Modomics - A Database of RNA Modifications

ID Card:

Full name: 23S rRNA (guanine(748)-N(1))-methyltransferase
Synonym: TlrB
GI: 4210927
COG: COG2226
UniProt: Q9S1M6
Alpha Fold Predicted Structure: AF-Q9S1M6-F1
Enzyme type: methyltransferase
Position of modification - modification: l:748(748) - m1G



Protein sequence:

MRKNVVRYLRCPHCAAPLRSSDRTLRCENGHTFDVARQGYVNLLRRPTKLAADTTDMVAARAALLDSGHYAPLTERLAGTAGRAAGAGAPDCVVDIGGGTGHHLARVLEEFEDAEGLLLDMSKPAVRRAARAHPRASSAVADVWDTLPLRDGAAAMALNVFAPRNPPEIRRILRPGGTLLVVTPQQDHLAELVDALGLLRVRDHKEGRLAEQLAPHFEAVGQERLRTTLRLDHDALGRVVAMGPSSWHQDPDELARRIAELPGIHEVTLSVTFTVCRPLP

Comments:

RlmA(II) is found only in several groups of gram positive bacteria (Steptomyces and Streptococcus). It catalyzes the formation of m1G at position 748 in the loop of helix 35 of large subunit rRNA. It confers resistance to tylosin and other mycinosylated macrolide antibiotics. In vitro it works on naked 23S rRNA. AdoMet is the donor of methyl group. RlmA(II) is the ortholog of RlmA(I) that is found only in gram negative bacteria (such as E. coli). Methylation site was determined for E. coli rRNA.





Alpha Fold Predicted Structure:




Parsing response... [254342/254342]


Clear Selection and Reset Camera

Protein sequence:

M R K N V V R Y L R C P H C A A P L R S S D R T L R C E N G H T F D V A R Q G Y V N L L R R P T K L A A D T T D M V A A R A A L L D S G H Y A P L T E R L A G T A G R A A G A G A P D C V V D I G G G T G H H L A R V L E E F E D A E G L L L D M S K P A V R R A A R A H P R A S S A V A D V W D T L P L R D G A A A M A L N V F A P R N P P E I R R I L R P G G T L L V V T P Q Q D H L A E L V D A L G L L R V R D H K E G R L A E Q L A P H F E A V G Q E R L R T T L R L D H D A L G R V V A M G P S S W H Q D P D E L A R R I A E L P G I H E V T L S V T F T V C R P L P
20406080100120140160180200220240260280SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9S1M6-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9S1M6-F1.cif  
DSSP Secondary Structures   Q9S1M6.dssp  





Publications:

Links:

_PubMed_