Modomics - A Database of RNA Modifications

ID Card:

Full name: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
Synonym: YmcB, BSU17010
GI: 16078764
COG: COG0621
UniProt: O31778
Alpha Fold Predicted Structure: AF-O31778-F1
Enzyme type: methylthiotransferase predicted



Protein sequence:

MNEKQKLESGQVHPSDKKSEKDYSKYFEAVYIPPSLKDAKKRGKEAVTYHNDFKISEQFKGLGDGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLSEAYLSKEMVVEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRRPEDIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTTSHPRDFDDRLIEVLAKGGNLLDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVKKERLQRLNALVNEISAKKMKEYEGKVVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKEAIGKIVRVKIQQAKTWSLDGEMVGEAIEVK

Comments:

Corresponds to E. coli MiaB ortholog. Posesses a N-terminal TRAM domain and a C-terminal catalytic radical SAM domain with two [4Fe-4S] clusters coordinated with 3 cysteines and an exchangeable S-AdoMet. Belongs to the same family of methylthiotransferases as B. subtilis MtaB (YqeV), mammalian MtaB (Cdkal1) and protein methylthiotransferase RimO. A MiaB-CDKAL1-like enzyme is present in Archaea, while MiaA/Mod5 is totally absent. Its function is still elusive (2012).





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:


50100150200250300350400450500SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-O31778-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-O31778-F1.cif  
DSSP Secondary Structures   O31778.dssp  





Publications:

Links:

_PubMed_