ID Card:
Full name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
Synonym:
TM0653
GI:
15643418
COG:
COG0621
UniProt:
Q9WZC1
Alpha Fold Predicted Structure:
AF-Q9WZC1-F1
Enzyme type:
methylthiotransferase predicted
Position of modification - modification:
t :37 - ms2i6A
Protein sequence:
MRFYIKTFGCQMNENDSEAMAGLLVKEGFTPASSPEEADVVIINTCAVRRKSEEKAYSELGQVLKLKKKKKIVVGVAGCVAEKEREKFLEKGADFVLGTRAVPRVTEAVKKALEGEKVALFEDHLDEYTHELPRIRTSRHHAWVTIIHGCDRFCTYCIVPYTRGRERSRPMADILEEVKKLAEQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFLTSYPTDFSDELIEVIAKNPKVAKSVHLPVQSGSNRILKLMNRRYTKEEYLALLEKIRSKVPEVAISSDIIVGFPTETEEDFMETVDLVEKAQFERLNLAIYSPREGTVAWKYYKDDVPYEEKVRRMQFLMNLQKRINRKLNERYRGKTVRIIVEAQAKNGLFYGRDIRNKIIAFEGEDWMIGRFADVKVEKITAGPLYGKVVWVEKTPSPVSSSE
Comments:
Bacterial MiaB works on A36-i6A37-containing tRNA. It displays a N-terminal TRAM domain and a C-terminal catalytic radical SAM domain with two [4Fe-4S] clusters coordinated with 3 cysteines and an exchangeable S-AdoMet. Belongs to the same family of methylthiotransferases as B subtilis MtaB (YqeV), mammalian MtaB (Cdkal1) and protein methylthiotransferase RimO. A MiaB-CDKAL1-like enzyme is present in Archaea, while MiaA/Mod5 is totally absent. Its function is still elusive (2012).
Alpha Fold Predicted Structure:
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Protein sequence:
M
R
F
Y
I
K
T
F
G
C
Q
M
N
E
N
D
S
E
A
M
A
G
L
L
V
K
E
G
F
T
P
A
S
S
P
E
E
A
D
V
V
I
I
N
T
C
A
V
R
R
K
S
E
E
K
A
Y
S
E
L
G
Q
V
L
K
L
K
K
K
K
K
I
V
V
G
V
A
G
C
V
A
E
K
E
R
E
K
F
L
E
K
G
A
D
F
V
L
G
T
R
A
V
P
R
V
T
E
A
V
K
K
A
L
E
G
E
K
V
A
L
F
E
D
H
L
D
E
Y
T
H
E
L
P
R
I
R
T
S
R
H
H
A
W
V
T
I
I
H
G
C
D
R
F
C
T
Y
C
I
V
P
Y
T
R
G
R
E
R
S
R
P
M
A
D
I
L
E
E
V
K
K
L
A
E
Q
G
Y
R
E
V
T
F
L
G
Q
N
V
D
A
Y
G
K
D
L
K
D
G
S
S
L
A
K
L
L
E
E
A
S
K
I
E
G
I
E
R
I
W
F
L
T
S
Y
P
T
D
F
S
D
E
L
I
E
V
I
A
K
N
P
K
V
A
K
S
V
H
L
P
V
Q
S
G
S
N
R
I
L
K
L
M
N
R
R
Y
T
K
E
E
Y
L
A
L
L
E
K
I
R
S
K
V
P
E
V
A
I
S
S
D
I
I
V
G
F
P
T
E
T
E
E
D
F
M
E
T
V
D
L
V
E
K
A
Q
F
E
R
L
N
L
A
I
Y
S
P
R
E
G
T
V
A
W
K
Y
Y
K
D
D
V
P
Y
E
E
K
V
R
R
M
Q
F
L
M
N
L
Q
K
R
I
N
R
K
L
N
E
R
Y
R
G
K
T
V
R
I
I
V
E
A
Q
A
K
N
G
L
F
Y
G
R
D
I
R
N
K
I
I
A
F
E
G
E
D
W
M
I
G
R
F
A
D
V
K
V
E
K
I
T
A
G
P
L
Y
G
K
V
V
W
V
E
K
T
P
S
P
V
S
S
S
E
50 100 150 200 250 300 350 400 Sequence G H T B S N
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
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