Modomics - A Database of RNA Modifications

Full name:	Nucleolar protein 2
Synonym:	Yna1, N2428, YNL2428W
GI:	730166
Orf:	YNL061W
COG:	COG0144
UniProt:	P40991
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	l:2870(2501) - m5C

Comments:

The nucleolar Nop2 protein binds 27S pre-rRNA. Loss of m5C2870 affects ribosome synthesis and processing.

Protein sequence:

MGSRRHKNKQAAPPTLEEFQARKEKKANRKLEKGKRPSTTQGDEVSDRKKKKSKPFKKSRKEEEEVVEEDKDLPEVDLEELSKARKSLFDDEEDDDEAGL
VDEELKDEFDLEQEYDYDEDEDNDAHPIFSDDDDEADLEELNAQNMEALSKKLDEEEAEEAEEAEMELVEAENMQPRADILPTEEQEEMMAQETPNLTST
RTRMIEIVKVLENFKTLGAEGRSRGEYVDRLLKDICEYFGYTPFLAEKLFNLFSPAEAMEFFEANEIARPITIRTNTLKTRRRDLAQTLVNRGVNLQPIG
SWTKVGLQIFDSQVPIGATPEYLAGHYILQAASSFLPVIALDPHENERILDMAAAPGGKTTYISAMMKNTGCVFANDANKSRTKSLIANIHRLGCTNTIV
CNYDAREFPKVIGGFDRILLDAPCSGTGVIGKDQSVKVSRTEKDFIQIPHLQKQLLLSAIDSVDCNSKHGGVIVYSTCSVAVEEDEAVIDYALRKRPNVK
LVDTGLAIGKEAFTSYRGKKFHPSVKLARRYYPHTYNVDGFFVAKFQKIGPSSFDDNQASAKEKETAARKEALEEGIIHSDFATFEDEEDDKYIEKSVKN
NLLKKGVNPKAKRPSNEK

Enzymatic activities:

Reaction	Substrate	Type	Position
C:m5C	rRNA (r)	LSU-L/25S/eukaryotic cytosol	2870

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker.	de Beus E, Brockenbrough JS, Hong B, Aris JP	J Cell Biol	[details]	7806561	-
Nop2p is required for pre-rRNA processing and 60S ribosome subunit synthesis in yeast.	Hong B, Brockenbrough JS, Wu P, Aris JP	Mol Cell Biol	[details]	8972218	-
Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae.	Hong B, Wu K, Brockenbrough JS, Wu P, Aris JP	Nucleic Acids Res	[details]	11452018	-
Phylogenetic analysis of the eukaryotic RNA (cytosine-5)-methyltransferases.	Pavlopoulou A, Kossida S	Genomics	[details]	19135144	-
RNA methyltransferases utilize two cysteine residues in the formation of 5-methylcytosine.	King MY, Redman KL	Biochemistry	[details]	12220187	-
Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively.	Sharma S, Yang J, Watzinger P, Kotter P, Entian KD...	Nucleic Acids Res	[details]	23913415	-
The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits.	Sardana R, Johnson AW...	Mol Biol Cell	[details]	22956767	-