Modomics - A Database of RNA Modifications

Full name:	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Synonym:	GidA
GI:	21675089
Orf:
COG:	COG0445
UniProt:	Q8KA85
Structures:	\| 3CP8 \|
Complex:	MnmG/MnmE
Enzyme type:	other
Position of modification - modification:	t:34 - cmnm5s2U t:34 - cmnm5U

Comments:

Homologue of E. coli MnmG. Flavoprotein, using methyl-(or methylen)-tetrahydrofolate as a donor of one carbon. In a complex with GTPase MnmE they catalyze the formation of either cmnm5U or nm5U by reacting either with glycine or with ammonium.

Protein sequence:

MYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRMLNRSKGPAMHSPRAQADKTQYS
LYMRRIVEHEPNIDLLQDTVIGVSANSGKFSSVTVRSGRAIQAKAAILACGTFLNGLIHIGMDHFPGGRSTAEPPVEGLTESLASLGFSFGRLKTGTPPR
IDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYLTKTTEKTHDILRTGFDRSPLFTGKVQGVGPRYCPSIEDKISRFPDKSSHHIFLEPEGTDT
VEMYVNGFSTSLPEDIQIAGLRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPVENLFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLG
RDQAYIGVLIDDLITKETKEPYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDDLHRTESIIKRVQHCLEVMKTAKVTPAEINTLLMNKGLQELKT
PARALSLIKRPGISLQDILEHSLSVRSAAEELCNDPRVAEQVQIEIKYEGYIKREQLVADRIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQ
ASRILGVSPSDVSILMIRLGR

Enzymatic activities:

Reaction	Substrate	Position
U:cmnm5U	tRNA (t)	34
U:nm5U	tRNA (t)	34
s2U:nm5s2U	tRNA (t)	34
se2U:nm5se2U	tRNA (t)	34
s2U:cmnm5s2U	tRNA (t)	34
se2U:cmnm5se2U	tRNA (t)	34
Um:cmnm5Um	tRNA (t)	34

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate.	Meyer S, Scrima A, Versées W, Wittinghofer A	J Mol Biol	[details]	18565343	-
Enzymology of tRNA modification in the bacterial MnmEG pathway.	Armengod ME, Moukadiri I, Prado S, Ruiz-Partida R, Benitez-Paez A, Villarroya M, Lomas R, Garzon MJ, Martinez-Zamora A, Meseguer S, Navarro-Gonzalez C	Biochimie	[details]	22386868	-

Links:

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