Modomics - A Database of RNA Modifications

Full name: tRNA A64-2′-O-ribosylphosphate transferase
Synonym:
GI: 1350603
Orf: YMR283C, YM8021.09C
COG: COG2365
UniProt: P23796
Structures: | |
Complex:
Enzyme type: phosphotransferase
Position of modification - modification: t:64 - Ar(p)

Comments:

Rit1 encodes a phospho-ribosyl transferase that exclusively modifies the initiator tRNA (tRNAMet(i)) by the addition of a 2'-O-ribosyl phosphate group to Adenosine 64. 5'-phosphoribosyl-1'-pyrophosphate is used as the modification donor. As a result, tRNAMet(i) is prevented from participating in the elongation steps of protein synthesis.

Protein sequence:

MVSRLSKRYRSRVQFSISVMDENIYLSLSQINKDIRKENKSVRNRLQSILLDNKFLQDRVIPIFPHYPLIPNERCGLWYCNPSSFKQTSYFKSTDGHVNQ
WDFSTRRLNFHLLETIRDNKGIIIVDSTRRGKKIPDALSKTVPIWCAVLNTLMLQETEKNVAIDKVLYLPPETVPKSEYDMIKRKIPELVAKLQKLNIID
SKKLNELFMGKLLRPIWVHPGSSLLDHSVDYFTGEVQEYEAWETPEDQNIIPIILCTVSYQAQDGMDKRYGFTYVQGAADDHELWSFGLDSNMFWAHIEY
LGDASYSDDQLHDYIMDLAAAKLRNQCYIQDKGSLDEVFGNIDKITNEISLGKVSSGLTINKNLKQKLKSEYGKVIIFSNSVTVAEDTDDEEESGTDPFI
SIYKLQSGDKKSSKALRSTFPRIHGEIQSLFTNRDEKIKPMLICCNTGTDMSIGVILSILCTKYTEEWMLTSELPDISKLIVRKHLTKLISHLKGRNVNP
SRATLNSVNSFLM

Enzymatic activities:

Reaction Substrate Type Position
A:Ar(p) tRNA (t)   64

Publications:

Title Authors Journal Details PubMed Id DOI
Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination. Astrom SU, Bystrom AS Cell [details] 7954819 -

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