Modomics - A Database of RNA Modifications

ID Card:

Full name: rRNA adenine N-6-methyltransferase
GI: 127199
COG: COG0030
UniProt: P10738
Alpha Fold Predicted Structure: AF-P10738-F1
Enzyme type: methyltransferase
Position of modification - modification: l:2058(2058) - m6A



Protein sequence:

MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKSNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK

Comments:

ErmBC methylates the exocyclic amine of A2058 in the Peptidyl Center (between helix 73 and 74) of 23S rRNA. It is responsible for the development of bacterial resistance to lincosamide/ Streptogramin-type of antibiotics. This enzyme arose in E. coli by lateral gene transfer from a gram-positive bacteria Brisson-Noël et al. 1988 ).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:m6A RNA rRNA 2058 LSU - 23S exocyclic amine of A2058 in Peptidyl Center between helix 73 and 74 Prokaryotic Cytosol 2832378   

Alpha Fold Predicted Structure:






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Protein sequence:

M N K N I K Y S Q N F L T S E K V L N Q I I K Q L N L K E T D T V Y E I G T G K G H L T T K L A K I S K Q V T S I E L D S H L F N L S S E K L K S N T R V T L I H Q D I L Q F Q F P N K Q R Y K I V G N I P Y H L S T Q I I K K V V F E S H A S D I Y L I V E E G F Y K R T L D I H R T L G L L L H T Q V S I Q Q L L K L P A E C F H P K P R V N S V L I K L T R H T T D V P D K Y W K L Y T Y F V S K W V N R E Y R Q L F T K N Q F H Q A M K H A K V N N L S T V T Y E Q V L S I F N S Y L L F N G R K

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P10738-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P10738-F1.cif  
DSSP Secondary Structures   P10738.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Evidence for natural gene transfer from gram-positive cocci to Escherichia coli. Brisson-Noel A, Arthur M, Courvalin P J Bacteriol [details] 2832378 -

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