Modomics - A Database of RNA Modifications
MnmCD from Escherichia coli - protein summary.
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase / FAD-dependent cmnm(5)s(2)U34 oxidoreductase
Position of modification - modification:
t:34 - mnm5s2U
t:34 - mnm5U
In E.coli (and many other bacteria) it is a bifunctional enzyme, composed of two domains that catalyze two successive reactions at the uridine wobble position 34 in tRNA: first a deacetylation reaction of cmnm5U into nm5U, then methylation of the amine group of nm5U to form mnm5U. In other organisms they may be unlinked (separate proteins), then identified as MnmC (former MnmC1) for the deacetylase and MnmD (former MnmC2) for the methyltransferase. In certain bacteria, the catalytic domain for the deacetylation reaction is absent in their genome and only the gene coding for MnmD is present. In other bacteria, MnmC and MnmD are both present. In E. coli MnmCD the two domains act independently. For tRNALeucmnm5Um the activity of methyltransferase domain was observed only in vitro.
Identification of a bifunctional enzyme MnmC involved in the biosynthesis of a hypermodified uridine in the wobble position of tRNA.
Bujnicki JM, Oudjama Y, Roovers M, Owczarek S, Caillet J, Droogmans L
Sequence-structure-function analysis of the bifunctional enzyme MnmC that catalyses the last two steps in the biosynthesis of hypermodified nucleoside mnm5s2U in tRNA.
Roovers M, Oudjama Y, Kaminska KH, Purta E, Caillet J, Droogmans L, Bujnicki JM
Assay of both activities of the bifunctional tRNA-modifying enzyme MnmC reveals a kinetic basis for selective full modification of cmnm5s2U to mnm5s2U.
Pearson D, Carell T
Nucleic Acids Res
Crystal structure of the bifunctional tRNA modification enzyme MnmC from Escherichia coli.
Kitamura A, Sengoku T, Nishimoto M, Yokoyama S, Bessho Y
Structural basis for hypermodification of the wobble uridine in tRNA by bifunctional enzyme MnmC.
Kim J, Almo SC
BMC Struct Biol
The output of the tRNA modification pathways controlled by the Escherichia coli MnmEG and MnmC enzymes depends on the growth conditions and the tRNA species.
Moukadiri I, Garzon MJ, Bjork GR, Armengod ME...
Nucleic Acids Res
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