ID Card:
Full name:
tRNA(Ile2) 2-agmatinylcytidine synthase TiaS
Synonym:
PF1855
GI:
18978227
COG:
COG1571
UniProt:
Q8TZX0
Structures:
|
3U02 |
Alpha Fold Predicted Structure:
AF-Q8TZX0-F1
Enzyme type:
agmatidine synthase
Position of modification - modification:
t :34 - C+
PDB Structures:
3U02
}
3U02
Structure Description:
Title:
Crystal Structure of the tRNA modifier TiaS from Pyrococcus furiosus, Northeast Structural Genomics Consortium Target PfR225
Classification:
TRANSCRIPTION
X-Ray Diffraction
2.4
0.242
0.182
0.179
Abstract of the PDB Structure's related Publication:
Download RCSB-PDB Structures:
Protein sequence:
MLIHIGIDDTDSPNGMCTTYIGAILYREISKIAEPLDFPRLIRLNPNVPYKTRGNGAVAMSFKIDEEKIKEVKTLVIRYVRELADIDHENTNPGIVFLIGEVPKELEEFSLRALREHVTIEEAEHVARKVNAEVYKFKLGRGIIGGLAAIGYPLEKFTYELLAYRKREYWGTPRRVIKESVFYADKWSYPFTYDNVDPYKRTVLITPHGKDPVLVGIRGIDVGKILQVFEMIKIEEPIEFFQVYKTNQNTDDHLVVKKIKDVKPYDNVVIRGKVAGKYWEKGRHVFFELEDETGKIRVAAFEPTKKFRNYVRKLLPGDEVIIAGGVKEFEGVLTVNLEKFYPVNLVPKVEYEKPRCPKCGGTMKSKGDYLKCKKCGYKMEKILTPVKRPRDLKKKIYEVPPDARKHISRPLVLPGGEDKILEAL
Comments:
Archaeal TiaS catalyzing formation of agmatidine plays the same decoding role as bacterial TilS catalyzing the formation of lysidine (k2C) in the wobble position of tRNAIle(CAU). Moreover in comparison to bacterial TilS, TiaS has an additional function (phosphorylation of C2 atom of the wobble cytidine-34 as well as autophosphorylation of Thr18 of TiaS). For predicted enzymatic activities see the entry for A. fulgidus TiaS.
Alpha Fold Predicted Structure:
Parsing response... [403296/403296]
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Protein sequence:
M
L
I
H
I
G
I
D
D
T
D
S
P
N
G
M
C
T
T
Y
I
G
A
I
L
Y
R
E
I
S
K
I
A
E
P
L
D
F
P
R
L
I
R
L
N
P
N
V
P
Y
K
T
R
G
N
G
A
V
A
M
S
F
K
I
D
E
E
K
I
K
E
V
K
T
L
V
I
R
Y
V
R
E
L
A
D
I
D
H
E
N
T
N
P
G
I
V
F
L
I
G
E
V
P
K
E
L
E
E
F
S
L
R
A
L
R
E
H
V
T
I
E
E
A
E
H
V
A
R
K
V
N
A
E
V
Y
K
F
K
L
G
R
G
I
I
G
G
L
A
A
I
G
Y
P
L
E
K
F
T
Y
E
L
L
A
Y
R
K
R
E
Y
W
G
T
P
R
R
V
I
K
E
S
V
F
Y
A
D
K
W
S
Y
P
F
T
Y
D
N
V
D
P
Y
K
R
T
V
L
I
T
P
H
G
K
D
P
V
L
V
G
I
R
G
I
D
V
G
K
I
L
Q
V
F
E
M
I
K
I
E
E
P
I
E
F
F
Q
V
Y
K
T
N
Q
N
T
D
D
H
L
V
V
K
K
I
K
D
V
K
P
Y
D
N
V
V
I
R
G
K
V
A
G
K
Y
W
E
K
G
R
H
V
F
F
E
L
E
D
E
T
G
K
I
R
V
A
A
F
E
P
T
K
K
F
R
N
Y
V
R
K
L
L
P
G
D
E
V
I
I
A
G
G
V
K
E
F
E
G
V
L
T
V
N
L
E
K
F
Y
P
V
N
L
V
P
K
V
E
Y
E
K
P
R
C
P
K
C
G
G
T
M
K
S
K
G
D
Y
L
K
C
K
K
C
G
Y
K
M
E
K
I
L
T
P
V
K
R
P
R
D
L
K
K
K
I
Y
E
V
P
P
D
A
R
K
H
I
S
R
P
L
V
L
P
G
G
E
D
K
I
L
E
A
L
50 100 150 200 250 300 350 400 Sequence G H T B S N
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications: