Modomics - A Database of RNA Modifications

ID Card:

Full name: RNA cap guanine-N7 methyltransferase
Synonym: MceS
GI: 416567
Orf: YBR1602
COG: COG2226
UniProt: P32783
Alpha Fold Predicted Structure: AF-P32783-F1
Enzyme type: methyltransferase
Position of modification - modification: m:0 - m7GpppN



Protein sequence:

MSTKPEKPIWMSQEDYDRQYGSITGDESSTVSKKDSKVTANAPGDGNGSLPVLQSSSILTSKVSDLPIEAESGFKIQKRRHERYDQEERLRKQRAQKLREEQLKRHEIEMTANRSINVDQIVREHYNERTIIANRAKRNLSPIIKLRNFNNAIKYMLIDKYTKPGDVVLELGCGKGGDLRKYGAAGISQFIGIDISNASIQEAHKRYRSMRNLDYQVVLITGDCFGESLGVAVEPFPDCRFPCDIVSTQFCLHYAFETEEKARRALLNVAKSLKIGGHFFGTIPDSEFIRYKLNKFPKEVEKPSWGNSIYKVTFENNSYQKNDYEFTSPYGQMYTYWLEDAIDNVPEYVVPFETLRSLADEYGLELVSQMPFNKFFVQEIPKWIERFSPKMREGLQRSDGRYGVEGDEKEAASYFYTMFAFRKVKQYIEPESVKPN

Comments:

AdoMet is the methyl group donor. Responsible for methylating the 5'cap structure of mRNAs. Nucleotide 0 is a cap. Beside its capping function, Abd1 has also a function in transcription by RNA polymerase II. This transcriptional function is independent of methylase activity. The m7G has a positively charge at neutral pH.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m7G RNA mRNA 1 Nucleus
GpppN:m7GpppN RNA mRNA 1 Nucleus 7623811   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M S T K P E K P I W M S Q E D Y D R Q Y G S I T G D E S S T V S K K D S K V T A N A P G D G N G S L P V L Q S S S I L T S K V S D L P I E A E S G F K I Q K R R H E R Y D Q E E R L R K Q R A Q K L R E E Q L K R H E I E M T A N R S I N V D Q I V R E H Y N E R T I I A N R A K R N L S P I I K L R N F N N A I K Y M L I D K Y T K P G D V V L E L G C G K G G D L R K Y G A A G I S Q F I G I D I S N A S I Q E A H K R Y R S M R N L D Y Q V V L I T G D C F G E S L G V A V E P F P D C R F P C D I V S T Q F C L H Y A F E T E E K A R R A L L N V A K S L K I G G H F F G T I P D S E F I R Y K L N K F P K E V E K P S W G N S I Y K V T F E N N S Y Q K N D Y E F T S P Y G Q M Y T Y W L E D A I D N V P E Y V V P F E T L R S L A D E Y G L E L V S Q M P F N K F F V Q E I P K W I E R F S P K M R E G L Q R S D G R Y G V E G D E K E A A S Y F Y T M F A F R K V K Q Y I E P E S V K P N

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P32783-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P32783-F1.cif  
DSSP Secondary Structures   P32783.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Yeast mRNA cap methyltransferase is a 50-kilodalton protein encoded by an essential gene. Mao X, Schwer B, Shuman S Mol Cell Biol [details] 7623811 -
mRNA:guanine-N7 cap methyltransferases: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure-function relationships. Bujnicki JM, Feder M, Radlinska M, Rychlewski L BMC Bioinformatics [details] 11472630 -
Mutational analysis of the Saccharomyces cerevisiae ABD1 gene: cap methyltransferase activity is essential for cell growth. Mao X, Schwer B, Shuman S Mol Cell Biol [details] 8552073 -
Structure-function analysis of the mRNA cap methyltransferase of Saccharomyces cerevisiae. Wang SP, Shuman S J Biol Chem [details] 9169431 -
Dynamic association of capping enzymes with transcribing RNA polymerase II. Schroeder SC, Schwer B, Shuman S, Bentley D Genes Dev [details] 11018011 -

Links:

_PubMed - Abd1 Saccharomyces cap_
_PubMed - Abd1 gene function_
_Wikipedia_