Modomics - A Database of RNA Modifications

Full name:	tRNA modification GTPase MnmE
Synonym:	ThdF, TrmE
GI:	21674894
Orf:
COG:	COG0486
UniProt:	Q8KAS1
Structures:	\| 3GEE \| 3GEI \|
Complex:	MnmE/MnmG
Enzyme type:	GTPase
Position of modification - modification:	t:34 - cmnm5s2U t:34 - cmnm5U

Comments:

Homologue of E. coli MnmE. Hydrolyses GTP (contains a P-loop domain). MnmE works as dimer in complex with MnmG (GidA also as dimer). Catalyzes two independent reactions according to substrate (glycine or ammonium).

Protein sequence:

MSPSDLHLPVPGHPIAAIATPVGVGALAIVRISGAGVLDLADRVFRKVHGSGKLAEAAGYTAHFGRLYDGEEMVDEVIALVFRAPRSFTAEQMVEFTCHG
GPVVVGRVLRLMLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARTESAYRTAVSQMKGDLSVRLGGLREQLIRSCALIELELDFSEEDVEFQSR
DELTMQIETLRSEVNRLIDSYQHGRIVSEGVSTVIAGKPNAGKSTLLNTLLGQERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLREAGEEIEHEGI
RRSRMKMAEADLILYLLDLGTERLDDELTEIRELKAAHPAAKFLTVANKLDRAANADALIRAIADGTGTEVIGISALNGDGIDTLKQHMGDLVKNLDKLH
EASVLVTSLRHYEALRNASDALQNALELIAHESETELIAFELRAALDYVGQITGKVVNEEVLNTIFDKFCIGK

Enzymatic activities:

Reaction	Substrate	Position
Um:cmnm5Um	tRNA (t)	34
s2U:nm5s2U	tRNA (t)	34
se2U:nm5se2U	tRNA (t)	34
s2U:cmnm5s2U	tRNA (t)	34
se2U:cmnm5se2U	tRNA (t)	34
U:nm5U	tRNA (t)	34
U:cmnm5U	tRNA (t)	34

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy.	Meyer S, Bohme S, Kruger A, Steinhoff HJ, Klare JP, Wittinghofer A	PLoS Biol	[details]	19806182	-
Enzymology of tRNA modification in the bacterial MnmEG pathway.	Armengod ME, Moukadiri I, Prado S, Ruiz-Partida R, Benitez-Paez A, Villarroya M, Lomas R, Garzon MJ, Martinez-Zamora A, Meseguer S, Navarro-Gonzalez C	Biochimie	[details]	22386868	-

Links:

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