Modomics - A Database of RNA Modifications

ID Card:

Full name: Threonylcarbamoyladenosine tRNA methylthiotransferase
Synonym: tRNA-t(6)A37 methylthiotransferase, Cdkal1
GI: 21617853
COG: COG0621
UniProt: Q91WE6
Alpha Fold Predicted Structure: AF-Q91WE6-F1
Enzyme type: methylthiotransferase
Position of modification - modification: t:37 - ms2t6A



Protein sequence:

MPSASCDVLLDDIEDIISQEDSKPQDRQFSRKHVFPKVRRRNTQKYLQEEPRPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAEQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSISKPLAKGEVSGLTKEFRNRLGNHPNGTSDTCPATQHGSAYSRMVLQMSQYDCALKVATGLALLALLLHFWPDSLLTM

Comments:

ms2t6A is found at position 37 of tRNA-Lys(UUU) only. Eukaryotic MtaB (Cdkal1) displays C-terminal TRAM domain and 2 [4Fe-4S] clusters), characteristic features of the methylthiotransferase family, a subclass of the large radical AdoMet enzyme superfamily, including B. subtilis MtaB (YqeV) and E. coli or B. subtilis MiaE.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
t6A:ms2t6A tRNA (t) Lys/SUU/prokaryotic cytosol 37 21841312   
t6A:ms2t6A RNA tRNA 37 AGT AGT tRNAThrAGT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 CGT CGT tRNAThrCGT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 TGT TGT tRNAThrTGT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 GTT GTT tRNAAsnGTT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 CTT CTT tRNALysCTT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 TTT TTT tRNALysTTT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 GCT GCT tRNASerGCT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 CCT CCT tRNAArgCCT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 TCT TCT tRNAArgTCT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 AAT AAT tRNAIleAAT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 TAT TAT tRNAIleTAT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   
t6A:ms2t6A RNA tRNA 37 CAT CAT tRNAiMet/MetCAT anticodon-loop Endoplasmatic Reticulum Membrane 20584901   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M P S A S C D V L L D D I E D I I S Q E D S K P Q D R Q F S R K H V F P K V R R R N T Q K Y L Q E E P R P P S D S T I P G I Q K I W I R T W G C S H N N S D G E Y M A G Q L A A Y G Y K I T E N A S D A D L W L L N S C T V K N P A E D H F R N S I K K A Q E E N K K V V L A G C V P Q A Q P R Q D Y L K G L S I I G V Q Q I D R V V E V V E E T I K G H S V R L L G Q K K D N G K R L G G A R L D L P K I R K N P L I E I I S I N T G C L N A C T Y C K T K H A R G N L A S Y P I D E L V E R A K Q S F Q E G V C E I W L T S E D T G A Y G R D I G T D L P T L L W K L V E V I P E G A M L R L G M T N P P Y I L E H L E E M A K I L N H P R V Y A F L H I P V Q S A S D S V L M D M K R E Y C V A D F K R V V D F L K E K V P G I T I A T D I I C G F P G E T D Q D F Q E T V K L V E E Y K F P S L F I N Q F Y P R P G T P A A K A E Q V P A H V K K Q R T K D L S R V F H S Y N P Y D H K I G E R Q Q V L V T E E S F D S K F Y V A H N R F Y E Q V L V P K N P A F M G K M V E V D I Y E S G K H F L K G Q P V S E T R V Y T P S I S K P L A K G E V S G L T K E F R N R L G N H P N G T S D T C P A T Q H G S A Y S R M V L Q M S Q Y D C A L K V A T G L A L L A L L L H F W P D S L L T M

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q91WE6-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q91WE6-F1.cif  
DSSP Secondary Structures   Q91WE6.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA. Arragain S, Handelman SK, Forouhar F, Wei FY, Tomizawa K, Hunt JF, Douki T, Fontecave M, Mulliez E, Atta M J Biol Chem [details] 20584901 -
Deficit of tRNA(Lys) modification by Cdkal1 causes the development of type 2 diabetes in mice. Wei FY, Suzuki T, Watanabe S, Kimura S, Kaitsuka T, Fujimura A, Matsui H, Atta M, Michiue H, Fontecave M, Yamagata K, Suzuki T, Tomizawa K J Clin Invest [details] 21841312 -

Links:

_PubMed_