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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of MaxExpect - scored higher in this pairwise comparison

  4. Performance of PknotsRG - scored lower in this pairwise comparison

  5. Compile and download dataset for MaxExpect & PknotsRG [.zip] - may take several seconds...


Overview

Metric MaxExpect PknotsRG
MCC 0.670 > 0.616
Average MCC ± 95% Confidence Intervals 0.742 ± 0.059 < 0.758 ± 0.054
Sensitivity 0.685 > 0.646
Positive Predictive Value 0.656 > 0.589
Total TP 2341 > 2207
Total TN 2127742 > 2127563
Total FP 1642 < 1939
Total FP CONTRA 371 < 419
Total FP INCONS 854 < 1119
Total FP COMP 417 > 401
Total FN 1076 < 1210
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of MaxExpect and PknotsRG. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for MaxExpect and PknotsRG).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for MaxExpect and PknotsRG).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for MaxExpect and PknotsRG. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for MaxExpect and PknotsRG).

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Performance of MaxExpect - scored higher in this pairwise comparison

1. Total counts & total scores for MaxExpect

Total Base Pair Counts
Total TP 2341
Total TN 2127742
Total FP 1642
Total FP CONTRA 371
Total FP INCONS 854
Total FP COMP 417
Total FN 1076
Total Scores
MCC 0.670
Average MCC ± 95% Confidence Intervals 0.742 ± 0.059
Sensitivity 0.685
Positive Predictive Value 0.656
Nr of predictions 117

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2. Individual counts for MaxExpect [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KE6_A 0.94 0.94 0.94 17 449 2 0 1 1 1
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.91 0.87 0.95 20 2003 9 1 0 8 3
2KU0_A - -0.04 0.00 0.00 0 283 9 1 8 0 12
2KUR_A 0.95 0.95 0.95 18 448 1 0 1 0 1
2KUU_A 0.94 0.94 0.94 17 429 2 0 1 1 1
2KUV_A 0.94 0.95 0.95 18 420 1 0 1 0 1
2KUW_A 0.94 0.94 0.94 17 452 2 0 1 1 1
2KX8_A 1.00 1.00 1.00 16 355 0 0 0 0 0
2KXM_A - 0.93 0.88 1.00 7 142 0 0 0 0 1
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_B 1.00 1.00 1.00 24 767 0 0 0 0 0
2L1F_A 1.00 1.00 1.00 23 740 0 0 0 0 0
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 1.00 1.00 1.00 14 225 0 0 0 0 0
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A -0.03 0.00 0.00 0 513 15 3 12 0 18
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 0.84 0.83 0.86 24 2412 11 0 4 7 5
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 1.00 1.00 1.00 11 196 1 0 0 1 0
2RRC_A - 0.83 0.71 1.00 5 70 0 0 0 0 2
2WRQ_Y 0.94 0.89 1.00 8 1144 14 0 0 14 1
2WW9_F - 0.64 0.75 0.60 6 102 4 1 3 0 2
2WW9_E - -0.03 0.00 0.00 0 168 5 0 4 1 5
2WW9_D - 0.08 0.10 0.08 1 729 15 2 9 4 9
2WWQ_V 0.97 0.95 1.00 18 1186 3 0 0 3 1
2XKV_B 0.64 0.73 0.57 8 1821 26 0 6 20 3
2XQD_Y 0.98 0.95 1.00 20 1109 1 0 0 1 1
2XXA_G 0.24 0.26 0.25 9 2009 28 1 26 1 26
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_X - 0.53 0.57 0.50 4 536 6 1 3 2 3
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3ADB_C - 0.86 0.85 0.88 28 1787 4 0 4 0 5
3AKZ_H 1.00 1.00 1.00 20 1107 2 0 0 2 0
3AM1_B - 0.86 0.86 0.86 25 1437 4 0 4 0 4
3AMU_B 0.73 0.79 0.68 15 1135 10 0 7 3 4
3GX2_A 0.93 0.89 0.96 25 1423 2 1 0 1 3
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3IVN_B 0.91 0.83 1.00 19 884 0 0 0 0 4
3IWN_A 0.80 0.79 0.81 22 1445 6 1 4 1 6
3IYQ_A 0.24 0.33 0.17 17 22342 96 41 40 15 34
3IZ4_A 0.61 0.61 0.62 58 25442 42 14 22 6 37
3IZF_C 0.89 0.91 0.86 32 2603 8 0 5 3 3
3J0L_7 - -0.02 0.00 0.00 0 507 12 3 9 0 10
3J0L_h - 0.93 0.88 1.00 28 2112 2 0 0 2 4
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_a - 0.39 0.36 0.44 4 402 6 4 1 1 7
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_g - -0.01 0.00 0.00 0 174 4 0 2 2 2
3J0L_2 - 0.30 0.31 0.31 8 2224 25 2 16 7 18
3J16_L 0.34 0.33 0.37 7 1140 12 0 12 0 14
3J20_0 0.53 0.57 0.50 12 1195 13 2 10 1 9
3J20_1 0.75 0.75 0.75 15 1092 6 0 5 1 5
3J2C_O - 0.74 0.71 0.78 29 3950 13 0 8 5 12
3J2C_M - 0.48 0.54 0.43 52 39804 88 29 40 19 44
3J2L_3 0.80 0.82 0.78 28 2984 12 1 7 4 6
3JYV_7 0.48 0.45 0.53 9 1094 9 3 5 1 11
3JYX_3 0.77 0.80 0.75 12 2362 24 0 4 20 3
3JYX_4 0.55 0.83 0.37 10 4729 34 14 3 17 2
3KIY_A - 0.67 0.68 0.66 531 1485073 336 81 195 60 255
3KTW_C - 0.72 0.72 0.72 18 1755 14 2 5 7 7
3LA5_A 0.91 0.84 1.00 21 933 0 0 0 0 4
3NDB_M - 0.94 0.93 0.95 41 3671 7 0 2 5 3
3NKB_B - 0.69 0.74 0.67 14 714 7 0 7 0 5
3NMU_E - -0.02 0.00 0.00 0 208 10 5 3 2 3
3NPB_A 0.77 0.73 0.82 27 2245 10 0 6 4 10
3O58_3 0.40 0.50 0.33 11 4731 36 8 14 14 11
3O58_2 0.91 0.94 0.88 29 2721 11 0 4 7 2
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.92 0.90 0.94 45 4792 5 1 2 2 5
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.70 0.69 0.71 20 1505 8 3 5 0 9
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 1.00 1.00 1.00 12 142 0 0 0 0 0
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.58 0.59 0.59 13 1254 9 2 7 0 9
3UZL_B 1.00 1.00 1.00 16 1277 8 0 0 8 0
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
3W3S_B 0.97 0.94 1.00 31 1958 1 0 0 1 2
3ZEX_G - 0.98 0.96 1.00 43 6464 17 0 0 17 2
3ZEX_C 0.54 0.45 0.65 13 5354 17 2 5 10 16
3ZEX_H - 0.26 0.37 0.19 7 3589 29 18 11 0 12
3ZEX_F - -0.01 0.00 0.00 0 908 11 2 4 5 4
3ZEX_D 0.82 0.77 0.87 27 2765 11 0 4 7 8
3ZEX_E - -0.01 0.00 0.00 0 8252 54 21 31 2 34
3ZEX_B - 0.37 0.39 0.35 134 420964 302 66 188 48 212
4A1C_2 0.19 0.25 0.16 5 4484 41 10 17 14 15
4A1C_3 0.83 0.81 0.86 30 2728 7 0 5 2 7
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.60 0.59 0.63 17 1410 11 4 6 1 12
4ATO_G - 0.45 0.43 0.50 3 214 3 3 0 0 4
4ENB_A 0.85 0.73 1.00 11 461 0 0 0 0 4
4ENC_A 0.36 0.33 0.42 5 484 8 0 7 1 10
4FNJ_A - 0.91 0.83 1.00 10 240 0 0 0 0 2
4FRG_B 0.29 0.25 0.35 6 1185 11 4 7 0 18
4FRN_A 0.51 0.46 0.57 13 1825 10 5 5 0 15
4HXH_A - 1.00 1.00 1.00 6 89 0 0 0 0 0

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Performance of PknotsRG - scored lower in this pairwise comparison

1. Total counts & total scores for PknotsRG

Total Base Pair Counts
Total TP 2207
Total TN 2127563
Total FP 1939
Total FP CONTRA 419
Total FP INCONS 1119
Total FP COMP 401
Total FN 1210
Total Scores
MCC 0.616
Average MCC ± 95% Confidence Intervals 0.758 ± 0.054
Sensitivity 0.646
Positive Predictive Value 0.589
Nr of predictions 117

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2. Individual counts for PknotsRG [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2KD8_A - 1.00 1.00 1.00 9 99 0 0 0 0 0
2KE6_A 0.88 0.89 0.89 16 449 3 0 2 1 2
2KPV_A - 0.96 0.92 1.00 12 201 0 0 0 0 1
2KRL_A - 0.85 0.87 0.83 20 2000 12 4 0 8 3
2KU0_A - 0.96 0.92 1.00 11 281 0 0 0 0 1
2KUR_A 0.89 0.89 0.89 17 448 2 0 2 0 2
2KUU_A 0.88 0.89 0.89 16 429 3 0 2 1 2
2KUV_A 0.89 0.89 0.89 17 420 2 0 2 0 2
2KUW_A 0.94 0.94 0.94 17 452 2 0 1 1 1
2KX8_A 1.00 1.00 1.00 16 355 0 0 0 0 0
2KXM_A - 0.93 0.88 1.00 7 142 0 0 0 0 1
2KZL_A - 1.00 1.00 1.00 13 507 1 0 0 1 0
2L1F_B 1.00 1.00 1.00 24 767 0 0 0 0 0
2L1F_A 1.00 1.00 1.00 23 740 0 0 0 0 0
2L2K_A - 1.00 1.00 1.00 17 332 0 0 0 0 0
2L3C_B - 1.00 1.00 1.00 14 225 0 0 0 0 0
2L3E_A - 1.00 1.00 1.00 12 228 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 30 955 0 0 0 0 0
2L5Z_A - 1.00 1.00 1.00 9 102 0 0 0 0 0
2L94_A 1.00 1.00 1.00 18 339 1 0 0 1 0
2LA5_A - 0.77 0.71 0.83 5 269 3 0 1 2 2
2LBS_A - 1.00 1.00 1.00 14 193 0 0 0 0 0
2LC8_A 0.66 0.61 0.73 11 513 4 2 2 0 7
2LDL_A - 1.00 1.00 1.00 9 131 1 0 0 1 0
2LDT_A - 1.00 1.00 1.00 11 151 0 0 0 0 0
2LHP_A - 1.00 1.00 1.00 15 246 0 0 0 0 0
2LI4_A - 1.00 1.00 1.00 14 175 0 0 0 0 0
2LJJ_A - 1.00 1.00 1.00 7 123 3 0 0 3 0
2LK3_A - 1.00 1.00 1.00 9 91 0 0 0 0 0
2LKR_A - 0.81 0.86 0.76 25 2407 10 6 2 2 4
2LQZ_A - 1.00 1.00 1.00 8 124 2 0 0 2 0
2LWK_A - 0.95 0.91 1.00 10 197 1 0 0 1 1
2RRC_A - 0.83 0.71 1.00 5 70 0 0 0 0 2
2WRQ_Y 0.94 0.89 1.00 8 1144 14 0 0 14 1
2WW9_F - 0.64 0.75 0.60 6 102 4 1 3 0 2
2WW9_E - 0.89 0.80 1.00 4 168 0 0 0 0 1
2WW9_D - 0.08 0.10 0.08 1 729 15 2 9 4 9
2WWQ_V 1.00 1.00 1.00 19 1185 5 0 0 5 0
2XKV_B 0.64 0.73 0.57 8 1821 24 0 6 18 3
2XQD_Y 0.98 0.95 1.00 20 1109 1 0 0 1 1
2XXA_G 0.35 0.34 0.38 12 2013 21 1 19 1 23
2Y8W_B - 1.00 1.00 1.00 6 82 1 0 0 1 0
2Y9C_V - 0.79 0.83 0.75 15 886 8 0 5 3 3
2YIE_X - 0.43 0.57 0.33 4 532 10 2 6 2 3
2YIE_Z - -0.02 0.00 0.00 0 587 15 6 9 0 8
3A2K_C 0.47 0.50 0.46 11 1084 13 3 10 0 11
3ADB_C - 0.35 0.36 0.36 12 1786 21 1 20 0 21
3AKZ_H 0.46 0.50 0.43 10 1104 15 6 7 2 10
3AM1_B - 0.74 0.76 0.73 22 1436 8 3 5 0 7
3AMU_B 0.81 0.79 0.83 15 1139 6 0 3 3 4
3GX2_A 0.80 0.79 0.81 22 1422 6 2 3 1 6
3IAB_R - 1.00 1.00 1.00 12 373 0 0 0 0 0
3IVN_B 0.91 0.83 1.00 19 884 0 0 0 0 4
3IWN_A 0.21 0.21 0.24 6 1447 19 1 18 0 22
3IYQ_A 0.24 0.33 0.17 17 22340 94 45 38 11 34
3IZ4_A 0.56 0.60 0.52 57 25426 57 25 28 4 38
3IZF_C 0.75 0.77 0.73 27 2603 15 0 10 5 8
3J0L_7 - 0.38 0.50 0.31 5 503 12 3 8 1 5
3J0L_h - 0.87 0.81 0.93 26 2112 4 1 1 2 6
3J0L_1 - 0.83 0.77 0.91 10 473 4 0 1 3 3
3J0L_a - 0.18 0.18 0.22 2 402 8 3 4 1 9
3J0L_8 - 1.00 1.00 1.00 7 69 0 0 0 0 0
3J0L_g - -0.01 0.00 0.00 0 174 4 0 2 2 2
3J0L_2 - 0.29 0.31 0.29 8 2222 27 2 18 7 18
3J16_L 0.90 0.81 1.00 17 1142 0 0 0 0 4
3J20_0 0.51 0.57 0.48 12 1194 14 3 10 1 9
3J20_1 1.00 1.00 1.00 20 1092 1 0 0 1 0
3J2C_O - 0.71 0.71 0.73 29 3947 17 2 9 6 12
3J2C_M - 0.64 0.71 0.59 68 39809 75 20 28 27 28
3J2L_3 0.80 0.82 0.78 28 2984 12 1 7 4 6
3JYV_7 -0.02 0.00 0.00 0 1089 22 4 18 0 20
3JYX_3 0.77 0.80 0.75 12 2362 27 0 4 23 3
3JYX_4 0.61 0.83 0.45 10 4734 33 10 2 21 2
3KIY_A - 0.47 0.50 0.45 393 1485010 525 91 386 48 393
3KTW_C - 1.00 1.00 1.00 25 1755 7 0 0 7 0
3LA5_A 0.91 0.84 1.00 21 933 0 0 0 0 4
3NDB_M - 0.93 0.93 0.93 41 3670 9 0 3 6 3
3NKB_B - 0.69 0.74 0.67 14 714 7 0 7 0 5
3NMU_E - 1.00 1.00 1.00 3 213 5 0 0 5 0
3NPB_A 0.93 0.86 1.00 32 2246 4 0 0 4 5
3O58_3 0.41 0.50 0.34 11 4732 35 6 15 14 11
3O58_2 0.72 0.74 0.70 23 2721 19 0 10 9 8
3OVB_D - 1.00 1.00 1.00 11 215 1 0 0 1 0
3OVS_D - 1.00 1.00 1.00 12 204 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 11 301 0 0 0 0 0
3PDR_A 0.90 0.90 0.90 45 4790 7 2 3 2 5
3R4F_A - 1.00 1.00 1.00 22 884 1 0 0 1 0
3R9X_C - 1.00 1.00 1.00 8 221 2 0 0 2 0
3RKF_A 0.91 0.83 1.00 20 846 0 0 0 0 4
3SD1_A 0.73 0.72 0.75 21 1505 7 4 3 0 8
3SIU_F - 1.00 1.00 1.00 8 137 0 0 0 0 0
3SN2_B 0.95 0.92 1.00 11 143 0 0 0 0 1
3TRZ_Z - 1.00 1.00 1.00 5 87 1 0 0 1 0
3TS0_U - 1.00 1.00 1.00 6 112 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 103 0 0 0 0 0
3U4M_B - 0.49 0.55 0.46 12 1250 14 3 11 0 10
3UZL_B 0.49 0.50 0.50 8 1277 15 0 8 7 8
3VJR_D - 1.00 1.00 1.00 12 239 0 0 0 0 0
3W3S_B 0.64 0.64 0.66 21 1957 12 4 7 1 12
3ZEX_G - 0.70 0.71 0.70 32 6461 23 6 8 9 13
3ZEX_C 0.30 0.34 0.26 10 5336 44 5 23 16 19
3ZEX_H - 0.25 0.37 0.18 7 3586 32 20 12 0 12
3ZEX_F - -0.01 0.00 0.00 0 908 11 2 4 5 4
3ZEX_D 0.88 0.83 0.94 29 2765 6 0 2 4 6
3ZEX_E - 0.00 0.00 0.00 0 8254 55 17 33 5 34
3ZEX_B - 0.36 0.40 0.33 140 420927 323 78 207 38 206
4A1C_2 0.18 0.25 0.14 5 4480 43 11 20 12 15
4A1C_3 0.85 0.84 0.86 31 2727 8 0 5 3 6
4A4U_A - 1.00 1.00 1.00 9 96 0 0 0 0 0
4AOB_A 0.60 0.59 0.63 17 1410 11 4 6 1 12
4ATO_G - 0.88 1.00 0.78 7 211 2 2 0 0 0
4ENB_A 1.00 1.00 1.00 15 457 0 0 0 0 0
4ENC_A 0.97 1.00 0.94 15 480 1 1 0 0 0
4FNJ_A - 0.91 0.83 1.00 10 240 0 0 0 0 2
4FRG_B 0.36 0.38 0.38 9 1178 15 2 13 0 15
4FRN_A 0.59 0.57 0.62 16 1822 10 2 8 0 12
4HXH_A - 1.00 1.00 1.00 6 89 0 0 0 0 0

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.