CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of HotKnots - scored higher in this pairwise comparison

  4. Performance of Vsfold5 - scored lower in this pairwise comparison

  5. Compile and download dataset for HotKnots & Vsfold5 [.zip] - may take several seconds...


Overview

Metric HotKnots Vsfold5
MCC 0.639 > 0.510
Average MCC ± 95% Confidence Intervals 0.703 ± 0.049 > 0.601 ± 0.058
Sensitivity 0.575 > 0.441
Positive Predictive Value 0.715 > 0.596
Total TP 1847 > 1415
Total TN 465982 < 466193
Total FP 857 < 1045
Total FP CONTRA 103 < 110
Total FP INCONS 635 < 849
Total FP COMP 119 > 86
Total FN 1363 < 1795
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of HotKnots and Vsfold5. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for HotKnots and Vsfold5).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for HotKnots and Vsfold5).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for HotKnots and Vsfold5. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for HotKnots and Vsfold5).

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Performance of HotKnots - scored higher in this pairwise comparison

1. Total counts & total scores for HotKnots

Total Base Pair Counts
Total TP 1847
Total TN 465982
Total FP 857
Total FP CONTRA 103
Total FP INCONS 635
Total FP COMP 119
Total FN 1363
Total Scores
MCC 0.639
Average MCC ± 95% Confidence Intervals 0.703 ± 0.049
Sensitivity 0.575
Positive Predictive Value 0.715
Nr of predictions 127

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2. Individual counts for HotKnots [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 395 0 0 0 0 0
2K63_A - 0.95 0.90 1.00 9 397 0 0 0 0 1
2K66_A - 0.95 0.90 1.00 9 222 0 0 0 0 1
2KD8_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
2KDQ_B 0.95 0.91 1.00 10 396 0 0 0 0 1
2KE6_A 0.97 0.95 1.00 18 1110 1 0 0 1 1
2KEZ_A - 0.89 0.80 1.00 8 268 0 0 0 0 2
2KFC_A - -0.02 0.00 0.00 0 623 7 0 7 0 20
2KGP_A - 1.00 1.00 1.00 9 291 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 367 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 549 0 0 0 0 1
2KRL_A - 0.76 0.60 0.96 24 5126 5 0 1 4 16
2KU0_A - 0.83 0.69 1.00 11 692 0 0 0 0 5
2KUR_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KUU_A 0.92 0.86 1.00 18 1110 1 0 0 1 3
2KUV_A 0.93 0.86 1.00 19 1109 0 0 0 0 3
2KUW_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KX8_A 0.94 0.89 1.00 16 845 0 0 0 0 2
2KXM_A - 0.89 0.80 1.00 8 343 0 0 0 0 2
2KZL_A - 0.86 0.74 1.00 14 1471 0 0 0 0 5
2L1F_A 0.98 0.96 1.00 23 2057 0 0 0 0 1
2L1F_B 0.98 0.96 1.00 24 2121 0 0 0 0 1
2L2K_A - 0.94 0.89 1.00 17 844 0 0 0 0 2
2L3C_B - 0.91 0.82 1.00 14 547 0 0 0 0 3
2L3E_A - 0.96 0.92 1.00 12 583 1 0 0 1 1
2L3J_B 0.94 0.88 1.00 30 2455 0 0 0 0 4
2L5Z_A - 0.86 0.75 1.00 9 316 0 0 0 0 3
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - 0.39 0.26 0.63 5 622 3 1 2 0 14
2LBS_A - 1.00 1.00 1.00 14 482 0 0 0 0 0
2LC8_A 0.61 0.55 0.69 11 1524 5 0 5 0 9
2LDL_A - 0.90 0.82 1.00 9 342 1 0 0 1 2
2LDT_A - 0.85 0.73 1.00 11 454 0 0 0 0 4
2LHP_A - 0.97 0.94 1.00 15 651 0 0 0 0 1
2LI4_A - 0.93 0.88 1.00 14 482 0 0 0 0 2
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 0.95 0.90 1.00 9 267 0 0 0 0 1
2LKR_A - 0.93 0.87 1.00 34 6071 3 0 0 3 5
2LWK_A - 0.83 0.77 0.91 10 485 1 0 1 0 3
2RP0_A - 0.64 0.42 1.00 5 346 0 0 0 0 7
2RPT_A - 1.00 1.00 1.00 7 183 0 0 0 0 0
2RRC_A - 0.83 0.70 1.00 7 224 0 0 0 0 3
2WRQ_Y 0.57 0.59 0.56 10 2832 13 5 3 5 7
2WW9_E - 0.53 0.29 1.00 4 557 0 0 0 0 10
2WW9_D - 0.14 0.12 0.19 3 1937 13 0 13 0 23
2WW9_F - 0.59 0.60 0.60 6 290 4 0 4 0 4
2WWQ_V 0.76 0.68 0.86 19 2904 5 0 3 2 9
2XKV_B 0.51 0.50 0.53 10 4541 23 0 9 14 10
2XQD_Y 0.88 0.78 1.00 21 2829 0 0 0 0 6
2XXA_G 0.34 0.31 0.39 13 5118 20 1 19 0 29
2Y8W_B - 1.00 1.00 1.00 6 184 1 0 0 1 0
2Y9C_V - 0.65 0.63 0.68 15 2256 8 1 6 1 9
2YIE_X - 0.36 0.33 0.40 4 1368 6 2 4 0 8
2YIE_Z - -0.01 0.00 0.00 0 1525 15 3 12 0 12
2ZY6_A - 0.58 0.58 0.58 7 691 5 1 4 0 5
2ZZM_B 0.21 0.19 0.25 6 3462 18 0 18 0 26
2ZZN_D 0.82 0.78 0.88 21 2461 3 0 3 0 6
3A2K_C 0.42 0.39 0.46 11 2902 13 2 11 0 17
3A3A_A 0.87 0.76 1.00 28 3627 0 0 0 0 9
3ADB_C - 0.79 0.74 0.85 28 4153 5 0 5 0 10
3AKZ_H 0.43 0.39 0.48 11 2678 12 2 10 0 17
3AM1_B - 0.78 0.71 0.86 25 3211 4 0 4 0 10
3AMU_B 0.70 0.59 0.84 16 2984 5 0 3 2 11
3DW4_A - -0.02 0.00 0.00 0 345 6 0 6 0 10
3G4S_9 0.50 0.39 0.65 22 7347 12 1 11 0 35
3GCA_A - 0.54 0.29 1.00 5 523 0 0 0 0 12
3GX2_A 0.68 0.55 0.85 22 4345 5 0 4 1 18
3HAY_E - 0.91 0.83 1.00 15 2263 6 0 0 6 3
3HJW_D - 0.94 0.89 1.00 16 1637 2 0 0 2 2
3IAB_R - 0.92 0.86 1.00 12 1023 0 0 0 0 2
3ID5_D - 0.44 0.33 0.60 3 491 5 0 2 3 6
3IVN_B 0.78 0.61 1.00 19 2327 0 0 0 0 12
3IWN_A 0.63 0.58 0.70 19 4251 9 0 8 1 14
3IYQ_A 0.31 0.34 0.29 32 60617 81 22 55 4 62
3IZ4_A 0.51 0.47 0.55 62 70763 52 10 41 1 70
3IZF_C 0.70 0.61 0.80 33 6862 8 1 7 0 21
3J0L_8 - 0.93 0.88 1.00 7 183 0 0 0 0 1
3J0L_1 - 0.76 0.63 0.92 12 1212 2 0 1 1 7
3J0L_h - 0.75 0.60 0.93 26 6077 4 0 2 2 17
3J0L_2 - 0.28 0.27 0.29 9 6185 23 3 19 1 24
3J0L_7 - 0.28 0.29 0.29 5 1208 12 0 12 0 12
3J0L_g - -0.01 0.00 0.00 0 460 5 1 4 0 4
3J0L_a - 0.51 0.50 0.53 8 1113 7 3 4 0 8
3J16_L 0.26 0.23 0.30 7 2752 16 1 15 0 23
3J20_0 0.45 0.40 0.52 12 2827 12 0 11 1 18
3J20_1 0.73 0.70 0.76 16 2905 7 0 5 2 7
3J2C_O - 0.60 0.49 0.74 31 10254 12 0 11 1 32
3J2L_3 0.62 0.53 0.74 28 7837 12 0 10 2 25
3JYV_7 -0.01 0.00 0.00 0 2828 22 1 21 0 32
3JYX_4 0.32 0.30 0.33 10 12216 31 5 15 11 23
3JYX_3 0.62 0.63 0.61 17 6300 22 1 10 11 10
3KTW_C - 0.73 0.63 0.84 27 4528 6 2 3 1 16
3LA5_A 0.78 0.62 1.00 21 2464 0 0 0 0 13
3NDB_M - 0.84 0.74 0.96 45 9133 3 0 2 1 16
3NKB_B - 0.59 0.54 0.67 14 1995 7 0 7 0 12
3NMU_E - 0.54 0.50 0.60 3 556 5 0 2 3 3
3NPB_A 0.78 0.65 0.94 30 6989 5 0 2 3 16
3O58_3 0.23 0.26 0.21 9 12360 34 10 24 0 26
3O58_2 0.71 0.71 0.71 27 7222 12 4 7 1 11
3OVB_D - 1.00 1.00 1.00 11 584 1 0 0 1 0
3OVS_D - 0.96 0.92 1.00 12 549 0 0 0 0 1
3P22_G - 0.96 0.92 1.00 11 730 0 0 0 0 1
3PDR_A 0.67 0.56 0.82 40 12831 11 0 9 2 32
3R4F_A - 0.96 0.92 1.00 23 2122 0 0 0 0 2
3R9X_C - 0.84 0.80 0.89 8 586 2 0 1 1 2
3RKF_A 0.76 0.59 1.00 20 2191 0 0 0 0 14
3SD1_A 0.64 0.52 0.79 22 3888 6 1 5 0 20
3SIU_F - 0.85 0.73 1.00 8 370 0 0 0 0 3
3SN2_B 0.96 0.92 1.00 11 395 0 0 0 0 1
3TRZ_Z - 0.91 0.83 1.00 5 205 1 0 0 1 1
3TS0_U - 1.00 1.00 1.00 6 247 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 271 0 0 0 0 0
3U4M_B - 0.38 0.32 0.46 12 3134 14 0 14 0 25
3UZL_B 0.48 0.38 0.61 14 3547 9 0 9 0 23
3VJR_D - 0.96 0.92 1.00 12 618 0 0 0 0 1
3W3S_B 0.55 0.50 0.61 20 4720 14 1 12 1 20
3ZEX_D 0.78 0.67 0.92 33 6985 3 0 3 0 16
3ZEX_E - 0.00 0.00 0.00 0 21893 54 4 48 2 77
4A1C_3 0.70 0.59 0.82 32 7101 7 1 6 0 22
4A1C_2 0.14 0.15 0.14 5 11745 42 6 25 11 28
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4AOB_A 0.50 0.40 0.63 17 4344 11 2 8 1 25
4ATO_G - 0.61 0.70 0.54 7 515 6 2 4 0 3
4ENB_A 0.89 0.79 1.00 15 1260 0 0 0 0 4
4ENC_A 0.86 0.79 0.94 15 1310 1 1 0 0 4
4FRG_B 0.32 0.28 0.38 9 3462 15 0 15 0 23
4FRN_A 0.52 0.44 0.62 16 5125 10 2 8 0 20

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Performance of Vsfold5 - scored lower in this pairwise comparison

1. Total counts & total scores for Vsfold5

Total Base Pair Counts
Total TP 1415
Total TN 466193
Total FP 1045
Total FP CONTRA 110
Total FP INCONS 849
Total FP COMP 86
Total FN 1795
Total Scores
MCC 0.510
Average MCC ± 95% Confidence Intervals 0.601 ± 0.058
Sensitivity 0.441
Positive Predictive Value 0.596
Nr of predictions 127

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2. Individual counts for Vsfold5 [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 395 0 0 0 0 0
2K63_A - 0.95 0.90 1.00 9 397 0 0 0 0 1
2K66_A - 0.95 0.90 1.00 9 222 0 0 0 0 1
2KD8_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
2KDQ_B 0.90 0.82 1.00 9 397 0 0 0 0 2
2KE6_A 0.97 0.95 1.00 18 1110 1 0 0 1 1
2KEZ_A - 0.70 0.50 1.00 5 271 0 0 0 0 5
2KFC_A - 0.48 0.35 0.70 7 620 3 0 3 0 13
2KGP_A - 0.81 0.67 1.00 6 294 1 0 0 1 3
2KMJ_A - 0.95 0.91 1.00 10 368 0 0 0 0 1
2KPV_A - 0.96 0.92 1.00 12 549 0 0 0 0 1
2KRL_A - 0.57 0.50 0.65 20 5120 11 5 6 0 20
2KU0_A - -0.02 0.00 0.00 0 692 11 0 11 0 16
2KUR_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KUU_A 0.92 0.86 1.00 18 1110 1 0 0 1 3
2KUV_A 0.93 0.86 1.00 19 1109 0 0 0 0 3
2KUW_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KX8_A 0.91 0.83 1.00 15 846 0 0 0 0 3
2KXM_A - 0.70 0.50 1.00 5 346 0 0 0 0 5
2KZL_A - 0.86 0.74 1.00 14 1471 0 0 0 0 5
2L1F_A 0.69 0.58 0.82 14 2063 3 0 3 0 10
2L1F_B 0.70 0.60 0.83 15 2127 3 0 3 0 10
2L2K_A - 0.89 0.79 1.00 15 846 0 0 0 0 4
2L3C_B - 0.91 0.82 1.00 14 547 0 0 0 0 3
2L3E_A - 0.96 0.92 1.00 12 583 1 0 0 1 1
2L3J_B 0.87 0.76 1.00 26 2459 0 0 0 0 8
2L5Z_A - 0.60 0.50 0.75 6 317 2 0 2 0 6
2L94_A 0.95 0.90 1.00 18 972 0 0 0 0 2
2LA5_A - -0.02 0.00 0.00 0 623 7 0 7 0 19
2LBS_A - 1.00 1.00 1.00 14 482 0 0 0 0 0
2LC8_A -0.01 0.00 0.00 0 1527 13 0 13 0 20
2LDL_A - 0.85 0.73 1.00 8 343 1 0 0 1 3
2LDT_A - 0.85 0.73 1.00 11 454 0 0 0 0 4
2LHP_A - 0.97 0.94 1.00 15 651 0 0 0 0 1
2LI4_A - 0.93 0.88 1.00 14 482 0 0 0 0 2
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 0.89 0.80 1.00 8 268 0 0 0 0 2
2LKR_A - 0.27 0.23 0.32 9 6077 19 2 17 0 30
2LWK_A - 0.83 0.77 0.91 10 485 1 0 1 0 3
2RP0_A - 0.64 0.42 1.00 5 346 0 0 0 0 7
2RPT_A - 0.70 0.71 0.71 5 183 2 0 2 0 2
2RRC_A - 0.70 0.50 1.00 5 226 0 0 0 0 5
2WRQ_Y 0.53 0.53 0.53 9 2833 12 5 3 4 8
2WW9_E - -0.01 0.00 0.00 0 556 5 0 5 0 14
2WW9_D - 0.15 0.12 0.21 3 1939 11 1 10 0 23
2WW9_F - 0.59 0.60 0.60 6 290 4 0 4 0 4
2WWQ_V 0.64 0.50 0.82 14 2909 4 0 3 1 14
2XKV_B -0.01 0.00 0.00 0 4528 32 12 20 0 20
2XQD_Y 0.86 0.74 1.00 20 2830 0 0 0 0 7
2XXA_G -0.01 0.00 0.00 0 5120 31 2 29 0 42
2Y8W_B - 1.00 1.00 1.00 6 184 1 0 0 1 0
2Y9C_V - 0.61 0.54 0.68 13 2259 7 1 5 1 11
2YIE_X - 0.30 0.33 0.29 4 1364 10 2 8 0 8
2YIE_Z - -0.01 0.00 0.00 0 1529 11 3 8 0 12
2ZY6_A - 0.58 0.58 0.58 7 691 5 1 4 0 5
2ZZM_B 0.19 0.19 0.21 6 3458 22 1 21 0 26
2ZZN_D 0.86 0.78 0.95 21 2463 1 0 1 0 6
3A2K_C 0.84 0.71 1.00 20 2906 0 0 0 0 8
3A3A_A 0.85 0.73 1.00 27 3628 0 0 0 0 10
3ADB_C - 0.67 0.53 0.87 20 4163 3 0 3 0 18
3AKZ_H 0.84 0.71 1.00 20 2681 0 0 0 0 8
3AM1_B - 0.78 0.71 0.86 25 3211 4 1 3 0 10
3AMU_B 0.67 0.59 0.76 16 2982 7 0 5 2 11
3DW4_A - 0.70 0.50 1.00 5 346 0 0 0 0 5
3G4S_9 0.08 0.07 0.11 4 7344 33 0 33 0 53
3GCA_A - 0.54 0.29 1.00 5 523 0 0 0 0 12
3GX2_A 0.51 0.40 0.67 16 4347 9 0 8 1 24
3HAY_E - 0.91 0.83 1.00 15 2263 3 0 0 3 3
3HJW_D - 0.48 0.50 0.47 9 1634 10 0 10 0 9
3IAB_R - 0.92 0.86 1.00 12 1023 0 0 0 0 2
3ID5_D - 0.44 0.33 0.60 3 491 4 0 2 2 6
3IVN_B 0.76 0.58 1.00 18 2328 0 0 0 0 13
3IWN_A 0.25 0.21 0.30 7 4255 16 1 15 0 26
3IYQ_A 0.33 0.31 0.35 29 60642 61 15 40 6 65
3IZ4_A 0.29 0.24 0.36 32 70787 62 8 49 5 100
3IZF_C 0.70 0.56 0.88 30 6869 4 0 4 0 24
3J0L_8 - 0.70 0.50 1.00 4 186 0 0 0 0 4
3J0L_1 - 0.27 0.21 0.36 4 1214 7 1 6 0 15
3J0L_h - 0.18 0.14 0.24 6 6080 19 1 18 0 37
3J0L_2 - 0.35 0.33 0.37 11 6186 21 3 16 2 22
3J0L_7 - -0.01 0.00 0.00 0 1212 13 0 13 0 17
3J0L_g - -0.01 0.00 0.00 0 461 4 1 3 0 4
3J0L_a - 0.39 0.31 0.50 5 1118 5 2 3 0 11
3J16_L 0.82 0.67 1.00 20 2755 0 0 0 0 10
3J20_0 0.67 0.57 0.81 17 2829 5 1 3 1 13
3J20_1 0.71 0.70 0.73 16 2904 6 0 6 0 7
3J2C_O - 0.30 0.24 0.39 15 10258 24 0 23 1 48
3J2L_3 0.56 0.45 0.71 24 7841 13 1 9 3 29
3JYV_7 -0.01 0.00 0.00 0 2831 19 0 19 0 32
3JYX_4 0.31 0.30 0.31 10 12214 32 3 19 10 23
3JYX_3 0.17 0.15 0.20 4 6308 18 4 12 2 23
3KTW_C - 0.38 0.30 0.50 13 4534 13 3 10 0 30
3LA5_A -0.01 0.00 0.00 0 2469 16 0 16 0 34
3NDB_M - 0.06 0.05 0.08 3 9144 33 1 32 0 58
3NKB_B - 0.59 0.54 0.67 14 1995 7 0 7 0 12
3NMU_E - 0.20 0.17 0.25 1 557 5 0 3 2 5
3NPB_A 0.57 0.46 0.72 21 6992 9 1 7 1 25
3O58_3 0.39 0.37 0.41 13 12371 28 7 12 9 22
3O58_2 0.45 0.42 0.48 16 7227 19 3 14 2 22
3OVB_D - 1.00 1.00 1.00 11 584 1 0 0 1 0
3OVS_D - 0.96 0.92 1.00 12 549 0 0 0 0 1
3P22_G - 0.96 0.92 1.00 11 730 0 0 0 0 1
3PDR_A 0.57 0.44 0.74 32 12837 13 0 11 2 40
3R4F_A - 0.94 0.88 1.00 22 2123 0 0 0 0 3
3R9X_C - 0.84 0.80 0.89 8 586 2 0 1 1 2
3RKF_A 0.75 0.65 0.88 22 2186 3 0 3 0 12
3SD1_A 0.11 0.10 0.15 4 3890 22 0 22 0 38
3SIU_F - 0.73 0.55 1.00 6 372 0 0 0 0 5
3SN2_B 0.96 0.92 1.00 11 395 0 0 0 0 1
3TRZ_Z - 0.91 0.83 1.00 5 205 1 0 0 1 1
3TS0_U - 1.00 1.00 1.00 6 247 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 271 0 0 0 0 0
3U4M_B - 0.38 0.32 0.46 12 3134 14 0 14 0 25
3UZL_B 0.16 0.14 0.20 5 3545 20 1 19 0 32
3VJR_D - 0.96 0.92 1.00 12 618 0 0 0 0 1
3W3S_B 0.76 0.70 0.82 28 4719 7 1 5 1 12
3ZEX_D 0.08 0.06 0.11 3 6994 24 0 24 0 46
3ZEX_E - 0.07 0.06 0.08 5 21886 54 5 49 0 72
4A1C_3 0.26 0.22 0.32 12 7103 25 2 23 0 42
4A1C_2 0.24 0.24 0.24 8 11748 37 3 22 12 25
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4AOB_A 0.18 0.14 0.25 6 4347 19 1 17 1 36
4ATO_G - 0.56 0.60 0.55 6 517 5 1 4 0 4
4ENB_A 0.89 0.79 1.00 15 1260 0 0 0 0 4
4ENC_A 0.86 0.79 0.94 15 1310 1 1 0 0 4
4FRG_B 0.56 0.47 0.68 15 3464 7 1 6 0 17
4FRN_A 0.13 0.11 0.17 4 5128 19 2 17 0 32

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.