ID Card:
| Full name: |
Dimethyladenosine transferase 1, mitochondrial |
| Synonym: |
DIMT1, h-mtTFB1 |
| GI: |
74751555
|
| Orf: |
CGI-75 |
| COG: |
COG0030 |
| UniProt: |
Q8WVM0 |
| Structures: |
|
|
|
| Enzyme type: |
methyltransferase |
Protein sequence:
MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL
Comments:
h-mtTFB1 was first decribed as a nuclear-encoded mitochondrial transcription factor, but this protein has a dual function: acts as a mitochondrial transcription factor and an rRNA-modification enzyme. It methylates a conserved tandem A's (positions 1518, 1519, E. coli numbering) in the loop of a hairpin near the 3'-end of bacterial small subunit rRNA (as bacterial RsmA, formely KsgA). It was shown that the homologous sequence in the human mitochondrial 12S molecule is similarly modified. A second h-mtTFB2 also exists that is less efficient at methylating rRNA than h-mtTFB1. AdoMet is probably the methyl group donor. The structure of probable human cytosolic dimethyladenosine transferase has been solved (PDB id: 1ZQ9)
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop. |
Seidel-Rogol BL, McCulloch V, Shadel GS |
Nat Genet |
[details]
|
12496758
|
-
|
| Comprehensive phylogenetic analysis of evolutionarily conserved rRNA adenine dimethyltransferase suggests diverse bacterial contributions to the nucleus-encoded plastid proteome. |
Park AK, Kim H, Jin HJ |
Mol Phylogenet Evol |
[details]
|
19017544
|
-
|
| Evidence for an early gene duplication event in the evolution of the mitochondrial transcription factor B family and maintenance of rRNA methyltransferase activity in human mtTFB1 and mtTFB2. |
Cotney J, Shadel GS |
J Mol Evol |
[details]
|
17031457
|
-
|
Links: