| Full name: |
Ribosomal RNA small subunit methyltransferase E |
| Synonym: |
YggJ |
| GI: |
84027953 |
| Orf: |
b2946 |
| COG: |
COG1385 |
| UniProt: |
P0AGL7 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
s:1498(1498) - m3U
|
Comments:
Purified recombinant E. coli RsmE specifically methylates U1498 into m3U in vitro. This modified base is located in the loop of a conserved hairpin between helix 44 and 45, near the 3′-end of 16S rRNA. Recombinant RsmE is most active in the presence of 10-15 mM Mg2+ and 100 mM NH4Cl. While small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits can. This enzyme has an alpha/beta (trefoil) knot and forms dimers in solution.
Protein sequence:
MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFDGSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSI
ELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLS
ADEIAMTARYQFTDILLGPRVLRTETTALTAITALQVRFGDLG
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
U:m3U
|
rRNA (r) |
SSU/16S/prokaryotic cytosol |
1498 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family. |
Basturea GN, Rudd KE, Deutscher MP |
RNA |
[details]
|
16431987
|
-
|
| Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE. |
Basturea GN, Deutscher MP |
RNA |
[details]
|
17872509
|
-
|
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