ID Card:
Full name:
tRNA A64-2′-O-ribosylphosphate transferase
GI:
1350603
Orf:
YMR283C, YM8021.09C
COG:
COG2365
UniProt:
P23796
Alpha Fold Predicted Structure:
AF-P23796-F1
Enzyme type:
phosphotransferase
Position of modification - modification:
t :64 - Ar(p)
Protein sequence:
MVSRLSKRYRSRVQFSISVMDENIYLSLSQINKDIRKENKSVRNRLQSILLDNKFLQDRVIPIFPHYPLIPNERCGLWYCNPSSFKQTSYFKSTDGHVNQWDFSTRRLNFHLLETIRDNKGIIIVDSTRRGKKIPDALSKTVPIWCAVLNTLMLQETEKNVAIDKVLYLPPETVPKSEYDMIKRKIPELVAKLQKLNIIDSKKLNELFMGKLLRPIWVHPGSSLLDHSVDYFTGEVQEYEAWETPEDQNIIPIILCTVSYQAQDGMDKRYGFTYVQGAADDHELWSFGLDSNMFWAHIEYLGDASYSDDQLHDYIMDLAAAKLRNQCYIQDKGSLDEVFGNIDKITNEISLGKVSSGLTINKNLKQKLKSEYGKVIIFSNSVTVAEDTDDEEESGTDPFISIYKLQSGDKKSSKALRSTFPRIHGEIQSLFTNRDEKIKPMLICCNTGTDMSIGVILSILCTKYTEEWMLTSELPDISKLIVRKHLTKLISHLKGRNVNPSRATLNSVNSFLM
Comments:
Rit1 encodes a phospho-ribosyl transferase that exclusively modifies the initiator tRNA (tRNAMet(i)) by the addition of a 2'-O-ribosyl phosphate group to Adenosine 64. 5'-phosphoribosyl-1'-pyrophosphate is used as the modification donor. As a result, tRNAMet(i) is prevented from participating in the elongation steps of protein synthesis.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
A:Ar(p)
RNA
tRNA
64
TΨC-stem loop
Cytoplasm
Alpha Fold Predicted Structure:
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Protein sequence:
M
V
S
R
L
S
K
R
Y
R
S
R
V
Q
F
S
I
S
V
M
D
E
N
I
Y
L
S
L
S
Q
I
N
K
D
I
R
K
E
N
K
S
V
R
N
R
L
Q
S
I
L
L
D
N
K
F
L
Q
D
R
V
I
P
I
F
P
H
Y
P
L
I
P
N
E
R
C
G
L
W
Y
C
N
P
S
S
F
K
Q
T
S
Y
F
K
S
T
D
G
H
V
N
Q
W
D
F
S
T
R
R
L
N
F
H
L
L
E
T
I
R
D
N
K
G
I
I
I
V
D
S
T
R
R
G
K
K
I
P
D
A
L
S
K
T
V
P
I
W
C
A
V
L
N
T
L
M
L
Q
E
T
E
K
N
V
A
I
D
K
V
L
Y
L
P
P
E
T
V
P
K
S
E
Y
D
M
I
K
R
K
I
P
E
L
V
A
K
L
Q
K
L
N
I
I
D
S
K
K
L
N
E
L
F
M
G
K
L
L
R
P
I
W
V
H
P
G
S
S
L
L
D
H
S
V
D
Y
F
T
G
E
V
Q
E
Y
E
A
W
E
T
P
E
D
Q
N
I
I
P
I
I
L
C
T
V
S
Y
Q
A
Q
D
G
M
D
K
R
Y
G
F
T
Y
V
Q
G
A
A
D
D
H
E
L
W
S
F
G
L
D
S
N
M
F
W
A
H
I
E
Y
L
G
D
A
S
Y
S
D
D
Q
L
H
D
Y
I
M
D
L
A
A
A
K
L
R
N
Q
C
Y
I
Q
D
K
G
S
L
D
E
V
F
G
N
I
D
K
I
T
N
E
I
S
L
G
K
V
S
S
G
L
T
I
N
K
N
L
K
Q
K
L
K
S
E
Y
G
K
V
I
I
F
S
N
S
V
T
V
A
E
D
T
D
D
E
E
E
S
G
T
D
P
F
I
S
I
Y
K
L
Q
S
G
D
K
K
S
S
K
A
L
R
S
T
F
P
R
I
H
G
E
I
Q
S
L
F
T
N
R
D
E
K
I
K
P
M
L
I
C
C
N
T
G
T
D
M
S
I
G
V
I
L
S
I
L
C
T
K
Y
T
E
E
W
M
L
T
S
E
L
P
D
I
S
K
L
I
V
R
K
H
L
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K
L
I
S
H
L
K
G
R
N
V
N
P
S
R
A
T
L
N
S
V
N
S
F
L
M
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination.
Astrom SU, Bystrom AS
Cell
[details]
7954819
-