ID Card:
| Full name: |
tRNA wybutosine-synthesizing protein 1 |
| GI: |
2501612
|
| Orf: |
YPL207W |
| COG: |
COG0731 |
| UniProt: |
Q08960 |
| Alpha Fold Predicted Structure: |
AF-Q08960-F1 |
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
t:37 - yW
|
Protein sequence:
MDGFRVAGALVVGALTAAYLYFGGRFSIALVIIVGYGIYCNEASGGSQDSQEKLDLNKQQKKPCCSDKKIADGGKKTGGCCSDKKNGGGKGGGCCSSKGGKKGGCCSSKGGKKGGCCSSKKNIGDNENTATEVEKAVNYPVTVDFTEVFRKPTKKRSSTPKVFSKNSSSNSRVGKKLSVSKKIGPDGLIKSALTISNETLLSSQIYVLYSSLQGAASKAAKSVYDKLKELDELTNEPKLLNLDDLSDFDDYFINVPVENALYVLVLPSYDIDCPLDYFLQTLEENANDFRVDSFPLRKLVGYTVLGLGDSESWPEKFCYQAKRADHWISRLGGRRIFPLGKVCMKTGGSAKIDEWTSLLAETLKDDEPIIYEYDENADSEEDEEEGNGSDELGDVEDIGGKGSNGKFSGADEIKQMVAKDSPTYKNLTKQGYKVIGSHSGVKICRWTKNELRGKGSCYKKSLFNIASSRCMELTPSLACSSKCVFCWRHGTNPVSKNWRWEVDEPEYILENALKGHYSMIKQMRGVPGVIAERFAKAFEVRHCALSLVGEPILYPHINKFIQLLHQKGITSFLVCNAQHPEALRNIVKVTQLYVSIDAPTKTELKKVDRPLYKDFWERMVECLEILKTVQNHQRTVFRLTLVKGFNMGDVSAYADLVQRGLPGFIEVKGATFSGSSDGNGNPLTMQNIPFYEECVKFVKAFTTELQRRGLHYDLAAEHAHSNCLLIADTKFKINGEWHTHIDFDKFFVLLNSGKDFTYMDYLEKTPEWALFGNGGFAPGNTRVYRKDKKKQNKENQETTTRETPLPPIPA
Comments:
An Fe-S cluster protein, *may* catalyze tricyclic formation of yW using Ado-Met and FMN as cofactors. Radical SAM enzyme.
| Reaction |
Substrate |
SubstrateType |
Position |
(Anti)Codon |
Modified (Anti)Codon |
Amino Acid Change |
Transcript Name |
Transcript Region |
Cellular Localization |
References |
|
m1G:imG-14
|
RNA |
tRNA |
37 |
#AA |
#AA |
|
tRNAPhe#AA |
|
Endoplasmic reticulum |
|
Alpha Fold Predicted Structure:
Clear Selection and Reset Camera
Protein sequence:
M
D
G
F
R
V
A
G
A
L
V
V
G
A
L
T
A
A
Y
L
Y
F
G
G
R
F
S
I
A
L
V
I
I
V
G
Y
G
I
Y
C
N
E
A
S
G
G
S
Q
D
S
Q
E
K
L
D
L
N
K
Q
Q
K
K
P
C
C
S
D
K
K
I
A
D
G
G
K
K
T
G
G
C
C
S
D
K
K
N
G
G
G
K
G
G
G
C
C
S
S
K
G
G
K
K
G
G
C
C
S
S
K
G
G
K
K
G
G
C
C
S
S
K
K
N
I
G
D
N
E
N
T
A
T
E
V
E
K
A
V
N
Y
P
V
T
V
D
F
T
E
V
F
R
K
P
T
K
K
R
S
S
T
P
K
V
F
S
K
N
S
S
S
N
S
R
V
G
K
K
L
S
V
S
K
K
I
G
P
D
G
L
I
K
S
A
L
T
I
S
N
E
T
L
L
S
S
Q
I
Y
V
L
Y
S
S
L
Q
G
A
A
S
K
A
A
K
S
V
Y
D
K
L
K
E
L
D
E
L
T
N
E
P
K
L
L
N
L
D
D
L
S
D
F
D
D
Y
F
I
N
V
P
V
E
N
A
L
Y
V
L
V
L
P
S
Y
D
I
D
C
P
L
D
Y
F
L
Q
T
L
E
E
N
A
N
D
F
R
V
D
S
F
P
L
R
K
L
V
G
Y
T
V
L
G
L
G
D
S
E
S
W
P
E
K
F
C
Y
Q
A
K
R
A
D
H
W
I
S
R
L
G
G
R
R
I
F
P
L
G
K
V
C
M
K
T
G
G
S
A
K
I
D
E
W
T
S
L
L
A
E
T
L
K
D
D
E
P
I
I
Y
E
Y
D
E
N
A
D
S
E
E
D
E
E
E
G
N
G
S
D
E
L
G
D
V
E
D
I
G
G
K
G
S
N
G
K
F
S
G
A
D
E
I
K
Q
M
V
A
K
D
S
P
T
Y
K
N
L
T
K
Q
G
Y
K
V
I
G
S
H
S
G
V
K
I
C
R
W
T
K
N
E
L
R
G
K
G
S
C
Y
K
K
S
L
F
N
I
A
S
S
R
C
M
E
L
T
P
S
L
A
C
S
S
K
C
V
F
C
W
R
H
G
T
N
P
V
S
K
N
W
R
W
E
V
D
E
P
E
Y
I
L
E
N
A
L
K
G
H
Y
S
M
I
K
Q
M
R
G
V
P
G
V
I
A
E
R
F
A
K
A
F
E
V
R
H
C
A
L
S
L
V
G
E
P
I
L
Y
P
H
I
N
K
F
I
Q
L
L
H
Q
K
G
I
T
S
F
L
V
C
N
A
Q
H
P
E
A
L
R
N
I
V
K
V
T
Q
L
Y
V
S
I
D
A
P
T
K
T
E
L
K
K
V
D
R
P
L
Y
K
D
F
W
E
R
M
V
E
C
L
E
I
L
K
T
V
Q
N
H
Q
R
T
V
F
R
L
T
L
V
K
G
F
N
M
G
D
V
S
A
Y
A
D
L
V
Q
R
G
L
P
G
F
I
E
V
K
G
A
T
F
S
G
S
S
D
G
N
G
N
P
L
T
M
Q
N
I
P
F
Y
E
E
C
V
K
F
V
K
A
F
T
T
E
L
Q
R
R
G
L
H
Y
D
L
A
A
E
H
A
H
S
N
C
L
L
I
A
D
T
K
F
K
I
N
G
E
W
H
T
H
I
D
F
D
K
F
F
V
L
L
N
S
G
K
D
F
T
Y
M
D
Y
L
E
K
T
P
E
W
A
L
F
G
N
G
G
F
A
P
G
N
T
R
V
Y
R
K
D
K
K
K
Q
N
K
E
N
Q
E
T
T
T
R
E
T
P
L
P
P
I
P
A
Secondary Structure Alphabet
- G: 3-turn helix (310helix)
- H: α-helix
- I: 𝝅-helix (5 - turn helix)
- T: Hydrogen Bonded Turn
- B: β-sheet
- S: Bend
- C: Coil (residues not present in any of the above conformations)
- N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. |
Noma A, Kirino Y, Ikeuchi Y, Suzuki T |
EMBO J |
[details]
|
16642040
|
-
|
| Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs. |
Waas WF, de Crecy-Lagard V, Schimmel P |
J Biol Chem |
[details]
|
16162496
|
-
|
| Radical mediated ring formation in the biosynthesis of the hypermodified tRNA base wybutosine. |
Young AP, Bandarian V... |
Curr Opin Chem Biol |
[details]
|
23856057
|
-
|
Links: