Full name: Leucine carboxyl methyltransferase 2
Synonym: PPM2, LCMT2
GI: 1420038
Orf: YOL141W
COG:
UniProt: Q08282
Structures: | 2ZW9 | 2ZWA | 2ZZK |
Complex:
Enzyme type: methyltransferase, carboxymethyltransferase
Position of modification - modification: t:37 - yW

Comments:

TYW4 is a Ado-Met-dependent carboxymethyltransferase. It catalyzes two reactions: methylation of the α-carboxy group of yW-72 to form yW-58 and methoxycarbonylation of α-amino group of yW-58 to complete yW. It is proposed that these two reactions are performed with a single catalytic site. The methoxycarbonylation reaction may proceed through carbamate formation with CO2 and methylation with SAM, which results in CO2 fixation

Protein sequence:

MKNLTTIKQTNKNVKQERRKKYADLAIQGTNNSSIASKRSVELLYLPKLSSANNFQMDKNNKLLEYFKFF VPKKIKRSPCINRGYWLRLFAIRSRLNSIIEQTPQDKKIVVVNLGCGYDPLPFQLLDTNNIQSQQYHDRV SFIDIDYSDLLKIKIELIKTIPELSKIIGLSEDKDYVDDSNVDFLTTPKYLARPCDLNDSKMFSTLLNEC QLYDPNVVKVFVAEVSLAYMKPERSDSIIEATSKMENSHFIILEQLIPKGPFEPFSKQMLAHFKRNDSPL QSVLKYNTIESQVQRFNKLGFAYVNVGDMFQLWESADEATKKELLKVEPFDELEEFHLFCHHYVLCHATN YKEFAFTQGFLFDRSISEINLTVDEDYQLLECECPINRKFGDVDVAGNDVFYMGGSNPYRVNEILQMSIH YDKIDMKNIEVSSSEVPVARMCHTFTTISRNNQLLLIGGRKAPHQGLSDNWIFDMKTREWSMIKSLSHTR FRHSACSLPDGNVLILGGVTEGPAMLLYNVTEEIFKDVTPKDEFFQNSLVSAGLEFDPVSKQGIILGGGF MDQTTVSDKAIIFKYDAENATEPITVIKKLQHPLFQRYGSQIKYITPRKLLIVGGTSPSGLFDRTNSIIS LDPLSETLTSIPISRRIWEDHSLMLAGFSLVSTSMGTIHIIGGGATCYGFGSVTNVGLKLIAIAK

Enzymatic activities:

Reaction Substrate Type Position
yW-58:yW tRNA (t) Phe/#AA/cytosolic 37
yW-72:yW-58 tRNA (t) Phe/#AA/cytosolic 37
yW-72:yW-58 tRNA (t) Phe/#AA/cytosolic 37
yW-58:yW tRNA (t) Phe/#AA/cytosolic 37

Publications:

Title Authors Journal Details PubMed Id DOI
Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. Noma A, Kirino Y, Ikeuchi Y, Suzuki T EMBO J [details] 16642040 -
Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4. Suzuki Y, Noma A, Suzuki T, Ishitani R, Nureki O Nucleic Acids Res [details] 19287006 -

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