The synthesis of s2C requires IscS as a sulfur donor and belongs to the “iron-cluster” pathway (as MiaB). Much sequence similarities exist between bacterial TtcA and yeast Tuc1 responsible for the thiolation of s2U34 (convergent evolution). They might have evolved from a common thiolase precursor.
The conserved Cys-X1-X2-Cys motif present in the TtcA protein is required for the thiolation of cytidine in position 32 of tRNA from Salmonella enterica serovar Typhimurium.
Jager G, Leipuviene R, Pollard MG, Qian Q, Bjork GR
A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast.
Iron-sulfur cluster biosynthesis in bacteria: Mechanisms of cluster assembly and transfer.
The conserved Cys-X1-X2-Cys motif present in the TtcA protein is required for the thiolation of cytidine in position 32 of tRNA from Salmonella enterica serovar Typhimurium.
TtcA a new tRNA-thioltransferase with an Fe-S cluster.