Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA dimethylallyltransferase, mitochondrial
Synonym: TRIT1, IPT
GI: 31581534
COG: COG0324
UniProt: Q9H3H1
Alpha Fold Predicted Structure: AF-Q9H3H1-F1
Enzyme type: dimethylallyltransferase
Position of modification - modification: t:37 - i6A



Protein sequence:

MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVYEGLDIITNKVSAQEQRICRHHMISFVDPLVTNYTVVDFRNRATALIEDIFARDKIPIVVGGTNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Comments:

Mod5 catalyzes the transfer the delta2-isopropyl group from dimethylallyl diphosphate to the exocyclic N6 of A36A37-containing tRNA (i6A37 formation). Eukaryotic Mod5 is the ortholog of bacterial MiaA. There are no MiaA/Mod5 homologs in Archaea. Homo sapiens derived gene was shown to complement S. cerevisiae mutant lacking Mod5.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
A:i6A tRNA (t) many/many/mitochondrion 37 11111046   
A:i6A RNA tRNA 36 AGA AGA tRNASerAGA anticodon Cytoplasm 24126054   
A:i6A RNA tRNA 36 CGA CGA tRNASerCGA anticodon Cytoplasm 24126054   
A:i6A RNA tRNA UGA UGA tRNASerUGA anticodon Cytoplasm 24126054   
A:i6A RNA tRNA 36 tRNASecUCA anticodon Cytoplasm 24126054   
A:i6A RNA tRNA 36 UGA UGA tRNASerUGA anticodon Mitochondrion 24126054   
A:i6A RNA tRNA 36 UCA UCA tRNATrpUCA anticodn Mitochondrion 24126054   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A S V A A A R A V P V G S G L R G L Q R T L P L V V I L G A T G T G K S T L A L Q L G Q R L G G E I V S A D S M Q V Y E G L D I I T N K V S A Q E Q R I C R H H M I S F V D P L V T N Y T V V D F R N R A T A L I E D I F A R D K I P I V V G G T N Y Y I E S L L W K V L V N T K P Q E M G T E K V I D R K V E L E K E D G L V L H K R L S Q V D P E M A A K L H P H D K R K V A R S L Q V F E E T G I S H S E F L H R Q H T E E G G G P L G G P L K F S N P C I L W L H A D Q A V L D E R L D K R V D D M L A A G L L E E L R D F H R R Y N Q K N V S E N S Q D Y Q H G I F Q S I G F K E F H E Y L I T E G K C T L E T S N Q L L K K G I E A L K Q V T K R Y A R K Q N R W V K N R F L S R P G P I V P P V Y G L E V S D V S K W E E S V L E P A L E I V Q S F I Q G H K P T A T P I K M P Y N E A E N K R S Y H L C D L C D R I I I G D R E W A A H I K S K S H L N Q L K K R R R L D S D A V N T I E S Q S V S P D H N K E P K E K G S P G Q N D Q E L K C S V

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9H3H1-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9H3H1-F1.cif  
DSSP Secondary Structures   Q9H3H1.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Cloning of a human tRNA isopentenyl transferase. Golovko A, Hjalm G, Sitbon F, Nicander B Gene [details] 11111046 -

Links:

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