CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of PknotsRG - scored higher in this pairwise comparison

  4. Performance of MCFold - scored lower in this pairwise comparison

  5. Compile and download dataset for PknotsRG & MCFold [.zip] - may take several seconds...


Overview

Metric PknotsRG MCFold
MCC 0.643 > 0.522
Average MCC ± 95% Confidence Intervals 0.692 ± 0.049 > 0.599 ± 0.061
Sensitivity 0.580 > 0.535
Positive Predictive Value 0.718 > 0.516
Total TP 1745 > 1611
Total TN 350206 > 349515
Total FP 836 < 1767
Total FP CONTRA 81 < 194
Total FP INCONS 604 < 1316
Total FP COMP 151 < 257
Total FN 1264 < 1398
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of PknotsRG and MCFold. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for PknotsRG and MCFold).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for PknotsRG and MCFold).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for PknotsRG and MCFold. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for PknotsRG and MCFold).

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Performance of PknotsRG - scored higher in this pairwise comparison

1. Total counts & total scores for PknotsRG

Total Base Pair Counts
Total TP 1745
Total TN 350206
Total FP 836
Total FP CONTRA 81
Total FP INCONS 604
Total FP COMP 151
Total FN 1264
Total Scores
MCC 0.643
Average MCC ± 95% Confidence Intervals 0.692 ± 0.049
Sensitivity 0.580
Positive Predictive Value 0.718
Nr of predictions 131

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2. Individual counts for PknotsRG [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 395 0 0 0 0 0
2K63_A - 0.95 0.90 1.00 9 397 0 0 0 0 1
2K66_A - 0.95 0.90 1.00 9 222 0 0 0 0 1
2KD8_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
2KDQ_B 0.95 0.91 1.00 10 396 0 0 0 0 1
2KE6_A 0.86 0.84 0.89 16 1110 3 0 2 1 3
2KEZ_A - 0.89 0.80 1.00 8 268 0 0 0 0 2
2KFC_A - 0.48 0.35 0.70 7 620 3 0 3 0 13
2KGP_A - 0.70 0.67 0.75 6 292 2 0 2 0 3
2KMJ_A - 1.00 1.00 1.00 11 367 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 549 0 0 0 0 1
2KU0_A - 0.83 0.69 1.00 11 692 0 0 0 0 5
2KUR_A 0.85 0.81 0.89 17 1109 2 0 2 0 4
2KUU_A 0.82 0.76 0.89 16 1110 3 0 2 1 5
2KUV_A 0.83 0.77 0.89 17 1109 2 0 2 0 5
2KUW_A 0.90 0.86 0.95 18 1109 1 0 1 0 3
2KX8_A 0.94 0.89 1.00 16 845 0 0 0 0 2
2KXM_A - 0.83 0.70 1.00 7 344 0 0 0 0 3
2KZL_A - 0.86 0.74 1.00 14 1471 0 0 0 0 5
2L1F_A 0.98 0.96 1.00 23 2057 0 0 0 0 1
2L1F_B 0.98 0.96 1.00 24 2121 0 0 0 0 1
2L2K_A - 0.94 0.89 1.00 17 844 0 0 0 0 2
2L3C_B - 0.91 0.82 1.00 14 547 0 0 0 0 3
2L3E_A - 0.96 0.92 1.00 12 583 1 0 0 1 1
2L3J_B 0.94 0.88 1.00 30 2455 0 0 0 0 4
2L5Z_A - 0.86 0.75 1.00 9 316 0 0 0 0 3
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - 0.39 0.26 0.63 5 622 3 1 2 0 14
2LBS_A - 1.00 1.00 1.00 14 482 0 0 0 0 0
2LC8_A 0.63 0.55 0.73 11 1525 4 1 3 0 9
2LDL_A - 0.90 0.82 1.00 9 342 1 0 0 1 2
2LDT_A - 0.85 0.73 1.00 11 454 0 0 0 0 4
2LHP_A - 0.97 0.94 1.00 15 651 0 0 0 0 1
2LI4_A - 0.93 0.88 1.00 14 482 0 0 0 0 2
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 0.95 0.90 1.00 9 267 0 0 0 0 1
2LKR_A - 0.73 0.69 0.77 27 6070 8 2 6 0 12
2LQZ_A - 0.85 0.82 0.90 9 341 1 1 0 0 2
2LU0_A - 1.00 1.00 1.00 16 1160 0 0 0 0 0
2LWK_A - 0.83 0.77 0.91 10 485 1 0 1 0 3
2M58_A - 0.81 0.71 0.92 12 1640 1 1 0 0 5
2RP0_A - 0.66 0.58 0.78 7 342 2 1 1 0 5
2RPT_A - 1.00 1.00 1.00 7 183 0 0 0 0 0
2RRC_A - 0.70 0.50 1.00 5 226 0 0 0 0 5
2WRQ_Y 0.53 0.53 0.53 9 2833 13 5 3 5 8
2WW9_F - 0.69 0.70 0.70 7 290 3 0 3 0 3
2WW9_D - 0.14 0.12 0.19 3 1937 13 0 13 0 23
2WW9_E - 0.53 0.29 1.00 4 557 0 0 0 0 10
2WWQ_V 0.76 0.68 0.86 19 2904 5 0 3 2 9
2XKV_B 0.51 0.50 0.53 10 4541 22 0 9 13 10
2XQD_Y 0.88 0.78 1.00 21 2829 0 0 0 0 6
2XXA_G 0.34 0.31 0.39 13 5118 20 1 19 0 29
2Y8W_B - 1.00 1.00 1.00 6 184 1 0 0 1 0
2Y9C_V - 0.66 0.63 0.71 15 2257 8 1 5 2 9
2YIE_Z - -0.01 0.00 0.00 0 1525 15 3 12 0 12
2YIE_X - 0.30 0.33 0.29 4 1364 10 2 8 0 8
2ZY6_A - 0.58 0.58 0.58 7 691 5 1 4 0 5
2ZZN_D 0.84 0.78 0.91 21 2462 2 0 2 0 6
3A2K_C 0.42 0.39 0.46 11 2902 13 2 11 0 17
3A3A_A 0.87 0.76 1.00 28 3627 0 0 0 0 9
3AKZ_H 0.41 0.39 0.44 11 2676 14 4 10 0 17
3AM1_B - 0.68 0.63 0.73 22 3210 8 1 7 0 13
3AMU_B 0.70 0.59 0.84 16 2984 5 0 3 2 11
3DW4_A - -0.02 0.00 0.00 0 348 3 0 3 0 10
3GCA_A - 0.54 0.29 1.00 5 523 0 0 0 0 12
3GX2_A 0.67 0.55 0.81 22 4344 6 0 5 1 18
3HAY_E - 0.91 0.83 1.00 15 2263 6 0 0 6 3
3HJW_D - 0.94 0.89 1.00 16 1637 2 0 0 2 2
3IAB_R - 0.92 0.86 1.00 12 1023 0 0 0 0 2
3ID5_D - 0.40 0.33 0.50 3 490 6 0 3 3 6
3IVN_B 0.78 0.61 1.00 19 2327 0 0 0 0 12
3IWN_A 0.20 0.18 0.24 6 4253 19 1 18 0 27
3IZF_C 0.59 0.52 0.67 28 6861 14 1 13 0 26
3J0L_8 - 0.93 0.88 1.00 7 183 0 0 0 0 1
3J0L_2 - 0.24 0.24 0.24 8 6183 27 2 23 2 25
3J0L_g - -0.01 0.00 0.00 0 461 4 1 3 0 4
3J0L_7 - 0.28 0.29 0.29 5 1208 12 0 12 0 12
3J0L_h - 0.75 0.60 0.93 26 6077 4 0 2 2 17
3J0L_1 - 0.76 0.63 0.92 12 1212 2 0 1 1 7
3J0L_a - 0.23 0.19 0.30 3 1118 7 1 6 0 13
3J16_L 0.75 0.57 1.00 17 2758 0 0 0 0 13
3J20_0 0.43 0.40 0.48 12 2825 14 0 13 1 18
3J2L_3 0.62 0.53 0.74 28 7837 12 0 10 2 25
3J3D_C 0.60 0.54 0.68 15 2753 7 0 7 0 13
3J3E_8 0.17 0.15 0.19 5 7477 30 2 19 9 28
3J3E_7 0.58 0.48 0.70 26 7103 11 1 10 0 28
3J3F_7 0.80 0.70 0.92 35 7222 4 0 3 1 15
3J3F_8 0.31 0.31 0.31 11 12211 37 4 20 13 25
3JYV_7 -0.01 0.00 0.00 0 2828 22 1 21 0 32
3JYX_3 0.62 0.63 0.61 17 6300 22 1 10 11 10
3JYX_4 0.31 0.30 0.32 10 12215 33 5 16 12 23
3KTW_C - 0.77 0.65 0.90 28 4529 4 2 1 1 15
3LA5_A 0.78 0.62 1.00 21 2464 0 0 0 0 13
3NDB_M - 0.77 0.69 0.86 42 9131 8 0 7 1 19
3NKB_B - 0.59 0.54 0.67 14 1995 7 0 7 0 12
3NMU_E - 0.54 0.50 0.60 3 556 5 0 2 3 3
3O58_3 0.34 0.34 0.34 12 12368 34 2 21 11 23
3O58_2 0.59 0.61 0.58 23 7220 19 4 13 2 15
3OVB_D - 1.00 1.00 1.00 11 584 1 0 0 1 0
3OVS_D - 0.96 0.92 1.00 12 549 0 0 0 0 1
3P22_G - 0.96 0.92 1.00 11 730 0 0 0 0 1
3PDR_A 0.75 0.63 0.90 45 12830 7 1 4 2 27
3R4F_A - 0.96 0.92 1.00 23 2122 0 0 0 0 2
3R9X_C - 0.84 0.80 0.89 8 586 2 0 1 1 2
3RKF_A 0.76 0.59 1.00 20 2191 0 0 0 0 14
3SD1_A 0.61 0.50 0.75 21 3888 7 1 6 0 21
3SIU_F - 0.85 0.73 1.00 8 370 0 0 0 0 3
3SN2_B 0.96 0.92 1.00 11 395 0 0 0 0 1
3TRZ_Z - 0.91 0.83 1.00 5 205 1 0 0 1 1
3TS0_U - 1.00 1.00 1.00 6 247 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 271 0 0 0 0 0
3U4M_B - 0.38 0.32 0.46 12 3134 14 0 14 0 25
3VJR_D - 0.96 0.92 1.00 12 618 0 0 0 0 1
3ZEX_G - 0.56 0.46 0.68 34 16421 21 1 15 5 40
3ZEX_F - 0.00 0.00 0.00 0 2617 11 2 9 0 12
3ZEX_C 0.24 0.21 0.28 11 14157 43 1 27 15 41
3ZEX_H - 0.18 0.18 0.18 7 9006 32 6 26 0 31
3ZEX_D 0.77 0.65 0.91 32 6986 3 0 3 0 17
3ZND_W 0.20 0.22 0.19 5 2977 22 1 20 1 18
4A1C_2 0.14 0.15 0.13 5 11742 43 5 29 9 28
4A1C_3 0.70 0.59 0.82 32 7101 7 1 6 0 22
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4ATO_G - 0.73 0.70 0.78 7 519 2 1 1 0 3
4ENB_A 0.89 0.79 1.00 15 1260 0 0 0 0 4
4ENC_A 0.86 0.79 0.94 15 1310 1 1 0 0 4
4FNJ_A - 0.79 0.63 1.00 10 585 0 0 0 0 6
4FRG_B 0.32 0.28 0.38 9 3462 15 0 15 0 23
4FRN_A 0.52 0.44 0.62 16 5125 10 2 8 0 20
4HXH_A - 1.00 1.00 1.00 6 319 0 0 0 0 0
4JF2_A 0.77 0.71 0.85 22 2824 4 3 1 0 9
4JRC_A - 0.29 0.26 0.35 6 1523 11 0 11 0 17

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Performance of MCFold - scored lower in this pairwise comparison

1. Total counts & total scores for MCFold

Total Base Pair Counts
Total TP 1611
Total TN 349515
Total FP 1767
Total FP CONTRA 194
Total FP INCONS 1316
Total FP COMP 257
Total FN 1398
Total Scores
MCC 0.522
Average MCC ± 95% Confidence Intervals 0.599 ± 0.061
Sensitivity 0.535
Positive Predictive Value 0.516
Nr of predictions 131

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2. Individual counts for MCFold [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 395 1 0 0 1 0
2K63_A - 0.90 0.90 0.90 9 396 4 0 1 3 1
2K66_A - 1.00 1.00 1.00 10 221 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 222 1 0 0 1 0
2KDQ_B 0.95 0.91 1.00 10 396 1 0 0 1 1
2KE6_A 1.00 1.00 1.00 19 1109 2 0 0 2 0
2KEZ_A - 0.90 0.90 0.90 9 266 1 0 1 0 1
2KFC_A - 0.28 0.25 0.36 5 616 9 2 7 0 15
2KGP_A - 1.00 1.00 1.00 9 291 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 367 1 0 0 1 0
2KPV_A - 1.00 1.00 1.00 13 548 2 0 0 2 0
2KU0_A - 0.97 0.94 1.00 15 688 0 0 0 0 1
2KUR_A 1.00 1.00 1.00 21 1107 0 0 0 0 0
2KUU_A 0.98 0.95 1.00 20 1108 1 0 0 1 1
2KUV_A 0.98 0.95 1.00 21 1107 0 0 0 0 1
2KUW_A 0.95 0.95 0.95 20 1107 1 0 1 0 1
2KX8_A 0.91 0.89 0.94 16 844 2 0 1 1 2
2KXM_A - 1.00 1.00 1.00 10 341 1 0 0 1 0
2KZL_A - 0.80 0.84 0.76 16 1464 9 1 4 4 3
2L1F_B 0.73 0.76 0.70 19 2118 9 0 8 1 6
2L1F_A 0.72 0.75 0.69 18 2054 9 0 8 1 6
2L2K_A - 1.00 1.00 1.00 19 842 1 0 0 1 0
2L3C_B - 0.97 0.94 1.00 16 545 0 0 0 0 1
2L3E_A - 1.00 1.00 1.00 13 582 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 34 2451 0 0 0 0 0
2L5Z_A - 0.96 0.92 1.00 11 314 0 0 0 0 1
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - -0.03 0.00 0.00 0 615 15 0 15 0 19
2LBS_A - 1.00 1.00 1.00 14 482 1 0 0 1 0
2LC8_A 0.41 0.45 0.39 9 1517 16 0 14 2 11
2LDL_A - 0.95 0.91 1.00 10 341 2 0 0 2 1
2LDT_A - 0.75 0.73 0.79 11 451 3 0 3 0 4
2LHP_A - 1.00 1.00 1.00 16 650 1 0 0 1 0
2LI4_A - 0.97 0.94 1.00 15 481 0 0 0 0 1
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 1.00 1.00 1.00 10 266 0 0 0 0 0
2LKR_A - 0.93 0.92 0.95 36 6067 14 0 2 12 3
2LQZ_A - 0.91 0.91 0.91 10 340 1 1 0 0 1
2LU0_A - 1.00 1.00 1.00 16 1160 5 0 0 5 0
2LWK_A - 0.92 0.92 0.92 12 483 2 0 1 1 1
2M58_A - 0.20 0.24 0.18 4 1631 19 2 16 1 13
2RP0_A - 0.44 0.42 0.50 5 341 5 1 4 0 7
2RPT_A - 1.00 1.00 1.00 7 183 2 0 0 2 0
2RRC_A - 0.95 0.90 1.00 9 222 0 0 0 0 1
2WRQ_Y 0.27 0.35 0.22 6 2823 25 8 13 4 11
2WW9_F - 0.59 0.60 0.60 6 290 4 0 4 0 4
2WW9_D - -0.01 0.00 0.00 0 1925 28 5 23 0 26
2WW9_E - -0.02 0.00 0.00 0 547 14 0 14 0 14
2WWQ_V 0.15 0.18 0.15 5 2892 30 4 25 1 23
2XKV_B 0.20 0.25 0.17 5 4530 37 2 23 12 15
2XQD_Y 0.34 0.37 0.32 10 2819 22 1 20 1 17
2XXA_G 0.23 0.24 0.24 10 5110 32 1 30 1 32
2Y8W_B - 1.00 1.00 1.00 6 184 2 0 0 2 0
2Y9C_V - 0.62 0.67 0.59 16 2251 13 2 9 2 8
2YIE_X - 0.06 0.08 0.06 1 1361 21 1 15 5 11
2YIE_Z - 0.36 0.50 0.27 6 1518 18 4 12 2 6
2ZY6_A - 0.51 0.58 0.47 7 688 8 3 5 0 5
2ZZN_D 0.61 0.63 0.61 17 2457 13 1 10 2 10
3A2K_C 0.44 0.46 0.42 13 2895 18 2 16 0 15
3A3A_A 0.89 0.86 0.91 32 3620 3 1 2 0 5
3AKZ_H 0.40 0.43 0.39 12 2670 19 3 16 0 16
3AM1_B - 0.93 0.89 0.97 31 3208 2 0 1 1 4
3AMU_B 0.44 0.48 0.42 13 2972 18 2 16 0 14
3DW4_A - 0.90 0.90 0.90 9 341 3 0 1 2 1
3GCA_A - 0.04 0.06 0.07 1 514 13 1 12 0 16
3GX2_A 0.47 0.48 0.48 19 4331 22 0 21 1 21
3HAY_E - 1.00 1.00 1.00 18 2260 11 0 0 11 0
3HJW_D - 0.94 0.94 0.94 17 1635 8 0 1 7 1
3IAB_R - 0.84 0.93 0.76 13 1018 8 2 2 4 1
3ID5_D - 0.34 0.33 0.38 3 488 9 0 5 4 6
3IVN_B 0.39 0.39 0.40 12 2316 18 0 18 0 19
3IWN_A 0.19 0.21 0.18 7 4238 33 8 25 0 26
3IZF_C 0.71 0.69 0.74 37 6853 14 0 13 1 17
3J0L_g - 0.13 0.25 0.08 1 452 12 8 4 0 3
3J0L_h - 0.91 0.91 0.91 39 6062 13 0 4 9 4
3J0L_7 - -0.01 0.00 0.00 0 1206 19 4 15 0 17
3J0L_2 - 0.21 0.24 0.18 8 6172 39 7 29 3 25
3J0L_8 - 1.00 1.00 1.00 8 182 0 0 0 0 0
3J0L_a - 0.16 0.19 0.17 3 1110 15 1 14 0 13
3J0L_1 - 0.79 0.79 0.79 15 1206 6 0 4 2 4
3J16_L 0.45 0.47 0.44 14 2743 19 1 17 1 16
3J20_0 0.59 0.57 0.63 17 2823 12 1 9 2 13
3J2L_3 0.58 0.57 0.60 30 7825 23 2 18 3 23
3J3D_C 0.42 0.43 0.43 12 2747 18 2 14 2 16
3J3E_8 0.12 0.12 0.12 4 7470 46 3 26 17 29
3J3E_7 0.40 0.39 0.42 21 7090 29 3 26 0 33
3J3F_7 0.74 0.74 0.74 37 7210 17 2 11 4 13
3J3F_8 0.13 0.17 0.11 6 12191 61 9 40 12 30
3JYV_7 0.21 0.22 0.21 7 2817 26 0 26 0 25
3JYX_3 0.41 0.52 0.33 14 6285 34 12 17 5 13
3JYX_4 0.20 0.24 0.17 8 12199 43 16 23 4 25
3KTW_C - 0.40 0.40 0.43 17 4520 24 4 19 1 26
3LA5_A 0.32 0.32 0.34 11 2453 21 1 20 0 23
3NDB_M - 0.23 0.23 0.24 14 9121 46 1 44 1 47
3NKB_B - 0.56 0.58 0.56 15 1989 13 0 12 1 11
3NMU_E - 0.28 0.33 0.25 2 553 12 0 6 6 4
3O58_2 0.20 0.24 0.17 9 7208 44 5 38 1 29
3O58_3 0.22 0.26 0.19 9 12355 45 9 30 6 26
3OVB_D - 1.00 1.00 1.00 11 584 2 0 0 2 0
3OVS_D - 1.00 1.00 1.00 13 548 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 12 729 5 0 0 5 0
3PDR_A 0.66 0.61 0.72 44 12819 19 0 17 2 28
3R4F_A - 0.92 0.92 0.92 23 2120 5 0 2 3 2
3R9X_C - 0.69 0.70 0.70 7 585 7 0 3 4 3
3RKF_A 0.70 0.65 0.76 22 2182 7 1 6 0 12
3SD1_A 0.33 0.33 0.35 14 3876 26 0 26 0 28
3SIU_F - 0.42 0.45 0.42 5 366 7 1 6 0 6
3SN2_B 0.55 0.58 0.54 7 393 6 0 6 0 5
3TRZ_Z - -0.04 0.00 0.00 0 201 9 0 9 0 6
3TS0_U - 1.00 1.00 1.00 6 247 2 0 0 2 0
3TS2_V - -0.02 0.00 0.00 0 268 9 0 8 1 5
3U4M_B - 0.59 0.59 0.59 22 3123 15 0 15 0 15
3VJR_D - 1.00 1.00 1.00 13 617 1 0 0 1 0
3ZEX_H - 0.14 0.18 0.12 7 8987 52 14 37 1 31
3ZEX_G - 0.00 0.00 0.00 0 16456 15 1 14 0 74
3ZEX_D 0.17 0.18 0.18 9 6970 42 4 38 0 40
3ZEX_C 0.24 0.21 0.28 11 14156 29 3 26 0 41
3ZEX_F - -0.01 0.00 0.00 0 2610 29 4 14 11 12
3ZND_W 0.19 0.22 0.18 5 2975 26 1 22 3 18
4A1C_2 0.13 0.15 0.11 5 11735 56 10 31 15 28
4A1C_3 0.68 0.67 0.71 36 7089 17 1 14 2 18
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4ATO_G - 0.30 0.30 0.33 3 519 8 0 6 2 7
4ENB_A 0.61 0.63 0.60 12 1255 8 2 6 0 7
4ENC_A 0.28 0.32 0.27 6 1304 17 2 14 1 13
4FNJ_A - 0.83 0.81 0.87 13 580 3 0 2 1 3
4FRG_B 0.32 0.34 0.31 11 3450 25 0 25 0 21
4FRN_A 0.12 0.14 0.12 5 5110 38 1 35 2 31
4HXH_A - 1.00 1.00 1.00 6 319 4 0 0 4 0
4JF2_A 0.66 0.68 0.66 21 2818 11 0 11 0 10
4JRC_A - 0.20 0.22 0.21 5 1516 19 0 19 0 18

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.