Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA(Ile2) 2-agmatinylcytidine synthase TiaS
Synonym: PF1855
GI: 18978227
COG: COG1571
UniProt: Q8TZX0
Structures: | 3U02 |
Alpha Fold Predicted Structure: AF-Q8TZX0-F1
Enzyme type: agmatidine synthase
Position of modification - modification: t:34 - C+


PDB Structures:


3U02

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   3U02.pdb  
Pdbx/mmCIF Files   3U02.cif  


Protein sequence:

MLIHIGIDDTDSPNGMCTTYIGAILYREISKIAEPLDFPRLIRLNPNVPYKTRGNGAVAMSFKIDEEKIKEVKTLVIRYVRELADIDHENTNPGIVFLIGEVPKELEEFSLRALREHVTIEEAEHVARKVNAEVYKFKLGRGIIGGLAAIGYPLEKFTYELLAYRKREYWGTPRRVIKESVFYADKWSYPFTYDNVDPYKRTVLITPHGKDPVLVGIRGIDVGKILQVFEMIKIEEPIEFFQVYKTNQNTDDHLVVKKIKDVKPYDNVVIRGKVAGKYWEKGRHVFFELEDETGKIRVAAFEPTKKFRNYVRKLLPGDEVIIAGGVKEFEGVLTVNLEKFYPVNLVPKVEYEKPRCPKCGGTMKSKGDYLKCKKCGYKMEKILTPVKRPRDLKKKIYEVPPDARKHISRPLVLPGGEDKILEAL

Comments:

Archaeal TiaS catalyzing formation of agmatidine plays the same decoding role as bacterial TilS catalyzing the formation of lysidine (k2C) in the wobble position of tRNAIle(CAU). Moreover in comparison to bacterial TilS, TiaS has an additional function (phosphorylation of C2 atom of the wobble cytidine-34 as well as autophosphorylation of Thr18 of TiaS). For predicted enzymatic activities see the entry for A. fulgidus TiaS.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M L I H I G I D D T D S P N G M C T T Y I G A I L Y R E I S K I A E P L D F P R L I R L N P N V P Y K T R G N G A V A M S F K I D E E K I K E V K T L V I R Y V R E L A D I D H E N T N P G I V F L I G E V P K E L E E F S L R A L R E H V T I E E A E H V A R K V N A E V Y K F K L G R G I I G G L A A I G Y P L E K F T Y E L L A Y R K R E Y W G T P R R V I K E S V F Y A D K W S Y P F T Y D N V D P Y K R T V L I T P H G K D P V L V G I R G I D V G K I L Q V F E M I K I E E P I E F F Q V Y K T N Q N T D D H L V V K K I K D V K P Y D N V V I R G K V A G K Y W E K G R H V F F E L E D E T G K I R V A A F E P T K K F R N Y V R K L L P G D E V I I A G G V K E F E G V L T V N L E K F Y P V N L V P K V E Y E K P R C P K C G G T M K S K G D Y L K C K K C G Y K M E K I L T P V K R P R D L K K K I Y E V P P D A R K H I S R P L V L P G G E D K I L E A L

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q8TZX0-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q8TZX0-F1.cif  
DSSP Secondary Structures   Q8TZX0.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea. Ikeuchi Y, Kimura S, Numata T, Nakamura D, Yokogawa T, Ogata T, Wada T, Suzuki T, Suzuki T Nat Chem Biol [details] 20139989 -
Structural basis of tRNA agmatinylation essential for AUA codon decoding. Osawa T, Kimura S, Terasaka N, Inanaga H, Suzuki T, Numata T Nat Struct Mol Biol [details] 22002223 -
Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS. Terasaka N, Kimura S, Osawa T, Numata T, Suzuki T Nat Struct Mol Biol [details] 22002222 -