Bud23 from Saccharomyces cerevisiae - protein summary.
Position of modification - modification:
s:1575(1338) - m7G
Bud23 methylates N7 atom of the highly conserved G1575 (m7G) located in a single stranded region between stem 29 and hairpin 42 of domain III in 18S rRNA (in E. coli it is the G1338, not methylated). Bud23 has a dual function, it is involved in both the processes of rRNA modification and nuclear export of small subunit pre-particles. Synthetic lethality was demonstrated with Rps15p, an essential ribosomal protein involved in nuclear export of small subunits pre-particles. Interaction with Trm112 is required for Bud23 activity.
Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits.
White J, Li Z, Sardana R, Bujnicki JM, Marcotte EM, Johnson AW
Mol Cell Biol
Genetic interactions show the importance of rRNA modification machinery for the role of Rps15p during ribosome biogenesis in S. cerevisiae.
Bellemer C, Chabosseau P, Gallardo F, Gleizes PE, Stahl G
Trm112 Is Required for Bud23-Mediated Methylation of the 18S rRNA at Position G1575.
Figaro S, Wacheul L, Schillewaert S, Graille M, Huvelle E, Mongeard R, Zorbas C, Lafontaine DL, Heurgue-Hamard V
Mol Cell Biol
Structural and functional studies of Bud23-Trm112 reveal 18S rRNA N7-G1575 methylation occurs on late 40S precursor ribosomes.
Letoquart J, Huvelle E, Wacheul L, Bourgeois G, Zorbas C, Graille M, Heurgue-Hamard V, Lafontaine DL...
Proc Natl Acad Sci U S A
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