 18 0 13
SRP_00233 0.25 0.30 0.22 10 5106 35 4 31 0 23
SRP_00243 0.47 0.55 0.40 17 5109 25 7 18 0 14
SRP_00244 0.38 0.44 0.34 17 6853 33 2 31 0 22
SRP_00266 0.55 0.66 0.46 19 4615 22 8 14 0 10
SRP_00269 0.46 0.52 0.43 17 5010 24 6 17 1 16
SRP_00273 0.36 0.40 0.33 16 6737 34 6 27 1 24
SRP_00285 0.38 0.43 0.35 13 3704 24 6 18 0 17
SRP_00303 0.36 0.41 0.33 13 6630 27 3 24 0 19
SRP_00333 0.20 0.24 0.19 8 5108 35 3 32 0 26
SRP_00338 0.52 0.56 0.49 20 5419 22 4 17 1 16
SRP_00342 0.20 0.24 0.17 6 3970 29 7 22 0 19
SRP_00358 0.32 0.37 0.28 13 6282 33 4 29 0 22
SRP_00383 -0.01 0.00 0.00 0 3205 35 9 26 0 24

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.