Full name: H/ACA ribonucleoprotein complex subunit 4
Synonym: dyskerine
GI: 461698
Orf: YLR175W
COG: COG0130
UniProt: P33322
Structures: | 3UAI | 3U28 |
Complex: H/ACA RNP
Enzyme type: pseudouridine synthase
Position of modification - modification: t:many - Y
s:many - Y
l:many - Y
n:many - Y


Eukaryal Cbf5 is the catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNPs) complex. Catalyzes the pseudouridylation of rRNA (including 5S and 5.8S rRNA) and small RNAs (snRNAs, snoRNAs). Contains one PUA domain. Works with 3 other subunits Gar1p, Nop10p, Nhp2p. A fifth protein (Shq1) is required for H/ACA RNP biogenenesis. In Eukarya, H/ACA RNPs are essential for three fundamental cellular processes: protein synthesis, mRNA splicing, and maintenance of genome integrity. For detailed information on modified positions see the snoRNA table ( http://modomics.genesilico.pl/snorna/ ).

Protein sequence:


Enzymatic activities:

Reaction Substrate Type Position
U:Y rRNA (r) SSU/18S/cytosolic many
U:Y rRNA (r) LSU-L/25S/cytosolic many
U:Y pre-rRNA (pre-r) all/35S/prokaryotic cytosol many
U:Y snRNA (sn) all/all/cytosolic many


Title Authors Journal Details PubMed Id DOI
An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules. Jiang W, Middleton K, Yoon HJ, Fouquet C, Carbon J Mol Cell Biol [details] 8336724 -
The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs. Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR Nat Struct Mol Biol [details] 16286935 -
Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita. Rashid R, Liang B, Baker DL, Youssef OA, He Y, Phipps K, Terns RM, Terns MP, Li H Mol Cell [details] 16427014 -
The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Lafontaine DL, Bousquet-Antonelli C, Henry Y, Caizergues-Ferrer M, Tollervey D Genes Dev [details] 9472021 -
Point mutations in yeast CBF5 can abolish in vivo pseudouridylation of rRNA. Zebarjadian Y, King T, Fournier MJ, Clarke L, Carbon J Mol Cell Biol [details] 10523634 -
Structure of the Shq1-Cbf5-Nop10-Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita. Li S, Duan J, Li D, Ma S, Ye K EMBO J [details] 22117216 -
Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs. Henras AK, Capeyrou R, Henry Y, Caizergues-Ferrer M RNA [details] 15388873 -
The box H/ACA RNP assembly factor Naf1p contains a domain homologous to Gar1p mediating its interaction with Cbf5p. Leulliot N, Godin KS, Hoareau-Aveilla C, Quevillon-Cheruel S, Varani G, Henry Y, Van Tilbeurgh H... J Mol Biol [details] 17612558 -


_The yeast snoRNA database_
_Wikipedia - Small nucleolar RNA_
_Wikipedia - Pseudouridylate synthase_
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