Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA(His) guanylyltransferase
GI: 45270428
Orf: YGR024c
COG: COG4021
UniProt: P53215
Structures: | |
Alpha Fold Predicted Structure: AF-P53215-F1
Enzyme type: guanylyltransferase
Position of modification - modification: t:0 - G



Protein sequence:

MANSKFGYVRQFETHDVILPQCYIVVRIDGKKFHEFSKFYEFAKPNDENALKLMNACAKNLVLKYKNDIILAFGESDEYSFILKSSTTLFNRRKDKLATLFGSFFTSNYVALWAKFFPEKPLNIKHLPYFDSRCVAYPNLQTIKDYLSWRYVDTHINNLYNTTFWQLIIKCGLTPQESEKKLCGTFSNEKQEILFSECGINYNNEPEMFKKGSLVTRKGEILHINVIAQIDELFEGY

Comments:

tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage. Enzymes from Thg1 family exhibit 3'-to-5' polymerase activity. A crystal structure of Mycobacterium tuberculosis and H. sapiens homologues have been solved (3OTB, 3OTD, 3OTE and 3OTC, respectively).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
pN:pG(pN) RNA tRNA 0 GUG GUG tRNAHisGUG acceptor-stem Cytoplasm 21059936   

Alpha Fold Predicted Structure:






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Protein sequence:

M A N S K F G Y V R Q F E T H D V I L P Q C Y I V V R I D G K K F H E F S K F Y E F A K P N D E N A L K L M N A C A K N L V L K Y K N D I I L A F G E S D E Y S F I L K S S T T L F N R R K D K L A T L F G S F F T S N Y V A L W A K F F P E K P L N I K H L P Y F D S R C V A Y P N L Q T I K D Y L S W R Y V D T H I N N L Y N T T F W Q L I I K C G L T P Q E S E K K L C G T F S N E K Q E I L F S E C G I N Y N N E P E M F K K G S L V T R K G E I L H I N V I A Q I D E L F E G Y

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P53215-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P53215-F1.cif  
DSSP Secondary Structures   P53215.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis. Gu W, Jackman JE, Lohan AJ, Gray MW, Phizicky EM Genes Dev [details] 14633974 -
tRNAHis guanylyltransferase (THG1), a unique 3′-5′ nucleotidyl transferase, shares unexpected structural homology with canonical 5′-3′ DNA polymerases. Hyde SJ, Eckenroth BE, Smith BA, Eberley WA, Heintz NH, Jackman JE, Doublié S Proc Natl Acad Sci U S A [details] 21059936 -
Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily. Jackman JE, Gott JM, Gray MW RNA [details] 22456265 -

Links:

_PubMed_
_Wikipedia - Guanylyl transferase_
_SGD_