Modomics - A Database of RNA Modifications

Full name:	tRNA-dihydrouridine synthase 1
Synonym:
GI:	1730621
Orf:	YML080W
COG:	COG0042
UniProt:	P53759
Structures:	\| \|
Complex:
Enzyme type:	dihydrouridine synthase
Position of modification - modification:	t:17 - D t:16 - D

Comments:

Dus1 catalyzes the D formation of tRNA through reduction of uracil base with flavin mononucleotide (FMN) as a cofactor. Not essential enzyme.

Protein sequence:

MTEPALSSANNALMQKLTGRQLFDKIGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDGSSVDRPLVVQFCANDPEY
LLAAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLIHNLINTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTG
LANWETIKYLRDNLPKETVFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQTKNKEKIFPRVDKIIREYFQIVKECQESKASKTAMKSHF
FKILRPFLPHHTDIRSTLATMNAKATWEEWEEQVVKPVEKVVQEIFEQPDIAIKDEITIGEKQSWGGSYRTVPYWRCQPYFRPVNGITGDKRVMQGLIDE
SVNKKRKADVPLESADKKKDVKA

Enzymatic activities:

Reaction	Substrate	Type	Position
U:D	tRNA (t)	Ala/IGC/prokaryotic cytosol	16
U:D	tRNA (t)	Arg/ACU/prokaryotic cytosol	16
U:D	tRNA (t)	Arg/ICG/prokaryotic cytosol	16
U:D	tRNA (t)	Asn/GUU/prokaryotic cytosol	16
U:D	tRNA (t)	Asp/GUC/prokaryotic cytosol	16
U:D	tRNA (t)	Cys/GCA/prokaryotic cytosol	16
U:D	tRNA (t)	Gly/GCC/prokaryotic cytosol	16
U:D	tRNA (t)	Gly/{CC/prokaryotic cytosol	16
U:D	tRNA (t)	His/GUG/prokaryotic cytosol	16
U:D	tRNA (t)	Ile/IAU/prokaryotic cytosol	16
U:D	tRNA (t)	Ile/UAU/prokaryotic cytosol	16
U:D	tRNA (t)	Leu/UAG/prokaryotic cytosol	16
U:D	tRNA (t)	Leu/UAA/prokaryotic cytosol	16
U:D	tRNA (t)	Lys/CUU/prokaryotic cytosol	16
U:D	tRNA (t)	Lys/SUU/prokaryotic cytosol	16
U:D	tRNA (t)	Met/CAU/prokaryotic cytosol	16
U:D	tRNA (t)	Phe/GAA/prokaryotic cytosol	16
U:D	tRNA (t)	Pro/UGG/prokaryotic cytosol	16
U:D	tRNA (t)	Ser/CGA/prokaryotic cytosol	16
U:D	tRNA (t)	Ser/UGA/prokaryotic cytosol	16
U:D	tRNA (t)	Ser/IGA/prokaryotic cytosol	16
U:D	tRNA (t)	Thr/IGU/prokaryotic cytosol	16
U:D	tRNA (t)	Trp/CCA/prokaryotic cytosol	16
U:D	tRNA (t)	Tyr/GUA/prokaryotic cytosol	16
U:D	tRNA (t)	Val/IAC/prokaryotic cytosol	16
U:D	tRNA (t)	Asn/GUU/prokaryotic cytosol	17
U:D	tRNA (t)	Ile/IAU/prokaryotic cytosol	17
U:D	tRNA (t)	Lys/CUU/prokaryotic cytosol	17
U:D	tRNA (t)	Phe/GAA/prokaryotic cytosol	17
U:D	tRNA (t)	Thr/IGU/prokaryotic cytosol	17
U:D	tRNA (t)	Arg/UCU/mitochondrion	16
U:D	tRNA (t)		16

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs.	Xing F, Hiley SL, Hughes TR, Phizicky EM	J Biol Chem	[details]	14970222	-
A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA.	Xing F, Martzen MR, Phizicky EM	RNA	[details]	12003496	-
Molecular evolution of dihydrouridine synthases.	Kasprzak JM, Czerwoniec A, Bujnicki JM...	BMC Bioinformatics	[details]	22741570	-

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