Modomics - A Database of RNA Modifications

Full name:	Mitochondrial translation optimization protein 1
Synonym:	Ips1
GI:	115502421
Orf:	YGL236C
COG:	COG0445
UniProt:	P53070
Structures:	\| \|
Complex:	Mto1/Mss1
Enzyme type:	other
Position of modification - modification:	t:34 - cmnm5s2U

Comments:

Yeast mitochondrial homolog of bacterial MnmG (GidA). Favoprotein. Makes a stable complex with Mss1p. Does not work on cytoplasmic tRNAs. Belongs to the bacterial gidA family but is distinct from bacterial Gid, renamed TrmFO.

Protein sequence:

MLRVTTLASSCTSFPLQVLRRRLTISSLTSFQPTTKTQVVVIGAGHAGCEAAAASSRTGAHTTLITPSLTDIGKCSCNPSIGGVGKGILVKEIDALDGLM
GKVTDLAGVQFKMLNRSKGPAVWGPRAQIDRELYKKYMQRELSDKKAHPNLSLLQNKVADLILYDPGCGHKVIKGVVLDDGTQVGADQVIITTGTFLSAE
IHIGDKRIAAGRIGEQPTYGISNTLQNEVGFQLGRLKTGTPARLAKESIDFSALEVQKGDALPVPMSFLNETVSVEPTKQLDCFGTHTTPQMHDFLRNNL
HQSIHIQDTTIKGPRYCPSIEAKILRFPDRSSHKIWLEPEGFNSDVIYPNGISNSMPEDVQLQMMRLIPGMANVEILQPAYGVEYDYVDPRQLKPSLETK
LVDGLFLAGQINGTTGYEEAAAQGIIAGINAGLLSRQEREQLVLKRSEAYIGVLIDDLINNGVIEPYRMFTSRSEFRISVRADNADFRLTPIGAQLGIIS
PVRLSQYSRDKHLYDETIRALQNFKLSSQKWSSLLQANIAPQAENRSAWEIFRFKDMDLHKLYECIPDLPINLLDIPMHVVTKINIQGKYEPYIVKQNQF
VKAFQADENMLLPQDYDYRQLPTLSTECKLLLNRVQPLTIGQARRIQGITAAALFELYRVARKPSQPVM

Enzymatic activities:

Reaction	Substrate	Type	Position
s2U:cmnm5s2U	tRNA (t)	Leu/UAA/mitochondrion	34
U:cmnm5U	tRNA (t)		34

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae.	Colby G, Wu M, Tzagoloff A	J Biol Chem	[details]	9774408	-
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases.	Umeda N, Suzuki T, Yukawa M, Ohya Y, Shindo H, Watanabe K, Suzuki T	J Biol Chem	[details]	15509579	-

Links:

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