CompaRNA - on-line benchmarks of RNA structure prediction methods
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Table of contents:

  1. Overview

  2. Performance Plots

  3. Performance of RNAshapes - scored higher in this pairwise comparison

  4. Performance of MCFold - scored lower in this pairwise comparison

  5. Compile and download dataset for RNAshapes & MCFold [.zip] - may take several seconds...


Overview

Metric RNAshapes MCFold
MCC 0.638 > 0.522
Average MCC ± 95% Confidence Intervals 0.681 ± 0.051 > 0.599 ± 0.061
Sensitivity 0.571 > 0.535
Positive Predictive Value 0.719 > 0.516
Total TP 1717 > 1611
Total TN 350249 > 349515
Total FP 817 < 1767
Total FP CONTRA 70 < 194
Total FP INCONS 600 < 1316
Total FP COMP 147 < 257
Total FN 1292 < 1398
P-value 3.56938820447e-08

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Performance plots


  1. Comparison of performance of RNAshapes and MCFold. Positive Predictive Value (PPV) is plotted against sensitivity. Each dot represents a single test of each method. See tables below for raw data (individual counts for RNAshapes and MCFold).

  2. Average Matthews Correlation Coefficients (MCC) with 95% confidence intervals (CIs) were plotted for different RNA families, for which at least 3 members were present in the benchmarking dataset. 'n' denotes the number of MCCs used to calculate the average and CI. See tables below for raw data (individual counts for RNAshapes and MCFold).

  3. Comparison of average Matthews Correlation Coefficients (MCCs) for RNAshapes and MCFold. The whiskers correspond to 95% confidence intervals (CIs). 'n' denotes the number of MCCs used to calculate average MCCs and CIs. See tables below for raw data (individual counts for RNAshapes and MCFold).

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Performance of RNAshapes - scored higher in this pairwise comparison

1. Total counts & total scores for RNAshapes

Total Base Pair Counts
Total TP 1717
Total TN 350249
Total FP 817
Total FP CONTRA 70
Total FP INCONS 600
Total FP COMP 147
Total FN 1292
Total Scores
MCC 0.638
Average MCC ± 95% Confidence Intervals 0.681 ± 0.051
Sensitivity 0.571
Positive Predictive Value 0.719
Nr of predictions 131

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2. Individual counts for RNAshapes [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 395 0 0 0 0 0
2K63_A - 0.95 0.90 1.00 9 397 0 0 0 0 1
2K66_A - 0.95 0.90 1.00 9 222 0 0 0 0 1
2KD8_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
2KDQ_B 0.95 0.91 1.00 10 396 0 0 0 0 1
2KE6_A 0.97 0.95 1.00 18 1110 1 0 0 1 1
2KEZ_A - 0.89 0.80 1.00 8 268 0 0 0 0 2
2KFC_A - -0.02 0.00 0.00 0 623 7 0 7 0 20
2KGP_A - 1.00 1.00 1.00 9 291 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 367 0 0 0 0 0
2KPV_A - 0.96 0.92 1.00 12 549 0 0 0 0 1
2KU0_A - 0.83 0.69 1.00 11 692 0 0 0 0 5
2KUR_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KUU_A 0.92 0.86 1.00 18 1110 1 0 0 1 3
2KUV_A 0.93 0.86 1.00 19 1109 0 0 0 0 3
2KUW_A 0.95 0.90 1.00 19 1109 0 0 0 0 2
2KX8_A 0.91 0.83 1.00 15 846 0 0 0 0 3
2KXM_A - 0.83 0.70 1.00 7 344 0 0 0 0 3
2KZL_A - 0.86 0.74 1.00 14 1471 0 0 0 0 5
2L1F_A 0.87 0.83 0.91 20 2058 2 0 2 0 4
2L1F_B 0.96 0.92 1.00 23 2122 0 0 0 0 2
2L2K_A - 0.92 0.84 1.00 16 845 0 0 0 0 3
2L3C_B - 0.91 0.82 1.00 14 547 0 0 0 0 3
2L3E_A - 0.96 0.92 1.00 12 583 1 0 0 1 1
2L3J_B 0.92 0.85 1.00 29 2456 0 0 0 0 5
2L5Z_A - 0.86 0.75 1.00 9 316 0 0 0 0 3
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - 0.39 0.26 0.63 5 622 3 1 2 0 14
2LBS_A - 1.00 1.00 1.00 14 482 0 0 0 0 0
2LC8_A 0.61 0.55 0.69 11 1524 5 0 5 0 9
2LDL_A - 0.90 0.82 1.00 9 342 1 0 0 1 2
2LDT_A - 0.85 0.73 1.00 11 454 0 0 0 0 4
2LHP_A - 0.97 0.94 1.00 15 651 0 0 0 0 1
2LI4_A - 0.93 0.88 1.00 14 482 0 0 0 0 2
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 0.95 0.90 1.00 9 267 0 0 0 0 1
2LKR_A - 0.93 0.87 1.00 34 6071 1 0 0 1 5
2LQZ_A - 0.85 0.82 0.90 9 341 1 1 0 0 2
2LU0_A - 1.00 1.00 1.00 16 1160 0 0 0 0 0
2LWK_A - 0.83 0.77 0.91 10 485 1 0 1 0 3
2M58_A - 0.34 0.29 0.42 5 1641 7 1 6 0 12
2RP0_A - 0.64 0.42 1.00 5 346 0 0 0 0 7
2RPT_A - 1.00 1.00 1.00 7 183 0 0 0 0 0
2RRC_A - 0.70 0.50 1.00 5 226 0 0 0 0 5
2WRQ_Y 0.59 0.59 0.59 10 2833 12 4 3 5 7
2WW9_F - 0.59 0.60 0.60 6 290 4 0 4 0 4
2WW9_D - 0.16 0.12 0.25 3 1941 9 4 5 0 23
2WW9_E - 0.53 0.29 1.00 4 557 0 0 0 0 10
2WWQ_V 0.76 0.68 0.86 19 2904 5 0 3 2 9
2XKV_B 0.51 0.50 0.53 10 4541 22 0 9 13 10
2XQD_Y 0.64 0.56 0.75 15 2830 5 0 5 0 12
2XXA_G 0.93 0.86 1.00 36 5115 0 0 0 0 6
2Y8W_B - 1.00 1.00 1.00 6 184 1 0 0 1 0
2Y9C_V - 0.65 0.63 0.68 15 2256 8 1 6 1 9
2YIE_Z - -0.01 0.00 0.00 0 1525 15 3 12 0 12
2YIE_X - 0.25 0.25 0.27 3 1367 11 1 7 3 9
2ZY6_A - 0.58 0.58 0.58 7 691 5 0 5 0 5
2ZZN_D 0.69 0.63 0.77 17 2463 5 1 4 0 10
3A2K_C 0.42 0.39 0.46 11 2902 13 2 11 0 17
3A3A_A 0.76 0.65 0.89 24 3628 3 0 3 0 13
3AKZ_H 0.68 0.61 0.77 17 2679 6 1 4 1 11
3AM1_B - 0.64 0.60 0.70 21 3210 9 1 8 0 14
3AMU_B 0.70 0.59 0.84 16 2984 5 0 3 2 11
3DW4_A - -0.02 0.00 0.00 0 348 3 0 3 0 10
3GCA_A - 0.54 0.29 1.00 5 523 0 0 0 0 12
3GX2_A 0.76 0.63 0.93 25 4344 3 0 2 1 15
3HAY_E - 0.91 0.83 1.00 15 2263 4 0 0 4 3
3HJW_D - 0.94 0.89 1.00 16 1637 0 0 0 0 2
3IAB_R - 0.92 0.86 1.00 12 1023 0 0 0 0 2
3ID5_D - 0.44 0.33 0.60 3 491 5 0 2 3 6
3IVN_B 0.78 0.61 1.00 19 2327 0 0 0 0 12
3IWN_A 0.76 0.67 0.88 22 4253 3 0 3 0 11
3IZF_C 0.59 0.52 0.68 28 6862 13 1 12 0 26
3J0L_8 - 0.93 0.88 1.00 7 183 0 0 0 0 1
3J0L_2 - 0.38 0.36 0.40 12 6186 20 3 15 2 21
3J0L_g - -0.01 0.00 0.00 0 461 4 1 3 0 4
3J0L_7 - -0.01 0.00 0.00 0 1213 12 0 12 0 17
3J0L_h - 0.75 0.60 0.93 26 6077 4 0 2 2 17
3J0L_1 - 0.76 0.63 0.92 12 1212 2 0 1 1 7
3J0L_a - 0.23 0.19 0.30 3 1118 7 1 6 0 13
3J16_L 0.50 0.40 0.63 12 2756 7 0 7 0 18
3J20_0 0.43 0.40 0.48 12 2825 14 0 13 1 18
3J2L_3 0.59 0.51 0.69 27 7836 14 0 12 2 26
3J3D_C 0.60 0.54 0.68 15 2753 7 0 7 0 13
3J3E_8 0.17 0.15 0.19 5 7477 29 2 19 8 28
3J3E_7 0.48 0.41 0.58 22 7102 16 1 15 0 32
3J3F_7 0.68 0.58 0.81 29 7224 7 1 6 0 21
3J3F_8 0.30 0.31 0.30 11 12209 39 3 23 13 25
3JYV_7 -0.01 0.00 0.00 0 2830 20 0 20 0 32
3JYX_3 0.63 0.63 0.63 17 6301 21 1 9 11 10
3JYX_4 0.31 0.30 0.31 10 12214 35 5 17 13 23
3KTW_C - 0.39 0.35 0.45 15 4527 19 1 17 1 28
3LA5_A 0.76 0.59 1.00 20 2465 0 0 0 0 14
3NDB_M - 0.82 0.72 0.94 44 9133 4 0 3 1 17
3NKB_B - 0.61 0.54 0.70 14 1996 6 0 6 0 12
3NMU_E - 0.54 0.50 0.60 3 556 5 0 2 3 3
3O58_3 0.34 0.34 0.34 12 12368 37 2 21 14 23
3O58_2 0.60 0.61 0.59 23 7221 18 4 12 2 15
3OVB_D - 1.00 1.00 1.00 11 584 1 0 0 1 0
3OVS_D - 0.96 0.92 1.00 12 549 0 0 0 0 1
3P22_G - 0.96 0.92 1.00 11 730 0 0 0 0 1
3PDR_A 0.67 0.56 0.80 40 12830 12 0 10 2 32
3R4F_A - 0.96 0.92 1.00 23 2122 0 0 0 0 2
3R9X_C - 0.84 0.80 0.89 8 586 2 0 1 1 2
3RKF_A 0.76 0.59 1.00 20 2191 0 0 0 0 14
3SD1_A 0.64 0.52 0.79 22 3888 6 1 5 0 20
3SIU_F - 0.85 0.73 1.00 8 370 0 0 0 0 3
3SN2_B 0.96 0.92 1.00 11 395 0 0 0 0 1
3TRZ_Z - 0.91 0.83 1.00 5 205 1 0 0 1 1
3TS0_U - 1.00 1.00 1.00 6 247 1 0 0 1 0
3TS2_V - 1.00 1.00 1.00 5 271 0 0 0 0 0
3U4M_B - 0.38 0.32 0.46 12 3134 14 0 14 0 25
3VJR_D - 0.96 0.92 1.00 12 618 0 0 0 0 1
3ZEX_G - 0.52 0.45 0.61 33 16417 24 2 19 3 41
3ZEX_F - 0.00 0.00 0.00 0 2617 11 2 9 0 12
3ZEX_C 0.23 0.21 0.25 11 14152 41 1 32 8 41
3ZEX_H - 0.20 0.21 0.21 8 9006 31 5 26 0 30
3ZEX_D 0.76 0.63 0.91 31 6987 3 0 3 0 18
3ZND_W 0.20 0.22 0.19 5 2977 22 1 20 1 18
4A1C_2 0.14 0.15 0.14 5 11746 41 5 25 11 28
4A1C_3 0.68 0.57 0.82 31 7102 7 1 6 0 23
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4ATO_G - 0.32 0.30 0.38 3 520 5 1 4 0 7
4ENB_A 0.70 0.58 0.85 11 1262 2 1 1 0 8
4ENC_A 0.32 0.26 0.42 5 1314 7 1 6 0 14
4FNJ_A - 0.79 0.63 1.00 10 585 0 0 0 0 6
4FRG_B 0.32 0.28 0.38 9 3462 15 0 15 0 23
4FRN_A 0.52 0.44 0.62 16 5125 10 2 8 0 20
4HXH_A - 1.00 1.00 1.00 6 319 0 0 0 0 0
4JF2_A 0.78 0.61 1.00 19 2831 0 0 0 0 12
4JRC_A - 0.29 0.26 0.35 6 1523 11 0 11 0 17

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Performance of MCFold - scored lower in this pairwise comparison

1. Total counts & total scores for MCFold

Total Base Pair Counts
Total TP 1611
Total TN 349515
Total FP 1767
Total FP CONTRA 194
Total FP INCONS 1316
Total FP COMP 257
Total FN 1398
Total Scores
MCC 0.522
Average MCC ± 95% Confidence Intervals 0.599 ± 0.061
Sensitivity 0.535
Positive Predictive Value 0.516
Nr of predictions 131

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2. Individual counts for MCFold [ download as .csv ]

RNA Chain Rfam family MCC SENS PPV TP TN FP FP CONTRA FP INCONS FP COMP FN
2K5Z_A - 1.00 1.00 1.00 11 395 1 0 0 1 0
2K63_A - 0.90 0.90 0.90 9 396 4 0 1 3 1
2K66_A - 1.00 1.00 1.00 10 221 0 0 0 0 0
2KD8_A - 1.00 1.00 1.00 9 222 1 0 0 1 0
2KDQ_B 0.95 0.91 1.00 10 396 1 0 0 1 1
2KE6_A 1.00 1.00 1.00 19 1109 2 0 0 2 0
2KEZ_A - 0.90 0.90 0.90 9 266 1 0 1 0 1
2KFC_A - 0.28 0.25 0.36 5 616 9 2 7 0 15
2KGP_A - 1.00 1.00 1.00 9 291 1 0 0 1 0
2KMJ_A - 1.00 1.00 1.00 11 367 1 0 0 1 0
2KPV_A - 1.00 1.00 1.00 13 548 2 0 0 2 0
2KU0_A - 0.97 0.94 1.00 15 688 0 0 0 0 1
2KUR_A 1.00 1.00 1.00 21 1107 0 0 0 0 0
2KUU_A 0.98 0.95 1.00 20 1108 1 0 0 1 1
2KUV_A 0.98 0.95 1.00 21 1107 0 0 0 0 1
2KUW_A 0.95 0.95 0.95 20 1107 1 0 1 0 1
2KX8_A 0.91 0.89 0.94 16 844 2 0 1 1 2
2KXM_A - 1.00 1.00 1.00 10 341 1 0 0 1 0
2KZL_A - 0.80 0.84 0.76 16 1464 9 1 4 4 3
2L1F_A 0.72 0.75 0.69 18 2054 9 0 8 1 6
2L1F_B 0.73 0.76 0.70 19 2118 9 0 8 1 6
2L2K_A - 1.00 1.00 1.00 19 842 1 0 0 1 0
2L3C_B - 0.97 0.94 1.00 16 545 0 0 0 0 1
2L3E_A - 1.00 1.00 1.00 13 582 1 0 0 1 0
2L3J_B 1.00 1.00 1.00 34 2451 0 0 0 0 0
2L5Z_A - 0.96 0.92 1.00 11 314 0 0 0 0 1
2L94_A 0.97 0.95 1.00 19 971 0 0 0 0 1
2LA5_A - -0.03 0.00 0.00 0 615 15 0 15 0 19
2LBS_A - 1.00 1.00 1.00 14 482 1 0 0 1 0
2LC8_A 0.41 0.45 0.39 9 1517 16 0 14 2 11
2LDL_A - 0.95 0.91 1.00 10 341 2 0 0 2 1
2LDT_A - 0.75 0.73 0.79 11 451 3 0 3 0 4
2LHP_A - 1.00 1.00 1.00 16 650 1 0 0 1 0
2LI4_A - 0.97 0.94 1.00 15 481 0 0 0 0 1
2LJJ_A - 1.00 1.00 1.00 8 343 2 0 0 2 0
2LK3_A - 1.00 1.00 1.00 10 266 0 0 0 0 0
2LKR_A - 0.93 0.92 0.95 36 6067 14 0 2 12 3
2LQZ_A - 0.91 0.91 0.91 10 340 1 1 0 0 1
2LU0_A - 1.00 1.00 1.00 16 1160 5 0 0 5 0
2LWK_A - 0.92 0.92 0.92 12 483 2 0 1 1 1
2M58_A - 0.20 0.24 0.18 4 1631 19 2 16 1 13
2RP0_A - 0.44 0.42 0.50 5 341 5 1 4 0 7
2RPT_A - 1.00 1.00 1.00 7 183 2 0 0 2 0
2RRC_A - 0.95 0.90 1.00 9 222 0 0 0 0 1
2WRQ_Y 0.27 0.35 0.22 6 2823 25 8 13 4 11
2WW9_F - 0.59 0.60 0.60 6 290 4 0 4 0 4
2WW9_D - -0.01 0.00 0.00 0 1925 28 5 23 0 26
2WW9_E - -0.02 0.00 0.00 0 547 14 0 14 0 14
2WWQ_V 0.15 0.18 0.15 5 2892 30 4 25 1 23
2XKV_B 0.20 0.25 0.17 5 4530 37 2 23 12 15
2XQD_Y 0.34 0.37 0.32 10 2819 22 1 20 1 17
2XXA_G 0.23 0.24 0.24 10 5110 32 1 30 1 32
2Y8W_B - 1.00 1.00 1.00 6 184 2 0 0 2 0
2Y9C_V - 0.62 0.67 0.59 16 2251 13 2 9 2 8
2YIE_Z - 0.36 0.50 0.27 6 1518 18 4 12 2 6
2YIE_X - 0.06 0.08 0.06 1 1361 21 1 15 5 11
2ZY6_A - 0.51 0.58 0.47 7 688 8 3 5 0 5
2ZZN_D 0.61 0.63 0.61 17 2457 13 1 10 2 10
3A2K_C 0.44 0.46 0.42 13 2895 18 2 16 0 15
3A3A_A 0.89 0.86 0.91 32 3620 3 1 2 0 5
3AKZ_H 0.40 0.43 0.39 12 2670 19 3 16 0 16
3AM1_B - 0.93 0.89 0.97 31 3208 2 0 1 1 4
3AMU_B 0.44 0.48 0.42 13 2972 18 2 16 0 14
3DW4_A - 0.90 0.90 0.90 9 341 3 0 1 2 1
3GCA_A - 0.04 0.06 0.07 1 514 13 1 12 0 16
3GX2_A 0.47 0.48 0.48 19 4331 22 0 21 1 21
3HAY_E - 1.00 1.00 1.00 18 2260 11 0 0 11 0
3HJW_D - 0.94 0.94 0.94 17 1635 8 0 1 7 1
3IAB_R - 0.84 0.93 0.76 13 1018 8 2 2 4 1
3ID5_D - 0.34 0.33 0.38 3 488 9 0 5 4 6
3IVN_B 0.39 0.39 0.40 12 2316 18 0 18 0 19
3IWN_A 0.19 0.21 0.18 7 4238 33 8 25 0 26
3IZF_C 0.71 0.69 0.74 37 6853 14 0 13 1 17
3J0L_8 - 1.00 1.00 1.00 8 182 0 0 0 0 0
3J0L_2 - 0.21 0.24 0.18 8 6172 39 7 29 3 25
3J0L_g - 0.13 0.25 0.08 1 452 12 8 4 0 3
3J0L_7 - -0.01 0.00 0.00 0 1206 19 4 15 0 17
3J0L_h - 0.91 0.91 0.91 39 6062 13 0 4 9 4
3J0L_1 - 0.79 0.79 0.79 15 1206 6 0 4 2 4
3J0L_a - 0.16 0.19 0.17 3 1110 15 1 14 0 13
3J16_L 0.45 0.47 0.44 14 2743 19 1 17 1 16
3J20_0 0.59 0.57 0.63 17 2823 12 1 9 2 13
3J2L_3 0.58 0.57 0.60 30 7825 23 2 18 3 23
3J3D_C 0.42 0.43 0.43 12 2747 18 2 14 2 16
3J3E_8 0.12 0.12 0.12 4 7470 46 3 26 17 29
3J3E_7 0.40 0.39 0.42 21 7090 29 3 26 0 33
3J3F_7 0.74 0.74 0.74 37 7210 17 2 11 4 13
3J3F_8 0.13 0.17 0.11 6 12191 61 9 40 12 30
3JYV_7 0.21 0.22 0.21 7 2817 26 0 26 0 25
3JYX_3 0.41 0.52 0.33 14 6285 34 12 17 5 13
3JYX_4 0.20 0.24 0.17 8 12199 43 16 23 4 25
3KTW_C - 0.40 0.40 0.43 17 4520 24 4 19 1 26
3LA5_A 0.32 0.32 0.34 11 2453 21 1 20 0 23
3NDB_M - 0.23 0.23 0.24 14 9121 46 1 44 1 47
3NKB_B - 0.56 0.58 0.56 15 1989 13 0 12 1 11
3NMU_E - 0.28 0.33 0.25 2 553 12 0 6 6 4
3O58_3 0.22 0.26 0.19 9 12355 45 9 30 6 26
3O58_2 0.20 0.24 0.17 9 7208 44 5 38 1 29
3OVB_D - 1.00 1.00 1.00 11 584 2 0 0 2 0
3OVS_D - 1.00 1.00 1.00 13 548 0 0 0 0 0
3P22_G - 1.00 1.00 1.00 12 729 5 0 0 5 0
3PDR_A 0.66 0.61 0.72 44 12819 19 0 17 2 28
3R4F_A - 0.92 0.92 0.92 23 2120 5 0 2 3 2
3R9X_C - 0.69 0.70 0.70 7 585 7 0 3 4 3
3RKF_A 0.70 0.65 0.76 22 2182 7 1 6 0 12
3SD1_A 0.33 0.33 0.35 14 3876 26 0 26 0 28
3SIU_F - 0.42 0.45 0.42 5 366 7 1 6 0 6
3SN2_B 0.55 0.58 0.54 7 393 6 0 6 0 5
3TRZ_Z - -0.04 0.00 0.00 0 201 9 0 9 0 6
3TS0_U - 1.00 1.00 1.00 6 247 2 0 0 2 0
3TS2_V - -0.02 0.00 0.00 0 268 9 0 8 1 5
3U4M_B - 0.59 0.59 0.59 22 3123 15 0 15 0 15
3VJR_D - 1.00 1.00 1.00 13 617 1 0 0 1 0
3ZEX_G - 0.00 0.00 0.00 0 16456 15 1 14 0 74
3ZEX_F - -0.01 0.00 0.00 0 2610 29 4 14 11 12
3ZEX_C 0.24 0.21 0.28 11 14156 29 3 26 0 41
3ZEX_H - 0.14 0.18 0.12 7 8987 52 14 37 1 31
3ZEX_D 0.17 0.18 0.18 9 6970 42 4 38 0 40
3ZND_W 0.19 0.22 0.18 5 2975 26 1 22 3 18
4A1C_2 0.13 0.15 0.11 5 11735 56 10 31 15 28
4A1C_3 0.68 0.67 0.71 36 7089 17 1 14 2 18
4A4U_A - 1.00 1.00 1.00 9 222 0 0 0 0 0
4ATO_G - 0.30 0.30 0.33 3 519 8 0 6 2 7
4ENB_A 0.61 0.63 0.60 12 1255 8 2 6 0 7
4ENC_A 0.28 0.32 0.27 6 1304 17 2 14 1 13
4FNJ_A - 0.83 0.81 0.87 13 580 3 0 2 1 3
4FRG_B 0.32 0.34 0.31 11 3450 25 0 25 0 21
4FRN_A 0.12 0.14 0.12 5 5110 38 1 35 2 31
4HXH_A - 1.00 1.00 1.00 6 319 4 0 0 4 0
4JF2_A 0.66 0.68 0.66 21 2818 11 0 11 0 10
4JRC_A - 0.20 0.22 0.21 5 1516 19 0 19 0 18

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Matthews Correlation Coeffient, Sensitivity and Positive Predictive Value have been calculated based on the paper by Gardener & Giegerich, 2004.