Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA 2-thiocytidine(32) synthetase
Synonym: YdaO
GI: 2495598
Orf: b1344
COG: COG0037
UniProt: P76055
Alpha Fold Predicted Structure: AF-P76055-F1
Enzyme type: thiolase
Position of modification - modification: t:32 - s2C



Protein sequence:

MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPACWQPEEDENQLDELRLNVVEVK

Comments:

The synthesis of s2C requires IscS as a sulfur donor and belongs to the “iron-cluster” pathway (as MiaB). Much sequence similarities exist between bacterial TtcA and yeast Tuc1 responsible for the thiolation of s2U34 (convergent evolution). They might have evolved from a common thiolase precursor.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M Q E N Q Q I T K K E Q Y N L N K L Q K R L R R N V G E A I A D F N M I E E G D R I M V C L S G G K D S Y T M L E I L R N L Q Q S A P I N F S L V A V N L D Q K Q P G F P E H V L P E Y L E K L G V E Y K I V E E N T Y G I V K E K I P E G K T T C S L C S R L R R G I L Y R T A T E L G A T K I A L G H H R D D I L Q T L F L N M F Y G G K M K G M P P K L M S D D G K H I V I R P L A Y C R E K D I Q R F A D A K A F P I I P C N L C G S Q P N L Q R Q V I A D M L R D W D K R Y P G R I E T M F S A M Q N V V P S H L C D T N L F D F K G I T H G S E V V N G G D L A F D R E E I P L Q P A C W Q P E E D E N Q L D E L R L N V V E V K
50100150200250300SequenceGHITSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P76055-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P76055-F1.cif  
DSSP Secondary Structures   P76055.dssp  





Publications:

Links:

_EcoCyc_