Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA wyosine derivatives biosynthesis protein Taw1
Synonym: MjTYW1
GI: 2501613
Orf: MJ0257
COG: COG0731
UniProt: Q57705
Structures: | |
Alpha Fold Predicted Structure: AF-Q57705-F1
Enzyme type: oxidoreductase



Protein sequence:

MIPEEIYKILRKQRYQIDGHTAVKLCGWVRKKMLEDKNCYKSKFYGIETHRCIQCTPSVIWCQQNCIFCWRVLPRDIGIDISQIKEPKWEEPEVVYEKILAMHKRIIMGYAGVLDRVGEKKFKEALEPKHVAISLSGEPTLYPYLDELIKIFHKNGFTTFVVSNGILTDVIEKIEPTQLYISLDAYDLDSYRRICGGKKEYWESILNTLDILKEKKRTCIRTTLIRGYNDDILKFVELYERADVHFIELKSYMHVGYSQKRLKKEDMLQHDEILKLAKMLDENSSYKLIDDSEDSRVALLQNENRKINPKL

Comments:

Homologue of yeast Tyw1. Does not have the N-terminal FMN binding/flavodoxin domain found in Tyw1.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
m1G:imG-14 RNA tRNA 37 GAA GAA tRNAPheGAA anticodon-loop Prokaryotic Cytosol 22026549   

Alpha Fold Predicted Structure:






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Protein sequence:

M I P E E I Y K I L R K Q R Y Q I D G H T A V K L C G W V R K K M L E D K N C Y K S K F Y G I E T H R C I Q C T P S V I W C Q Q N C I F C W R V L P R D I G I D I S Q I K E P K W E E P E V V Y E K I L A M H K R I I M G Y A G V L D R V G E K K F K E A L E P K H V A I S L S G E P T L Y P Y L D E L I K I F H K N G F T T F V V S N G I L T D V I E K I E P T Q L Y I S L D A Y D L D S Y R R I C G G K K E Y W E S I L N T L D I L K E K K R T C I R T T L I R G Y N D D I L K F V E L Y E R A D V H F I E L K S Y M H V G Y S Q K R L K K E D M L Q H D E I L K L A K M L D E N S S Y K L I D D S E D S R V A L L Q N E N R K I N P K L

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q57705-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q57705-F1.cif  
DSSP Secondary Structures   Q57705.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA. Suzuki Y, Noma A, Suzuki T, Senda M, Senda T, Ishitani R, Nureki O J Mol Biol [details] 17727881 -
Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea. de Crecy-Lagard V, Brochier-Armanet C, Urbonavicius J, Fernandez B, Phillips G, Lyons B, Noma A, Alvarez S, Droogmans L, Armengaud J, Grosjean H Mol Biol Evol [details] 20382657 -
Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine. Young AP, Bandarian V... Biochemistry [details] 22026549 -