Modomics - A Database of RNA Modifications

Full name:	tRNA modification GTPase MnmE
Synonym:	TrmE
GI:	15643037
Orf:
COG:	COG0486
UniProt:	Q9WYA4
Structures:	\| 1XZP \| 1XZQ \|
Complex:	MnmE/MnmG
Enzyme type:	GTPase
Position of modification - modification:	t:34 - cmnm5s2U t:34 - cmnm5U

Comments:

Homologue of E. coli MnmE. Hydrolyses GTP. MnmE works in complex with MnmG (GidA). Catalyzes two independent reactions according to substrate (glycine or ammonium).

Protein sequence:

MDTIVAVATPPGKGAIAILRLSGPDSWKIVQKHLRTRSKIVPRKAIHGWIHENGEDVDEVVVVFYKSPKSYTGEDMVEVMCHGGPLVVKKLLDLFLKSGA
RMAEPGEFTKRAFLNGKMDLTSAEAVRDLIEAKSETSLKLSLRNLKGGLRDFVDSLRRELIEVLAEIRVELDYPDEIETNTGEVVTRLERIKEKLTEELK
KADAGILLNRGLRMVIVGKPNVGKSTLLNRLLNEDRAIVTDIPGTTRDVISEEIVIRGILFRIVDTAGVRSETNDLVERLGIERTLQEIEKADIVLFVLD
ASSPLDEEDRKILERIKNKRYLVVINKVDVVEKINEEEIKNKLGTDRHMVKISALKGEGLEKLEESIYRETQEIFERGSDSLITNLRQKQLLENVKGHLE
DAIKSLKEGMPVDMASIDLERALNLLDEVTGRSFREDLLDTIFSNFCVGK

Enzymatic activities:

Reaction	Substrate	Position
Um:cmnm5Um	tRNA (t)	34
s2U:nm5s2U	tRNA (t)	34
se2U:nm5se2U	tRNA (t)	34
s2U:cmnm5s2U	tRNA (t)	34
se2U:cmnm5se2U	tRNA (t)	34
U:nm5U	tRNA (t)	34
U:cmnm5U	tRNA (t)	34

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The structure of the TrmE GTP-binding protein and its implications for tRNA modification.	Scrima A, Vetter IR, Armengod ME, Wittinghofer A	EMBO J	[details]	15616586	-
Characterization of GTPase activity of TrmE, a member of a novel GTPase superfamily, from Thermotoga maritima.	Yamanaka K, Hwang J, Inouye M	J Bacteriol	[details]	11092873	-
Enzymology of tRNA modification in the bacterial MnmEG pathway.	Armengod ME, Moukadiri I, Prado S, Ruiz-Partida R, Benitez-Paez A, Villarroya M, Lomas R, Garzon MJ, Martinez-Zamora A, Meseguer S, Navarro-Gonzalez C	Biochimie	[details]	22386868	-

Links:

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