ID Card:
Full name:
RNA small subunit Methyltransferase B
Synonym:
TTHA0851
GI:
55980820
COG:
COG0144
UniProt:
Q5SK01
Alpha Fold Predicted Structure:
AF-Q5SK01-F1
Enzyme type:
methyltransferase predicted
Protein sequence:
MRAGTPRALAVAVLLEVDRGGRAQLLLDRALRRSPWPERDRAYATFLVYGALRRLRLLDHLLAPLLPRPEGLPPEVRWILRLGALEWLEGKPDHARVSPWVEEAKRRYPGLAGLVNAVLRRLAPREAPECVRLSLPDWLCEAWRGFFGDVAFAEGFNEPAPLFVTAYREVDLRPGPVPGSYLWEGPKTDFSALGLQPQNPASLFAAKLLEARPGERVLDLCGGAGLKAFYLAAQGAEVVSYDLNRRRQEAGARTARRLGLWVHYRTQDLTRPVPERAKKVLLDAPCTGTGTFRAHPELRYRLSPEDPARMAALQLQLLETAAQATEEGGVLVYSVCTLTEEEGEGVVRAFLARHPEFRPEPVQPPFPVLARGLGVYVDPRGGLDGFYYAKLRRVNSQA
Comments:
Like its E. coli ortholog, T. thermophilus RsmB catalyzes the site-specific formation of m5C at position 967 (E. coli numbering) in the loop of helix 31 in 16S rRNA (adjacent to m2G966 catalyzed by RsmD). AdoMet is the methyl group donor. Together with the multi-site specific T. thermophilus RsmF, all four 5-methylcytidine (m5C) modifications are synthesized in T. thermophilus 16S rRNA. RsmB belongs to the same methyltransferase family as E.coli RsmF, yeast Nop2, yeast and archaeal Trm4, and homo sapiens P120, NSUN1, NSUN2, NSUN3 and NSUN4.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
C:m5C
rRNA (r)
SSU/16S/prokaryotic cytosol
945
20558545
Alpha Fold Predicted Structure:
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Protein sequence:
M
R
A
G
T
P
R
A
L
A
V
A
V
L
L
E
V
D
R
G
G
R
A
Q
L
L
L
D
R
A
L
R
R
S
P
W
P
E
R
D
R
A
Y
A
T
F
L
V
Y
G
A
L
R
R
L
R
L
L
D
H
L
L
A
P
L
L
P
R
P
E
G
L
P
P
E
V
R
W
I
L
R
L
G
A
L
E
W
L
E
G
K
P
D
H
A
R
V
S
P
W
V
E
E
A
K
R
R
Y
P
G
L
A
G
L
V
N
A
V
L
R
R
L
A
P
R
E
A
P
E
C
V
R
L
S
L
P
D
W
L
C
E
A
W
R
G
F
F
G
D
V
A
F
A
E
G
F
N
E
P
A
P
L
F
V
T
A
Y
R
E
V
D
L
R
P
G
P
V
P
G
S
Y
L
W
E
G
P
K
T
D
F
S
A
L
G
L
Q
P
Q
N
P
A
S
L
F
A
A
K
L
L
E
A
R
P
G
E
R
V
L
D
L
C
G
G
A
G
L
K
A
F
Y
L
A
A
Q
G
A
E
V
V
S
Y
D
L
N
R
R
R
Q
E
A
G
A
R
T
A
R
R
L
G
L
W
V
H
Y
R
T
Q
D
L
T
R
P
V
P
E
R
A
K
K
V
L
L
D
A
P
C
T
G
T
G
T
F
R
A
H
P
E
L
R
Y
R
L
S
P
E
D
P
A
R
M
A
A
L
Q
L
Q
L
L
E
T
A
A
Q
A
T
E
E
G
G
V
L
V
Y
S
V
C
T
L
T
E
E
E
G
E
G
V
V
R
A
F
L
A
R
H
P
E
F
R
P
E
P
V
Q
P
P
F
P
V
L
A
R
G
L
G
V
Y
V
D
P
R
G
G
L
D
G
F
Y
Y
A
K
L
R
R
V
N
S
Q
A
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus.
Demirci H, Larsen LH, Hansen T, Rasmussen A, Cadambi A, Gregory ST, Kirpekar F, Jogl G
RNA
[details]
20558545
-
Links: