Modomics - A Database of RNA Modifications

ID Card:

Full name: Ribosomal RNA small subunit methyltransferase E
Synonym: YggJ
GI: 84027953
Orf: b2946
COG: COG1385
UniProt: P0AGL7
Alpha Fold Predicted Structure: AF-P0AGL7-F1
Enzyme type: methyltransferase
Position of modification - modification: s:1498(1498) - m3U



Protein sequence:

MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFGDLG

Comments:

Purified recombinant E. coli RsmE specifically methylates U1498 into m3U in vitro. This modified base is located in the loop of a conserved hairpin between helix 44 and 45, near the 3′-end of 16S rRNA. Recombinant RsmE is most active in the presence of 10-15 mM Mg2+ and 100 mM NH4Cl. While small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits can. This enzyme has an alpha/beta (trefoil) knot and forms dimers in solution.





Alpha Fold Predicted Structure:




Parsing response... [225003/225003]


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Protein sequence:

M R I P R I Y H P E P L T S H S H I A L C E D A A N H I G R V L R M G P G Q A L Q L F D G S N Q V F D A E I T S A S K K S V E V K V L E G Q I D D R E S P L H I H L G Q V M S R G E K M E F T I Q K S I E L G V S L I T P L F S E R C G V K L D S E R L N K K L Q Q W Q K I A I A A C E Q C G R N R V P E I R P A M D L E A W C A E Q D E G L K L N L H P R A S N S I N T L P L P V E R V R L L I G P E G G L S A D E I A M T A R Y Q F T D I L L G P R V L R T E T T A L T A I T A L Q V R F G D L G
20406080100120140160180200220240SequenceGHITSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P0AGL7-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P0AGL7-F1.cif  
DSSP Secondary Structures   P0AGL7.dssp  





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