Modomics - A Database of RNA Modifications

ID Card:

Full name: Mitochondrial tRNA-specific 2-thiouridylase 1
Synonym: Mto2, SLM3
GI: 6320172
Orf: D2761
COG: COG0482
UniProt: Q12093
Structures: | |
Alpha Fold Predicted Structure: AF-Q12093-F1
Enzyme type: sulfurtransferase
Position of modification - modification: t:34 - cmnm5s2U



Protein sequence:

MLARYLNLIGRRSASPYRPQRLPAKFDNVIVAMSSGVDSSVAAALFAGEFPNTRGVYMQNWSESQSLDDPGKEPCYERDWRDVNRVAKHLNIRVDKVNFEQDYWIDVFEPMLRGYSEGSTPNPDIGCNKFVKFGKLREWLDEKYGTGNYWLVTGHYARVMQEMNGKGLFHLLRSIYRPKDQSYYLSQINSTVLSSLLLPIGHLTKPEVRDLAKYAGLPTAEKPDSQGICFVNNSQHGKFKNFLKHYLPSSPGDIITVDPQSGAKTTWGRHDGLWSYTIGQKVGISMPQADPNYQGTWFVSEKLRDTNEILIVRGRDNPALYSDTMRIENFSSLGPREDTINAFQNTGALTLQFRSLQVPVQIKSCKLNRSADNLDITIHLASKQRAITPGQSCCLYIDDRVLGSGPISHVNNNDTHA

Comments:

Homolog of bacterial ATP-dependent tRNA sulphur transferase MnmA (alias TrmU). Sulfur group originates from cysteine via a cascade of sulphur relay proteins, of which the pyridoxal phosphate-containing cysteine desulfurase Nfs1 plays the same role as IscS in bacteria. At least one other mitochondrial sulfur transport protein remains to be identified (2012). U34 thiolation in mitochondria differs from that in bacteria, which involves IscS (instead of Nfs1) and a cascade of relay proteins TusA,B,C,D,E.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
cmnm5U:cmnm5s2U RNA tRNA 34 !UU $UU tRNALysUUU Mitochondrion 15509579   
U:s2U RNA tRNA 34 UUU 2UU tRNALysUUU Mitochondrion
nm5U:nm5s2U RNA tRNA 34 ∪UU ∫UU tRNALysUUU Mitochondrion
mnm5U:mnm5s2U RNA tRNA 34 {UU SUU tRNALysUUU Mitochondrion

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M L A R Y L N L I G R R S A S P Y R P Q R L P A K F D N V I V A M S S G V D S S V A A A L F A G E F P N T R G V Y M Q N W S E S Q S L D D P G K E P C Y E R D W R D V N R V A K H L N I R V D K V N F E Q D Y W I D V F E P M L R G Y S E G S T P N P D I G C N K F V K F G K L R E W L D E K Y G T G N Y W L V T G H Y A R V M Q E M N G K G L F H L L R S I Y R P K D Q S Y Y L S Q I N S T V L S S L L L P I G H L T K P E V R D L A K Y A G L P T A E K P D S Q G I C F V N N S Q H G K F K N F L K H Y L P S S P G D I I T V D P Q S G A K T T W G R H D G L W S Y T I G Q K V G I S M P Q A D P N Y Q G T W F V S E K L R D T N E I L I V R G R D N P A L Y S D T M R I E N F S S L G P R E D T I N A F Q N T G A L T L Q F R S L Q V P V Q I K S C K L N R S A D N L D I T I H L A S K Q R A I T P G Q S C C L Y I D D R V L G S G P I S H V N N N D T H A

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q12093-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q12093-F1.cif  
DSSP Secondary Structures   Q12093.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. Umeda N, Suzuki T, Yukawa M, Ohya Y, Shindo H, Watanabe K, Suzuki T J Biol Chem [details] 15509579 -
Deletion of the MTO2 gene related to tRNA modification causes a failure in mitochondrial RNA metabolism in the yeast Saccharomyces cerevisiae. Wang X, Yan Q, Guan MX FEBS Lett [details] 17706197 -
Mutations in MTO2 related to tRNA modification impair mitochondrial gene expression and protein synthesis in the presence of a paromomycin resistance mutation in mitochondrial 15 S rRNA. Yan Q, Li X, Faye G, Guan MX J Biol Chem [details] 15944150 -

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