ID Card:
| Full name: |
tRNA wybutosine-synthesizing protein 2 |
| Synonym: |
Trm12, YMA5 |
| GI: |
2497109
|
| Orf: |
YML005W |
| COG: |
COG2520 |
| UniProt: |
Q04235 |
| Alpha Fold Predicted Structure: |
AF-Q04235-F1 |
| Enzyme type: |
Alpha-amino-alpha-carboxypropyltransferase |
| Position of modification - modification: |
t:37 - yW
|
Protein sequence:
MSDETMPVEFLVSDKRLLKTIKVKLETNGLFVTPIYSDNDNKVIKSSIEDLNHPLAVEINNIAGVKARFHESGNLERSEGHLKHQSNSITEFTKSFLKDHGLANDKIFLSHLLDHLPLKYTIYPPVVLFNNSTVRSFNHPIWQKAFQLKLFDPNEYYRELLCFLSPGKPSKGTSLHPNNRLLTHLAINNPITEADVLRRPFNIQPLYGKLIDDSILDDNDNTLWENPSQEQLNSSIWCKVIQNGVTQIWSPVFTMFSRGNIKEKKRVLTTFPDICNNDVVDLYAGIGYFTFSYLTKGARTLFAFELNPWSVEGLKRGLKANGFNKSGNCHVFQESNEMCVQRLTEFLSQNPGFRLRIRHINLGLLPSSKQGWPLAIKLIYLQGASLEKVTMHIHENVHIDAIEDGSFEKNVIVELDAINESIALIRNRGIKLQFVRSKLERIKTFAPDIWHVCVDVDVIVST
Comments:
TYW2 transfers α-amino-α-carboxypropyl group from Ado-Met to the side chain at the C-7 position of the yW-187 (imG-14) to produce yW-86.
| Reaction |
Substrate |
SubstrateType |
Position |
(Anti)Codon |
Modified (Anti)Codon |
Amino Acid Change |
Transcript Name |
Transcript Region |
Cellular Localization |
References |
|
imG-14:yW-86
|
RNA |
tRNA |
37 |
#AA |
#AA |
|
tRNAPhe#AA |
anticodon-loop |
Cytoplasm |
16642040
16005430
|
Alpha Fold Predicted Structure:
Clear Selection and Reset Camera
Protein sequence:
M
S
D
E
T
M
P
V
E
F
L
V
S
D
K
R
L
L
K
T
I
K
V
K
L
E
T
N
G
L
F
V
T
P
I
Y
S
D
N
D
N
K
V
I
K
S
S
I
E
D
L
N
H
P
L
A
V
E
I
N
N
I
A
G
V
K
A
R
F
H
E
S
G
N
L
E
R
S
E
G
H
L
K
H
Q
S
N
S
I
T
E
F
T
K
S
F
L
K
D
H
G
L
A
N
D
K
I
F
L
S
H
L
L
D
H
L
P
L
K
Y
T
I
Y
P
P
V
V
L
F
N
N
S
T
V
R
S
F
N
H
P
I
W
Q
K
A
F
Q
L
K
L
F
D
P
N
E
Y
Y
R
E
L
L
C
F
L
S
P
G
K
P
S
K
G
T
S
L
H
P
N
N
R
L
L
T
H
L
A
I
N
N
P
I
T
E
A
D
V
L
R
R
P
F
N
I
Q
P
L
Y
G
K
L
I
D
D
S
I
L
D
D
N
D
N
T
L
W
E
N
P
S
Q
E
Q
L
N
S
S
I
W
C
K
V
I
Q
N
G
V
T
Q
I
W
S
P
V
F
T
M
F
S
R
G
N
I
K
E
K
K
R
V
L
T
T
F
P
D
I
C
N
N
D
V
V
D
L
Y
A
G
I
G
Y
F
T
F
S
Y
L
T
K
G
A
R
T
L
F
A
F
E
L
N
P
W
S
V
E
G
L
K
R
G
L
K
A
N
G
F
N
K
S
G
N
C
H
V
F
Q
E
S
N
E
M
C
V
Q
R
L
T
E
F
L
S
Q
N
P
G
F
R
L
R
I
R
H
I
N
L
G
L
L
P
S
S
K
Q
G
W
P
L
A
I
K
L
I
Y
L
Q
G
A
S
L
E
K
V
T
M
H
I
H
E
N
V
H
I
D
A
I
E
D
G
S
F
E
K
N
V
I
V
E
L
D
A
I
N
E
S
I
A
L
I
R
N
R
G
I
K
L
Q
F
V
R
S
K
L
E
R
I
K
T
F
A
P
D
I
W
H
V
C
V
D
V
D
V
I
V
S
T
Secondary Structure Alphabet
- G: 3-turn helix (310helix)
- H: α-helix
- I: 𝝅-helix (5 - turn helix)
- T: Hydrogen Bonded Turn
- B: β-sheet
- S: Bend
- C: Coil (residues not present in any of the above conformations)
- N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. |
Noma A, Kirino Y, Ikeuchi Y, Suzuki T |
EMBO J |
[details]
|
16642040
|
-
|
| A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA. |
Kalhor HR, Penjwini M, Clarke S |
Biochem Biophys Res Commun |
[details]
|
16005430
|
-
|
Links: