Modomics - A Database of RNA Modifications

Full name:	tRNA-dihydrouridine synthase 3
Synonym:
GI:	547573
Orf:	YLR401C
COG:
UniProt:	Q06053
Structures:	\| \|
Complex:
Enzyme type:	dihydrouridine synthase
Position of modification - modification:	t:47 - D

Comments:

Dus3 catalyzes the D formation of tRNA through reduction of uracil base with flavin mononucleotide (FMN) as a cofactor. Not essential enzyme. Dus3 modifies all tRNAs with U at position 47, with the exception of tRNAPhe1, Phe2, and Lys1.

Protein sequence:

MEQNAEKRSIVGDDNSTVKRQDTSPSKGIAHIKPEYIVPLKQNENQKVAIYDEEMSSDRMTNEFAGGTNKKNKNGRGKKRGQNKNRDNRQVKEQNVLCPR
LIHGDISKCSFGDNCRFVHDINLYLSTKKPEVESNIFPSCPVFNSLGFCPMGFKCRFLSSHLNKEDNILISKKEIDPDAQTIWSVKGEVNHISPERKLDL
IKRRFPFTKSNEILEIIDSFQQECRDSMKPEEEVESTPQLKKQDPDVEQPVAPQVEQRNKELSEHRMKQREVYLKYKDTRYFAQEKKPLDLYHKKIVSPL
TTVGNLPYRRLMRKLGADVTYSEMALAVPLIQGTNSEWALPKAHTSEFPGFGVQVACSKAWQAAKAAEALANSVSEISEINLNSGCPIDLLYRQGSGSAL
LDNPARMIRCLNAMNYVSKDIPITVKIRTGTKEGHPIAEGLVKRLVNETDVAAITLHGRSRQQRYTKSADWDYVSQVADTLRSAEADFIETEQGKEGRDS
KNRIQFVGNGDVNNFEDWYRYLNGNENIDSVMVARGALIKPWIFEEVESQQYLDKTSTERLDILRDYAQFSMEHWGTDEYGISQCRRFFCEFMSFFHRYV
PWVFVKDTL

Enzymatic activities:

Reaction	Substrate	Type	Position
U:D	tRNA (t)	Ala/IGC/prokaryotic cytosol	47
U:D	tRNA (t)	Arg/ICG/prokaryotic cytosol	47
U:D	tRNA (t)	Arg/ICU/prokaryotic cytosol	47
U:D	tRNA (t)	Asn/GUU/prokaryotic cytosol	47
U:D	tRNA (t)	Cys/GCA/prokaryotic cytosol	47
U:D	tRNA (t)	Ile/IAU/prokaryotic cytosol	47
U:D	tRNA (t)	Ile/UAU/prokaryotic cytosol	47
U:D	tRNA (t)	Lys/CUU/prokaryotic cytosol	47
U:D	tRNA (t)	Met/CAU/prokaryotic cytosol	47
U:D	tRNA (t)	Thr/IGU/prokaryotic cytosol	47
U:D	tRNA (t)	Trp/CCA/prokaryotic cytosol	47
U:D	tRNA (t)	Tyr/GUA/prokaryotic cytosol	47
U:D	tRNA (t)	Val/UAC/prokaryotic cytosol	47
U:D	tRNA (t)	Val/CAC/prokaryotic cytosol	47
U:D	tRNA (t)	Val/IAC/prokaryotic cytosol	47
U:D	tRNA (t)		47

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs.	Xing F, Hiley SL, Hughes TR, Phizicky EM	J Biol Chem	[details]	14970222	-
A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA.	Xing F, Martzen MR, Phizicky EM	RNA	[details]	12003496	-
Molecular evolution of dihydrouridine synthases.	Kasprzak JM, Czerwoniec A, Bujnicki JM...	BMC Bioinformatics	[details]	22741570	-

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