Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily
Overview of Jesenská A et al.
| Authors | Jesenská A Monincová M Koudeláková T Hasan K Chaloupková R Prokop Z Geerlof A Damborsky J |
|---|---|
| Affiliation | Institute of Experimental Biology and National Center for Biomolecular Research Masaryk University Brno Czech Republic. |
| Journal | Appl Environ Microbiol |
| Year | 2009 |
Abstract
This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.