| Full name: |
rRNA methyltransferase, mitochondrial |
| Synonym: |
Pet56 |
| GI: |
1346886 |
| Orf: |
YOR201C |
| COG: |
COG0566 |
| UniProt: |
P25270 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
l:2270(2251) - Gm
|
Comments:
Mrm1 methylates the universally conserved G2270 in the so-called A-loop (hairpin 92) of the peptidyltransferase center (domain V) of 21S mito rRNA, equivalent to conserved Gm2251 in E. coli 23S rRNA and catalyzed by E. coli RlmB. The purified recombinant Mrm1 enzyme works in vitro on transcripts of both mitochondrial 21S rRNA and E. coli 23S rRNA. AdoMet is the donor of methyl group. In cytoplasmic S. cerevisiae rRNA, the analogous conserved Gm position 2618 (2251 E.coli numbering) is catalyzed at by a C/D box RNP (Nop1), guided by snoR67.
Protein sequence:
MTSLTNAVFKRYLAVTPSAHQALKTRIKKKSSSFDKFFPQQSNSRKKQWETLNEDKASWFKRKYAHVHAR
EQDRAADPYGKKKAHVEKLKEIKNQAKLNQKSHKSKFQNKDIALKLMNDNPIFEYVYGTNSVYAALLNPS
RNCHSRLLYHGTIPSKFLQIVDELKVTTELVDKHRLNLLTNYGVHNNIALETKPLQPVEIAYLGDMDTSS
AALSIHELGFNNENIPHELPYGTKTDAKKFPLGLYLDEITDPHNIGAIIRSAYFLGVDFIVMSRRNCSPL
TPVVSKTSSGALELLPIFYVDKPLEFFTKSQEMGGWTFITSHLANATSEKYTVGKTISMHDLNGLCNELP
VVLVVGNESQGVRTNLKMRSDFFVEIPFGGIEKGNRAPEPIVDSLNVSVATALLIDNILTCK
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
G:Gm
|
rRNA (r) |
LSU/21S/mitochondrial |
2270 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Functional requirement of a site-specific ribose methylation in ribosomal RNA. |
Sirum-Connolly K, Mason TL |
Science |
[details]
|
8266080
|
-
|
Links: