Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase
GI: 67471968
Orf: b0405
COG: COG0809
UniProt: P0A7F9
Alpha Fold Predicted Structure: AF-P0A7F9-F1
Enzyme type: isomerase
Position of modification - modification: t:34 - Q



Protein sequence:

MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE

Comments:

AdoMet is the methyl group donor. Ribosyltransferase.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
preQ1:oQ RNA tRNA 34 ∉UA ςUA tRNATyrGUA wobble - position Prokaryotic Cytosol

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M R V T D F S F E L P E S L I A H Y P M P E R S S C R L L S L D G P T G A L T H G T F T D L L D K L N P G D L L V F N N T R V I P A R L F G R K A S G G K I E V L V E R M L D D K R I L A H I R A S K A P K P G A E L L L G D D E S I N A T M T A R H G A L F E V E F N D E R S V L D I L N S I G H M P L P P Y I D R P D E D A D R E L Y Q T V Y S E K P G A V A A P T A G L H F D E P L L E K L R A K G V E M A F V T L H V G A G T F Q P V R V D T I E D H I M H S E Y A E V P Q D V V D A V L A A K A R G N R V I A V G T T S V R S L E S A A Q A A K N D L I E P F F D D T Q I F I Y P G F Q Y K V V D A L V T N F H L P E S T L I M L V S A F A G Y Q H T M N A Y K A A V E E K Y R F F S Y G D A M F I T Y N P Q A I N E R V G E

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P0A7F9-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P0A7F9-F1.cif  
DSSP Secondary Structures   P0A7F9.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine. Slany RK, Bosl M, Crain PF, Kersten H Biochemistry [details] 8347586 -

Links:

_EcoCyc_