| Full name: |
Leucine carboxyl methyltransferase 2 |
| Synonym: |
PPM2, LCMT2 |
| GI: |
1420038 |
| Orf: |
YOL141W |
| COG: |
|
| UniProt: |
Q08282 |
| Structures: |
|
2ZW9 |
2ZWA |
2ZZK |
|
| Complex: |
|
| Enzyme type: |
methyltransferase, carboxymethyltransferase |
| Position of modification - modification: |
t:37 - yW
|
Comments:
TYW4 is a Ado-Met-dependent carboxymethyltransferase. It catalyzes two reactions: methylation of the α-carboxy group of yW-72 to form yW-58 and methoxycarbonylation of α-amino group of yW-58 to complete yW. It is proposed that these two reactions are performed with a single catalytic site. The methoxycarbonylation reaction may proceed through carbamate formation with CO2 and methylation with SAM, which results in CO2 fixation
Protein sequence:
MKNLTTIKQTNKNVKQERRKKYADLAIQGTNNSSIASKRSVELLYLPKLSSANNFQMDKNNKLLEYFKFFVPKKIKRSPCINRGYWLRLFAIRSRLNSII
EQTPQDKKIVVVNLGCGYDPLPFQLLDTNNIQSQQYHDRVSFIDIDYSDLLKIKIELIKTIPELSKIIGLSEDKDYVDDSNVDFLTTPKYLARPCDLNDS
KMFSTLLNECQLYDPNVVKVFVAEVSLAYMKPERSDSIIEATSKMENSHFIILEQLIPKGPFEPFSKQMLAHFKRNDSPLQSVLKYNTIESQVQRFNKLG
FAYVNVGDMFQLWESADEATKKELLKVEPFDELEEFHLFCHHYVLCHATNYKEFAFTQGFLFDRSISEINLTVDEDYQLLECECPINRKFGDVDVAGNDV
FYMGGSNPYRVNEILQMSIHYDKIDMKNIEVSSSEVPVARMCHTFTTISRNNQLLLIGGRKAPHQGLSDNWIFDMKTREWSMIKSLSHTRFRHSACSLPD
GNVLILGGVTEGPAMLLYNVTEEIFKDVTPKDEFFQNSLVSAGLEFDPVSKQGIILGGGFMDQTTVSDKAIIFKYDAENATEPITVIKKLQHPLFQRYGS
QIKYITPRKLLIVGGTSPSGLFDRTNSIISLDPLSETLTSIPISRRIWEDHSLMLAGFSLVSTSMGTIHIIGGGATCYGFGSVTNVGLKLIAIAK
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
yW-58:yW
|
tRNA (t) |
Phe/#AA/eukaryotic cytosol |
37 |
|
yW-72:yW-58
|
tRNA (t) |
Phe/#AA/eukaryotic cytosol |
37 |
|
yW-72:yW-58
|
tRNA (t) |
Phe/#AA/eukaryotic cytosol |
37 |
|
yW-58:yW
|
tRNA (t) |
Phe/#AA/eukaryotic cytosol |
37 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. |
Noma A, Kirino Y, Ikeuchi Y, Suzuki T |
EMBO J |
[details]
|
16642040
|
-
|
| Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4. |
Suzuki Y, Noma A, Suzuki T, Ishitani R, Nureki O |
Nucleic Acids Res |
[details]
|
19287006
|
-
|
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