ID Card:
Full name:
tRNA-specific adenosine deaminase 3
Synonym:
ADAT3
GI:
74026040
Orf:
Tb11.01.6930
COG:
COG0590
UniProt:
Q381Q7
Structures:
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Alpha Fold Predicted Structure:
AF-Q381Q7-F1
Complex:
Tad2/3
Enzyme type:
deaminase
Position of modification - modification:
t :34 - I
Protein sequence:
MEEVVVPEEPPKLVSALATYVQQERLCTMFLSIANKLLPLKPHACHLKRIRRSSATRVATAPMDGFAGGVICDKRDSSVATSTISDGCERNSAALGTPAAENSHILELLLSVGGPVDSSALKELESAADTTVAVHRVWVPDRAPRSSAEEWTKWCQIWPFATPKPRAPTQLSECEMGSIQRIFRTVVMPLAKRLRTDETLGIAAVLVDPSDGYRVLVSSGEEHALKRGNSAACLGYVSNSGCRKSNRVVLDHPVTFVLKEVTRKQCKDREVEGDASYLANGMDMFVSHEPCVMCSMALVHSRVRRVFYCFPNPVHGGLGSTVSIHAIQELNHHFRVFRCDSRWLSDPEGVSSDHDNPYWEDLTVP
Comments:
Tad3 is part of the functional heterodimeric deaminase Tad2/3, where it contributes to catalysis. Tad3 is not simply a structural component of the dimeric enzyme. In T. brucei only one zinc ion out of two play a role in catalysis. Tad2/3 tRNA A:I editing enzyme plays a role in both A:I editing in position 34 and C:U editing in position 32 in vivo. In vitro, this A-to-I tRNA deaminase catalyzes also C-to-U deamination of DNA. The A34-to-I34 deamination reaction was tested with T. brucei tRNA-Thr as substrate. However, as other eukaryotic heterodimeric Tad2/3 it also works on other naturally occurring A34-containing tRNA (Alfonzo J. , unpublished data).
Alpha Fold Predicted Structure:
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Protein sequence:
M
E
E
V
V
V
P
E
E
P
P
K
L
V
S
A
L
A
T
Y
V
Q
Q
E
R
L
C
T
M
F
L
S
I
A
N
K
L
L
P
L
K
P
H
A
C
H
L
K
R
I
R
R
S
S
A
T
R
V
A
T
A
P
M
D
G
F
A
G
G
V
I
C
D
K
R
D
S
S
V
A
T
S
T
I
S
D
G
C
E
R
N
S
A
A
L
G
T
P
A
A
E
N
S
H
I
L
E
L
L
L
S
V
G
G
P
V
D
S
S
A
L
K
E
L
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S
A
A
D
T
T
V
A
V
H
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A
P
R
S
S
A
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T
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C
Q
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G
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G
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H
A
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G
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Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
A-to-I and C-to-U editing within transfer RNAs.
Su AA, Randau L
Biochemistry (Mosc)
[details]
22022967
-
Determinants of tRNA editing and modification: Avoiding conundrums, affecting function.
Paris Z, Fleming IM, Alfonzo JD
Semin Cell Dev Biol
[details]
22024020
-
A single zinc ion is sufficient for an active Trypanosoma brucei tRNA editing deaminase.
Spears JL, Rubio MA, Gaston KW, Wywial E, Strikoudis A, Bujnicki JM, Papavasiliou FN, Alfonzo JD
J Biol Chem
[details]
21507956
-
An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA.
Rubio MA, Pastar I, Gaston KW, Ragone FL, Janzen CJ, Cross GA, Papavasiliou FN, Alfonzo JD
Proc Natl Acad Sci U S A
[details]
17483465
-
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