Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA-specific 2-thiouridylase mnmA
Synonym: TrmU
GI: 15900060
COG: COG0482
UniProt: Q97T38
Structures: | 2HMA |
Alpha Fold Predicted Structure: AF-Q97T38-F1
Enzyme type: thiolase
Position of modification - modification: t:34 - s2U
t:34 - mnm5s2U


PDB Structures:


2HMA

Structure Description:

Title: The Crystal Structure of tRNA (5-Methylaminomethyl-2-Thiouridylate)-Methyltransferase TrmU from Streptococcus pneumoniae
Classification: TRANSFERASE
Technique: X-Ray Diffraction
Resolution: 2.41
R value free: 0.217
R value observed: 0.193
R value work: 0.19

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   2HMA.pdb  
Pdbx/mmCIF Files   2HMA.cif  


Protein sequence:

MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTDENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLSQEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQPGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQGFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFAEPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI

Comments:

Homologue of E. coli MnmA and yeast or human mitochondrial TrmU (Mtu1). Requires cysteine desulfurase IscS as a sulfur donor and sulfur mediators: TusA, TusBCD and TusE. For more details see the entry for E. coli MnmA.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M S D N S K T R V V V G M S G G V D S S V T A L L L K E Q G Y D V I G I F M K N W D D T D E N G V C T A T E D Y K D V V A V A D Q I G I P Y Y S V N F E K E Y W D R V F E Y F L A E Y R A G R T P N P D V M C N K E I K F K A F L D Y A I T L G A D Y V A T G H Y A R V A R D E D G T V H M L R G V D N G K D Q T Y F L S Q L S Q E Q L Q K T M F P L G H L E K P E V R R L A E E A G L S T A K K K D S T G I C F I G E K N F K N F L S N Y L P A Q P G R M M T V D G R D M G E H A G L M Y Y T I G Q R G G L G I G G Q H G G D N A P W F V V G K D L S K N I L Y V G Q G F Y H D S L M S T S L E A S Q V H F T R E M P E E F T L E C T A K F R Y R Q P D S K V T V H V K G E K T E V I F A E P Q R A I T P G Q A V V F Y D G E E C L G G G L I D N A Y R D G Q V C Q Y I
50100150200250300350SequenceGHITBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q97T38-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q97T38-F1.cif  
DSSP Secondary Structures   Q97T38.dssp  





Publications:

Links:

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