Modomics - A Database of RNA Modifications

ID Card:

Full name:	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
Synonym:	YmcB, BSU17010
GI:	16078764
COG:	COG0621
UniProt:	O31778
Alpha Fold Predicted Structure:	AF-O31778-F1
Enzyme type:	methylthiotransferase predicted

Protein sequence:

MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFKGLGDGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLSEAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRRPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTTSHPRDFDDRLIEVLAKGGNLLDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVKKERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEAIGKIVRVKIQQAKTWSLDGEMVGEAIEVK

Comments:

Corresponds to E. coli MiaB ortholog. Posesses a N-terminal TRAM domain and a C-terminal catalytic radical SAM domain with two [4Fe-4S] clusters coordinated with 3 cysteines and an exchangeable S-AdoMet. Belongs to the same family of methylthiotransferases as B. subtilis MtaB (YqeV), mammalian MtaB (Cdkal1) and protein methylthiotransferase RimO. A MiaB-CDKAL1-like enzyme is present in Archaea, while MiaA/Mod5 is totally absent. Its function is still elusive (2012).

Reaction	Substrate	SubstrateType	Position	(Anti)Codon	Modified (Anti)Codon	Amino Acid Change	Transcript Name	Transcript Region	Cellular Localization	References
i6A:ms2i6A	tRNA (t)	many/many/prokaryotic cytosol	37							20472640

Alpha Fold Predicted Structure:

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Protein sequence:

Secondary Structure Alphabet

G: 3-turn helix (3₁₀helix)
H: α-helix
I: 𝝅-helix (5 - turn helix)
T: Hydrogen Bonded Turn
B: β-sheet
S: Bend
C: Coil (residues not present in any of the above conformations)
N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files	AF-O31778-F1.pdb
Alpha Fold Pdbx/mmCIF Files	AF-O31778-F1.cif
DSSP Secondary Structures	O31778.dssp

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Structural bioinformatics analysis of enzymes involved in the biosynthesis pathway of the hypermodified nucleoside ms(2)io(6)A37 in tRNA.	Kaminska KH, Baraniak U, Boniecki M, Nowaczyk K, Czerwoniec A, Bujnicki JM	Proteins	[details]	17910062	-
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.	Anton BP, Russell SP, Vertrees J, Kasif S, Raleigh EA, Limbach PA, Roberts RJ	Nucleic Acids Res	[details]	20472640	-
The methylthiolation reaction mediated by the Radical-SAM enzymes.	Atta M, Arragain S, Fontecave M, Mulliez E, Hunt JF, Luff JD, Forouhar F	Biochim Biophys Acta	[details]	22178611	-

Links:

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