ID Card:
Full name:
Mitochondrial translation optimization protein 1
Synonym:
Ips1
GI:
115502421
Orf:
YGL236C
COG:
COG0445
UniProt:
P53070
Alpha Fold Predicted Structure:
AF-P53070-F1
Complex:
Mto1/Mss1
Enzyme type:
other
Position of modification - modification:
t :34 - cmnm5s2U
Protein sequence:
MLRVTTLASSCTSFPLQVLRRRLTISSLTSFQPTTKTQVVVIGAGHAGCEAAAASSRTGAHTTLITPSLTDIGKCSCNPSIGGVGKGILVKEIDALDGLMGKVTDLAGVQFKMLNRSKGPAVWGPRAQIDRELYKKYMQRELSDKKAHPNLSLLQNKVADLILYDPGCGHKVIKGVVLDDGTQVGADQVIITTGTFLSAEIHIGDKRIAAGRIGEQPTYGISNTLQNEVGFQLGRLKTGTPARLAKESIDFSALEVQKGDALPVPMSFLNETVSVEPTKQLDCFGTHTTPQMHDFLRNNLHQSIHIQDTTIKGPRYCPSIEAKILRFPDRSSHKIWLEPEGFNSDVIYPNGISNSMPEDVQLQMMRLIPGMANVEILQPAYGVEYDYVDPRQLKPSLETKLVDGLFLAGQINGTTGYEEAAAQGIIAGINAGLLSRQEREQLVLKRSEAYIGVLIDDLINNGVIEPYRMFTSRSEFRISVRADNADFRLTPIGAQLGIISPVRLSQYSRDKHLYDETIRALQNFKLSSQKWSSLLQANIAPQAENRSAWEIFRFKDMDLHKLYECIPDLPINLLDIPMHVVTKINIQGKYEPYIVKQNQFVKAFQADENMLLPQDYDYRQLPTLSTECKLLLNRVQPLTIGQARRIQGITAAALFELYRVARKPSQPVM
Comments:
Yeast mitochondrial homolog of bacterial MnmG (GidA). Favoprotein. Makes a stable complex with Mss1p. Does not work on cytoplasmic tRNAs. Belongs to the bacterial gidA family but is distinct from bacterial Gid, renamed TrmFO.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
s2U:cmnm5s2U
RNA
tRNA
34
2UU
$UU
tRNALeu 2AA
wobble - position
Mitochondrion
15509579
U:cmnm5U
RNA
tRNA
34
UUU
!UU
tRNALys UUU
wobble - position
Mitochondrion
Alpha Fold Predicted Structure:
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Protein sequence:
M
L
R
V
T
T
L
A
S
S
C
T
S
F
P
L
Q
V
L
R
R
R
L
T
I
S
S
L
T
S
F
Q
P
T
T
K
T
Q
V
V
V
I
G
A
G
H
A
G
C
E
A
A
A
A
S
S
R
T
G
A
H
T
T
L
I
T
P
S
L
T
D
I
G
K
C
S
C
N
P
S
I
G
G
V
G
K
G
I
L
V
K
E
I
D
A
L
D
G
L
M
G
K
V
T
D
L
A
G
V
Q
F
K
M
L
N
R
S
K
G
P
A
V
W
G
P
R
A
Q
I
D
R
E
L
Y
K
K
Y
M
Q
R
E
L
S
D
K
K
A
H
P
N
L
S
L
L
Q
N
K
V
A
D
L
I
L
Y
D
P
G
C
G
H
K
V
I
K
G
V
V
L
D
D
G
T
Q
V
G
A
D
Q
V
I
I
T
T
G
T
F
L
S
A
E
I
H
I
G
D
K
R
I
A
A
G
R
I
G
E
Q
P
T
Y
G
I
S
N
T
L
Q
N
E
V
G
F
Q
L
G
R
L
K
T
G
T
P
A
R
L
A
K
E
S
I
D
F
S
A
L
E
V
Q
K
G
D
A
L
P
V
P
M
S
F
L
N
E
T
V
S
V
E
P
T
K
Q
L
D
C
F
G
T
H
T
T
P
Q
M
H
D
F
L
R
N
N
L
H
Q
S
I
H
I
Q
D
T
T
I
K
G
P
R
Y
C
P
S
I
E
A
K
I
L
R
F
P
D
R
S
S
H
K
I
W
L
E
P
E
G
F
N
S
D
V
I
Y
P
N
G
I
S
N
S
M
P
E
D
V
Q
L
Q
M
M
R
L
I
P
G
M
A
N
V
E
I
L
Q
P
A
Y
G
V
E
Y
D
Y
V
D
P
R
Q
L
K
P
S
L
E
T
K
L
V
D
G
L
F
L
A
G
Q
I
N
G
T
T
G
Y
E
E
A
A
A
Q
G
I
I
A
G
I
N
A
G
L
L
S
R
Q
E
R
E
Q
L
V
L
K
R
S
E
A
Y
I
G
V
L
I
D
D
L
I
N
N
G
V
I
E
P
Y
R
M
F
T
S
R
S
E
F
R
I
S
V
R
A
D
N
A
D
F
R
L
T
P
I
G
A
Q
L
G
I
I
S
P
V
R
L
S
Q
Y
S
R
D
K
H
L
Y
D
E
T
I
R
A
L
Q
N
F
K
L
S
S
Q
K
W
S
S
L
L
Q
A
N
I
A
P
Q
A
E
N
R
S
A
W
E
I
F
R
F
K
D
M
D
L
H
K
L
Y
E
C
I
P
D
L
P
I
N
L
L
D
I
P
M
H
V
V
T
K
I
N
I
Q
G
K
Y
E
P
Y
I
V
K
Q
N
Q
F
V
K
A
F
Q
A
D
E
N
M
L
L
P
Q
D
Y
D
Y
R
Q
L
P
T
L
S
T
E
C
K
L
L
L
N
R
V
Q
P
L
T
I
G
Q
A
R
R
I
Q
G
I
T
A
A
A
L
F
E
L
Y
R
V
A
R
K
P
S
Q
P
V
M
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae.
Colby G, Wu M, Tzagoloff A
J Biol Chem
[details]
9774408
-
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases.
Umeda N, Suzuki T, Yukawa M, Ohya Y, Shindo H, Watanabe K, Suzuki T
J Biol Chem
[details]
15509579
-
Links: