CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of HotKnots - scored higher in this pairwise comparison

  4. Performance of MaxExpect - scored lower in this pairwise comparison

  5. Compile and download dataset for HotKnots & MaxExpect [.zip] - may take several seconds...


Overview

Metric HotKnots MaxExpect
MCC 0.617 > 0.610
Average MCC ± 95% Confidence Intervals 0.689 ± 0.052 > 0.666 ± 0.056
Sensitivity 0.556 > 0.539
Positive Predictive Value 0.690 < 0.695
Total TP 1821 > 1766
Total TN 589439 < 589537
Total FP 936 > 914
Total FP CONTRA 120 > 94
Total FP INCONS 699 > 682
Total FP COMP 117 < 138
Total FN 1457 < 1512
P-value 1.50402730567e-05

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Performance plots


  1. Comparison of performance of HotKnots and MaxExpect. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for HotKnots and MaxExpect).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for HotKnots and MaxExpect).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for HotKnots and MaxExpect. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for HotKnots and MaxExpect).

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Performance of HotKnots - scored higher in this pairwise comparison

1. Total counts & total scores for HotKnots

Total Base Pair Counts
Total TP 1821
Total TN 589439
Total FP 936
Total FP CONTRA 120
Total FP INCONS 699
Total FP COMP 117
Total FN 1457
Total Scores
MCC 0.617
Average MCC ± 95% Confidence Intervals 0.689 ± 0.052
Sensitivity 0.556
Positive Predictive Value 0.690
Nr of predictions 115

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2. Individual counts for HotKnots [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2KD8_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
2KE6_A 0.97 0.95 1.00 18 1110 1 0 0 1 1
2KPV_A - 0.96 0.92 1.00 12 549 0 0 0 0 1
2KRL_A - 0.76 0.60 0.96 24 5126 5 0 1 4 16
2KU0_A - 0.83 0.69 1.00 11 692 0 0 0 0 5
2KUR_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KUU_A 0.92 0.86 1.00 18 1110 1 0 0 1 3
2KUV_A 0.93 0.86 1.00 19 1109 0 0 0 0 3
2KUW_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KX8_A 0.94 0.89 1.00 16 845 0 0 0 0 2
2KXM_A - 0.89 0.80 1.00 8 343 0 0 0 0 2
2KZL_A - 0.86 0.74 1.00 14 1471 0 0 0 0 5
2L1F_A 0.98 0.96 1.00 23 2057 0 0 0 0 1
2L1F_B 0.98 0.96 1.00 24 2121 0 0 0 0 1
2L2K_A - 0.94 0.89 1.00 17 844 0 0 0 0 2
2L3C_B - 0.91 0.82 1.00 14 547 0 0 0 0 3
2L3E_A - 0.96 0.92 1.00 12 583 1 0 0 1 1
2L3J_B 0.94 0.88 1.00 30 2455 0 0 0 0 4
2L5Z_A - 0.86 0.75 1.00 9 316 0 0 0 0 3
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - 0.39 0.26 0.63 5 622 3 1 2 0 14
2LBS_A - 1.00 1.00 1.00 14 482 0 0 0 0 0
2LC8_A 0.61 0.55 0.69 11 1524 5 0 5 0 9
2LDL_A - 0.90 0.82 1.00 9 342 1 0 0 1 2
2LDT_A - 0.85 0.73 1.00 11 454 0 0 0 0 4
2LHP_A - 0.97 0.94 1.00 15 651 0 0 0 0 1
2LI4_A - 0.93 0.88 1.00 14 482 0 0 0 0 2
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 0.95 0.90 1.00 9 267 0 0 0 0 1
2LKR_A - 0.93 0.87 1.00 34 6071 3 0 0 3 5
2LQZ_A - 0.85 0.82 0.90 9 341 1 1 0 0 2
2LWK_A - 0.83 0.77 0.91 10 485 1 0 1 0 3
2RRC_A - 0.83 0.70 1.00 7 224 0 0 0 0 3
2WRQ_Y 0.57 0.59 0.56 10 2832 13 5 3 5 7
2WW9_D - 0.14 0.12 0.19 3 1937 13 0 13 0 23
2WW9_E - 0.53 0.29 1.00 4 557 0 0 0 0 10
2WW9_F - 0.59 0.60 0.60 6 290 4 0 4 0 4
2WWQ_V 0.76 0.68 0.86 19 2904 5 0 3 2 9
2XKV_B 0.51 0.50 0.53 10 4541 23 0 9 14 10
2XQD_Y 0.88 0.78 1.00 21 2829 0 0 0 0 6
2XXA_G 0.34 0.31 0.39 13 5118 20 1 19 0 29
2Y8W_B - 1.00 1.00 1.00 6 184 1 0 0 1 0
2Y9C_V - 0.65 0.63 0.68 15 2256 8 1 6 1 9
2YIE_X - 0.36 0.33 0.40 4 1368 6 2 4 0 8
2YIE_Z - -0.01 0.00 0.00 0 1525 15 3 12 0 12
3A2K_C 0.42 0.39 0.46 11 2902 13 2 11 0 17
3ADB_C - 0.79 0.74 0.85 28 4153 5 0 5 0 10
3AKZ_H 0.43 0.39 0.48 11 2678 12 2 10 0 17
3AM1_B - 0.78 0.71 0.86 25 3211 4 0 4 0 10
3AMU_B 0.70 0.59 0.84 16 2984 5 0 3 2 11
3GX2_A 0.68 0.55 0.85 22 4345 5 0 4 1 18
3IAB_R - 0.92 0.86 1.00 12 1023 0 0 0 0 2
3IVN_B 0.78 0.61 1.00 19 2327 0 0 0 0 12
3IWN_A 0.63 0.58 0.70 19 4251 9 0 8 1 14
3IYQ_A 0.31 0.34 0.29 32 60617 81 22 55 4 62
3IZ4_A 0.51 0.47 0.55 62 70763 52 10 41 1 70
3IZF_C 0.70 0.61 0.80 33 6862 8 1 7 0 21
3J0L_g - -0.01 0.00 0.00 0 460 5 1 4 0 4
3J0L_7 - 0.28 0.29 0.29 5 1208 12 0 12 0 12
3J0L_h - 0.75 0.60 0.93 26 6077 4 0 2 2 17
3J0L_1 - 0.76 0.63 0.92 12 1212 2 0 1 1 7
3J0L_a - 0.51 0.50 0.53 8 1113 7 3 4 0 8
3J0L_8 - 0.93 0.88 1.00 7 183 0 0 0 0 1
3J0L_2 - 0.28 0.27 0.29 9 6185 23 3 19 1 24
3J16_L 0.26 0.23 0.30 7 2752 16 1 15 0 23
3J20_0 0.45 0.40 0.52 12 2827 12 0 11 1 18
3J20_1 0.73 0.70 0.76 16 2905 7 0 5 2 7
3J2C_O - 0.60 0.49 0.74 31 10254 12 0 11 1 32
3J2C_M - 0.61 0.49 0.76 102 106356 38 5 28 5 105
3J2L_3 0.62 0.53 0.74 28 7837 12 0 10 2 25
3JYV_7 -0.01 0.00 0.00 0 2828 22 1 21 0 32
3JYX_4 0.32 0.30 0.33 10 12216 31 5 15 11 23
3JYX_3 0.62 0.63 0.61 17 6300 22 1 10 11 10
3KTW_C - 0.73 0.63 0.84 27 4528 6 2 3 1 16
3LA5_A 0.78 0.62 1.00 21 2464 0 0 0 0 13
3NDB_M - 0.84 0.74 0.96 45 9133 3 0 2 1 16
3NKB_B - 0.59 0.54 0.67 14 1995 7 0 7 0 12
3NMU_E - 0.54 0.50 0.60 3 556 5 0 2 3 3
3NPB_A 0.78 0.65 0.94 30 6989 5 0 2 3 16
3O58_2 0.71 0.71 0.71 27 7222 12 4 7 1 11
3O58_3 0.23 0.26 0.21 9 12360 34 10 24 0 26
3OVB_D - 1.00 1.00 1.00 11 584 1 0 0 1 0
3OVS_D - 0.96 0.92 1.00 12 549 0 0 0 0 1
3P22_G - 0.96 0.92 1.00 11 730 0 0 0 0 1
3PDR_A 0.67 0.56 0.82 40 12831 11 0 9 2 32
3R4F_A - 0.96 0.92 1.00 23 2122 0 0 0 0 2
3R9X_C - 0.84 0.80 0.89 8 586 2 0 1 1 2
3RKF_A 0.76 0.59 1.00 20 2191 0 0 0 0 14
3SD1_A 0.64 0.52 0.79 22 3888 6 1 5 0 20
3SIU_F - 0.85 0.73 1.00 8 370 0 0 0 0 3
3SN2_B 0.96 0.92 1.00 11 395 0 0 0 0 1
3TRZ_Z - 0.91 0.83 1.00 5 205 1 0 0 1 1
3TS0_U - 1.00 1.00 1.00 6 247 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 271 0 0 0 0 0
3U4M_B - 0.38 0.32 0.46 12 3134 14 0 14 0 25
3UZL_B 0.48 0.38 0.61 14 3547 9 0 9 0 23
3VJR_D - 0.96 0.92 1.00 12 618 0 0 0 0 1
3W3S_B 0.55 0.50 0.61 20 4720 14 1 12 1 20
3ZEX_F - 0.00 0.00 0.00 0 2617 11 2 9 0 12
3ZEX_C 0.00 0.00 0.00 0 14150 46 6 40 0 52
3ZEX_G - 0.66 0.57 0.76 42 16416 18 0 13 5 32
3ZEX_D 0.78 0.67 0.92 33 6985 3 0 3 0 16
3ZEX_E - 0.00 0.00 0.00 0 21893 54 4 48 2 77
3ZEX_H - 0.18 0.18 0.19 7 9008 30 5 25 0 31
4A1C_2 0.14 0.15 0.14 5 11745 42 6 25 11 28
4A1C_3 0.70 0.59 0.82 32 7101 7 1 6 0 22
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4AOB_A 0.50 0.40 0.63 17 4344 11 2 8 1 25
4ATO_G - 0.61 0.70 0.54 7 515 6 2 4 0 3
4ENB_A 0.89 0.79 1.00 15 1260 0 0 0 0 4
4ENC_A 0.86 0.79 0.94 15 1310 1 1 0 0 4
4FNJ_A - 0.79 0.63 1.00 10 585 0 0 0 0 6
4FRG_B 0.32 0.28 0.38 9 3462 15 0 15 0 23
4FRN_A 0.52 0.44 0.62 16 5125 10 2 8 0 20
4HXH_A - 1.00 1.00 1.00 6 319 0 0 0 0 0

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Performance of MaxExpect - scored lower in this pairwise comparison

1. Total counts & total scores for MaxExpect

Total Base Pair Counts
Total TP 1766
Total TN 589537
Total FP 914
Total FP CONTRA 94
Total FP INCONS 682
Total FP COMP 138
Total FN 1512
Total Scores
MCC 0.610
Average MCC ± 95% Confidence Intervals 0.666 ± 0.056
Sensitivity 0.539
Positive Predictive Value 0.695
Nr of predictions 115

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2. Individual counts for MaxExpect [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2KD8_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
2KE6_A 0.92 0.89 0.94 17 1110 2 0 1 1 2
2KPV_A - 0.96 0.92 1.00 12 549 0 0 0 0 1
2KRL_A - 0.76 0.60 0.96 24 5126 5 0 1 4 16
2KU0_A - -0.02 0.00 0.00 0 694 9 0 9 0 16
2KUR_A 0.90 0.86 0.95 18 1109 1 0 1 0 3
2KUU_A 0.87 0.81 0.94 17 1110 2 0 1 1 4
2KUV_A 0.88 0.82 0.95 18 1109 1 0 1 0 4
2KUW_A 0.90 0.86 0.95 18 1109 1 0 1 0 3
2KX8_A 0.94 0.89 1.00 16 845 0 0 0 0 2
2KXM_A - 0.83 0.70 1.00 7 344 0 0 0 0 3
2KZL_A - 0.86 0.74 1.00 14 1471 0 0 0 0 5
2L1F_A 0.98 0.96 1.00 23 2057 0 0 0 0 1
2L1F_B 0.98 0.96 1.00 24 2121 0 0 0 0 1
2L2K_A - 0.94 0.89 1.00 17 844 0 0 0 0 2
2L3C_B - 0.91 0.82 1.00 14 547 0 0 0 0 3
2L3E_A - 0.96 0.92 1.00 12 583 1 0 0 1 1
2L3J_B 0.94 0.88 1.00 30 2455 0 0 0 0 4
2L5Z_A - 0.86 0.75 1.00 9 316 0 0 0 0 3
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - 0.39 0.26 0.63 5 622 3 1 2 0 14
2LBS_A - 1.00 1.00 1.00 14 482 0 0 0 0 0
2LC8_A -0.01 0.00 0.00 0 1525 15 2 13 0 20
2LDL_A - 0.90 0.82 1.00 9 342 1 0 0 1 2
2LDT_A - 0.85 0.73 1.00 11 454 0 0 0 0 4
2LHP_A - 0.97 0.94 1.00 15 651 0 0 0 0 1
2LI4_A - 0.93 0.88 1.00 14 482 0 0 0 0 2
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 0.95 0.90 1.00 9 267 0 0 0 0 1
2LKR_A - 0.81 0.74 0.88 29 6072 6 0 4 2 10
2LQZ_A - 0.85 0.82 0.90 9 341 1 1 0 0 2
2LWK_A - 0.88 0.85 0.92 11 484 1 0 1 0 2
2RRC_A - 0.70 0.50 1.00 5 226 0 0 0 0 5
2WRQ_Y 0.53 0.53 0.53 9 2833 13 5 3 5 8
2WW9_D - 0.14 0.12 0.19 3 1937 13 0 13 0 23
2WW9_E - -0.01 0.00 0.00 0 556 5 0 5 0 14
2WW9_F - 0.69 0.70 0.70 7 290 3 0 3 0 3
2WWQ_V 0.76 0.64 0.90 18 2906 3 0 2 1 10
2XKV_B 0.51 0.50 0.53 10 4541 24 0 9 15 10
2XQD_Y 0.88 0.78 1.00 21 2829 0 0 0 0 6
2XXA_G 0.25 0.24 0.27 10 5114 27 1 26 0 32
2Y8W_B - 1.00 1.00 1.00 6 184 1 0 0 1 0
2Y9C_V - 0.65 0.63 0.68 15 2256 8 1 6 1 9
2YIE_X - 0.36 0.33 0.40 4 1368 6 2 4 0 8
2YIE_Z - -0.01 0.00 0.00 0 1525 15 3 12 0 12
3A2K_C 0.42 0.39 0.46 11 2902 13 2 11 0 17
3ADB_C - 0.80 0.74 0.88 28 4154 4 0 4 0 10
3AKZ_H 0.84 0.75 0.95 21 2679 1 1 0 0 7
3AM1_B - 0.78 0.71 0.86 25 3211 4 0 4 0 10
3AMU_B 0.64 0.59 0.70 16 2980 9 0 7 2 11
3GX2_A 0.77 0.63 0.96 25 4345 2 0 1 1 15
3IAB_R - 0.92 0.86 1.00 12 1023 0 0 0 0 2
3IVN_B 0.78 0.61 1.00 19 2327 0 0 0 0 12
3IWN_A 0.74 0.67 0.81 22 4251 6 0 5 1 11
3IYQ_A 0.29 0.31 0.27 29 60617 84 19 61 4 65
3IZ4_A 0.54 0.46 0.64 61 70781 39 3 31 5 71
3IZF_C 0.71 0.61 0.83 33 6863 7 1 6 0 21
3J0L_g - -0.01 0.00 0.00 0 461 4 1 3 0 4
3J0L_7 - -0.01 0.00 0.00 0 1213 12 0 12 0 17
3J0L_h - 0.81 0.65 1.00 28 6077 2 0 0 2 15
3J0L_1 - 0.76 0.63 0.92 12 1212 2 0 1 1 7
3J0L_a - 0.39 0.31 0.50 5 1118 5 2 3 0 11
3J0L_8 - 0.93 0.88 1.00 7 183 0 0 0 0 1
3J0L_2 - 0.25 0.24 0.26 8 6185 25 2 21 2 25
3J16_L 0.29 0.23 0.37 7 2756 12 0 12 0 23
3J20_0 0.44 0.40 0.50 12 2826 13 0 12 1 18
3J20_1 0.73 0.70 0.76 16 2905 5 0 5 0 7
3J2C_O - 0.63 0.51 0.78 32 10255 10 0 9 1 31
3J2C_M - 0.38 0.31 0.47 64 106354 76 10 63 3 143
3J2L_3 0.62 0.53 0.74 28 7837 12 0 10 2 25
3JYV_7 0.41 0.31 0.56 10 2832 8 0 8 0 22
3JYX_4 0.31 0.30 0.31 10 12214 34 4 18 12 23
3JYX_3 0.64 0.63 0.65 17 6302 19 1 8 10 10
3KTW_C - 0.54 0.47 0.65 20 4529 12 2 9 1 23
3LA5_A 0.78 0.62 1.00 21 2464 0 0 0 0 13
3NDB_M - 0.78 0.69 0.89 42 9133 6 0 5 1 19
3NKB_B - 0.59 0.54 0.67 14 1995 7 0 7 0 12
3NMU_E - -0.01 0.00 0.00 0 553 10 1 7 2 6
3NPB_A 0.71 0.61 0.82 28 6987 9 0 6 3 18
3O58_2 0.75 0.76 0.74 29 7221 11 3 7 1 9
3O58_3 0.35 0.34 0.35 12 12369 35 2 20 13 23
3OVB_D - 1.00 1.00 1.00 11 584 1 0 0 1 0
3OVS_D - 0.96 0.92 1.00 12 549 0 0 0 0 1
3P22_G - 0.96 0.92 1.00 11 730 0 0 0 0 1
3PDR_A 0.76 0.63 0.94 45 12832 5 0 3 2 27
3R4F_A - 0.96 0.92 1.00 23 2122 0 0 0 0 2
3R9X_C - 0.84 0.80 0.89 8 586 2 0 1 1 2
3RKF_A 0.76 0.59 1.00 20 2191 0 0 0 0 14
3SD1_A 0.58 0.48 0.71 20 3888 8 1 7 0 22
3SIU_F - 0.85 0.73 1.00 8 370 0 0 0 0 3
3SN2_B 1.00 1.00 1.00 12 394 0 0 0 0 0
3TRZ_Z - 0.91 0.83 1.00 5 205 1 0 0 1 1
3TS0_U - 1.00 1.00 1.00 6 247 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 271 0 0 0 0 0
3U4M_B - 0.45 0.35 0.59 13 3138 9 1 8 0 24
3UZL_B 0.77 0.62 0.96 23 3546 1 0 1 0 14
3VJR_D - 0.96 0.92 1.00 12 618 0 0 0 0 1
3W3S_B 0.88 0.78 1.00 31 4722 1 0 0 1 9
3ZEX_F - 0.00 0.00 0.00 0 2617 11 2 9 0 12
3ZEX_C 0.41 0.27 0.64 14 14174 16 2 6 8 38
3ZEX_G - 0.77 0.66 0.91 49 16417 11 0 5 6 25
3ZEX_D 0.69 0.61 0.79 30 6983 8 1 7 0 19
3ZEX_E - 0.00 0.00 0.00 0 21893 54 2 50 2 77
3ZEX_H - 0.19 0.18 0.19 7 9009 29 5 24 0 31
4A1C_2 0.14 0.15 0.14 5 11746 41 4 26 11 28
4A1C_3 0.69 0.57 0.84 31 7103 6 0 6 0 23
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4AOB_A 0.50 0.40 0.63 17 4344 11 2 8 1 25
4ATO_G - 0.38 0.30 0.50 3 522 3 0 3 0 7
4ENB_A 0.76 0.58 1.00 11 1264 0 0 0 0 8
4ENC_A 0.31 0.26 0.38 5 1313 8 1 7 0 14
4FNJ_A - 0.79 0.63 1.00 10 585 0 0 0 0 6
4FRG_B 0.30 0.22 0.41 7 3469 10 1 9 0 25
4FRN_A 0.45 0.36 0.57 13 5128 10 2 8 0 23
4HXH_A - 1.00 1.00 1.00 6 319 0 0 0 0 0

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.